Phospho-MLK1/2 (Thr312/Thr266) Antibody - #AF3630
| 製品: | Phospho-MLK1/2 (Thr312/Thr266) Antibody |
| カタログ: | AF3630 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-MLK1/2 (Thr312/Thr266) |
| アプリケーション: | ELISA(peptide) |
| 反応性: | Human, Mouse, Rat |
| 分子量: | 122kD,104kD(Calculated). |
| ユニプロット: | P80192 | Q02779 |
| RRID: | AB_2846944 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF3630, RRID:AB_2846944.
折りたたみ／展開
M3K9_HUMAN; Map3k9; MEKK9; Mitogen activated protein kinase kinase kinase 9; Mitogen-activated protein kinase kinase kinase 9; Mixed lineage kinase 1 (tyr and ser/thr specificity); Mixed lineage kinase 1; MLK1; PRKE1; M3K10_HUMAN; MAP3K 10; Map3k10; MEKK10; Mitogen activated protein kinase kinase kinase 10; Mitogen-activated protein kinase kinase kinase 10; Mixed lineage kinase 2; MKN28 derived nonreceptor type serine/threonine kinase; MKN28 kinase; MLK 2; MST; Protein kinase MST;
免疫原
A synthesized peptide derived from human MLK1/2 around the phosphorylation site of Thr312/266.
Expressed in epithelial tumor cell lines of colonic, breast and esophageal origin.
Q02779 M3K10_HUMAN:Expressed in brain and skeletal muscle.
- P80192 M3K9_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEPSRALLGCLASAAAAAPPGEDGAGAGAEEEEEEEEEAAAAVGPGELGCDAPLPYWTAVFEYEAAGEDELTLRLGDVVEVLSKDSQVSGDEGWWTGQLNQRVGIFPSNYVTPRSAFSSRCQPGGEDPSCYPPIQLLEIDFAELTLEEIIGIGGFGKVYRAFWIGDEVAVKAARHDPDEDISQTIENVRQEAKLFAMLKHPNIIALRGVCLKEPNLCLVMEFARGGPLNRVLSGKRIPPDILVNWAVQIARGMNYLHDEAIVPIIHRDLKSSNILILQKVENGDLSNKILKITDFGLAREWHRTTKMSAAGTYAWMAPEVIRASMFSKGSDVWSYGVLLWELLTGEVPFRGIDGLAVAYGVAMNKLALPIPSTCPEPFAKLMEDCWNPDPHSRPSFTNILDQLTTIEESGFFEMPKDSFHCLQDNWKHEIQEMFDQLRAKEKELRTWEEELTRAALQQKNQEELLRRREQELAEREIDILERELNIIIHQLCQEKPRVKKRKGKFRKSRLKLKDGNRISLPSDFQHKFTVQASPTMDKRKSLINSRSSPPASPTIIPRLRAIQLTPGESSKTWGRSSVVPKEEGEEEEKRAPKKKGRTWGPGTLGQKELASGDEGSPQRREKANGLSTPSESPHFHLGLKSLVDGYKQWSSSAPNLVKGPRSSPALPGFTSLMEMEDEDSEGPGSGESRLQHSPSQSYLCIPFPRGEDGDGPSSDGIHEEPTPVNSATSTPQLTPTNSLKRGGAHHRRCEVALLGCGAVLAATGLGFDLLEAGKCQLLPLEEPEPPAREEKKRREGLFQRSSRPRRSTSPPSRKLFKKEEPMLLLGDPSASLTLLSLSSISECNSTRSLLRSDSDEIVVYEMPVSPVEAPPLSPCTHNPLVNVRVERFKRDPNQSLTPTHVTLTTPSQPSSHRRTPSDGALKPETLLASRSPSSNGLSPSPGAGMLKTPSPSRDPGEFPRLPDPNVVFPPTPRRWNTQQDSTLERPKTLEFLPRPRPSANRQRLDPWWFVSPSHARSTSPANSSSTETPSNLDSCFASSSSTVEERPGLPALLPFQAGPLPPTERTLLDLDAEGQSQDSTVPLCRAELNTHRPAPYEIQQEFWS
- Q02779 M3K10_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEEEEGAVAKEWGTTPAGPVWTAVFDYEAAGDEELTLRRGDRVQVLSQDCAVSGDEGWWTGQLPSGRVGVFPSNYVAPGAPAAPAGLQLPQEIPFHELQLEEIIGVGGFGKVYRALWRGEEVAVKAARLDPEKDPAVTAEQVCQEARLFGALQHPNIIALRGACLNPPHLCLVMEYARGGALSRVLAGRRVPPHVLVNWAVQVARGMNYLHNDAPVPIIHRDLKSINILILEAIENHNLADTVLKITDFGLAREWHKTTKMSAAGTYAWMAPEVIRLSLFSKSSDVWSFGVLLWELLTGEVPYREIDALAVAYGVAMNKLTLPIPSTCPEPFARLLEECWDPDPHGRPDFGSILKRLEVIEQSALFQMPLESFHSLQEDWKLEIQHMFDDLRTKEKELRSREEELLRAAQEQRFQEEQLRRREQELAEREMDIVERELHLLMCQLSQEKPRVRKRKGNFKRSRLLKLREGGSHISLPSGFEHKITVQASPTLDKRKGSDGASPPASPSIIPRLRAIRLTPVDCGGSSSGSSSGGSGTWSRGGPPKKEELVGGKKKGRTWGPSSTLQKERVGGEERLKGLGEGSKQWSSSAPNLGKSPKHTPIAPGFASLNEMEEFAEAEDGGSSVPPSPYSTPSYLSVPLPAEPSPGARAPWEPTPSAPPARWGHGARRRCDLALLGCATLLGAVGLGADVAEARAADGEEQRRWLDGLFFPRAGRFPRGLSPPARPHGRREDVGPGLGLAPSATLVSLSSVSDCNSTRSLLRSDSDEAAPAAPSPPPSPPAPTPTPSPSTNPLVDLELESFKKDPRQSLTPTHVTAACAVSRGHRRTPSDGALGQRGPPEPAGHGPGPRDLLDFPRLPDPQALFPARRRPPEFPGRPTTLTFAPRPRPAASRPRLDPWKLVSFGRTLTISPPSRPDTPESPGPPSVQPTLLDMDMEGQNQDSTVPLCGAHGSH
PTMs - P80192/Q02779 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y110 | Phosphorylation | Uniprot | |
| K193 | Ubiquitination | Uniprot | |
| T304 | Phosphorylation | P80192 (MAP3K9) | Uniprot |
| T305 | Phosphorylation | P80192 (MAP3K9) | Uniprot |
| S308 | Phosphorylation | P80192 (MAP3K9) | Uniprot |
| T312 | Phosphorylation | P80192 (MAP3K9) | Uniprot |
| T452 | Phosphorylation | Uniprot | |
| S519 | Phosphorylation | Uniprot | |
| S533 | Phosphorylation | Uniprot | |
| T535 | Phosphorylation | Uniprot | |
| S547 | Phosphorylation | Uniprot | |
| S548 | Phosphorylation | Uniprot | |
| S552 | Phosphorylation | Uniprot | |
| T554 | Phosphorylation | Uniprot | |
| T565 | Phosphorylation | Uniprot | |
| K594 | Acetylation | Uniprot | |
| K595 | Acetylation | Uniprot | |
| K607 | Acetylation | Uniprot | |
| S616 | Phosphorylation | Uniprot | |
| S652 | Phosphorylation | Uniprot | |
| K658 | Ubiquitination | Uniprot | |
| S693 | Phosphorylation | Uniprot | |
| S695 | Phosphorylation | Uniprot | |
| S807 | Phosphorylation | Uniprot | |
| T808 | Phosphorylation | Uniprot | |
| S809 | Phosphorylation | Uniprot | |
| S812 | Phosphorylation | Uniprot | |
| T915 | Phosphorylation | Uniprot | |
| S917 | Phosphorylation | Uniprot | |
| S938 | Phosphorylation | Uniprot | |
| T948 | Phosphorylation | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S225 | Phosphorylation | Uniprot | |
| S446 | Phosphorylation | Uniprot | |
| S489 | Phosphorylation | Uniprot | |
| S498 | Phosphorylation | Uniprot | |
| S502 | Phosphorylation | Uniprot | |
| S506 | Phosphorylation | Uniprot | |
| S508 | Phosphorylation | Uniprot | |
| T558 | Phosphorylation | Uniprot | |
| S589 | Phosphorylation | Uniprot | |
| S722 | Phosphorylation | Uniprot | |
| S809 | Phosphorylation | Uniprot | |
| S830 | Phosphorylation | Uniprot |
PTMs - P80192/Q02779 酵素として
研究背景
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade through the phosphorylation of MAP2K4/MKK4 and MAP2K7/MKK7 which in turn activate the JNKs. The MKK/JNK signaling pathway regulates stress response via activator protein-1 (JUN) and GATA4 transcription factors. Plays also a role in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis.
Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-312 is likely to be the main autophosphorylation site. Autophosphorylation also occurs on Thr-304 and Ser-308.
Expressed in epithelial tumor cell lines of colonic, breast and esophageal origin.
Homodimer.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Activates the JUN N-terminal pathway.
Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation.
Expressed in brain and skeletal muscle.
Homodimer. Interacts with SH3RF2.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.