Phospho-hnRNP K (Ser216) Antibody - #AF3639

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製品: Phospho-hnRNP K (Ser216) Antibody
カタログ: AF3639
タンパク質の説明: Rabbit polyclonal antibody to Phospho-hnRNP K (Ser216)
アプリケーション: IF/ICC
反応性: Human, Mouse, Rat
分子量: 51kD(Calculated).
ユニプロット: P61978
RRID: AB_2846953

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製品説明

ソース:
Rabbit
アプリケーション:
IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
Phospho-hnRNP K (Ser216) Antibody detects endogenous levels of hnRNP K only when phosphorylated at Ser216.
RRID:
AB_2846953
引用形式: Affinity Biosciences Cat# AF3639, RRID:AB_2846953.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

CSBP; dC stretch binding protein; FLJ41122; Heterogeneous nuclear ribonucleoprotein K; hnRNP K; HNRNPK; HNRPK; HNRPK_HUMAN; Transformation up regulated nuclear protein; Transformation up-regulated nuclear protein; Transformation upregulated nuclear protein; TUNP;

免疫原

免疫原:

A synthesized peptide derived from human hnRNP K around the phosphorylation site of Ser216.

Uniprot:

P61978(HNRPK_HUMAN) >>Visit HPA database.

遺伝子(ID):
HNRNPK(3190)
タンパク質配列:
METEQPEETFPNTETNGEFGKRPAEDMEEEQAFKRSRNTDEMVELRILLQSKNAGAVIGKGGKNIKALRTDYNASVSVPDSSGPERILSISADIETIGEILKKIIPTLEEGLQLPSPTATSQLPLESDAVECLNYQHYKGSDFDCELRLLIHQSLAGGIIGVKGAKIKELRENTQTTIKLFQECCPHSTDRVVLIGGKPDRVVECIKIILDLISESPIKGRAQPYDPNFYDETYDYGGFTMMFDDRRGRPVGFPMRGRGGFDRMPPGRGGRPMPPSRRDYDDMSPRRGPPPPPPGRGGRGGSRARNLPLPPPPPPRGGDLMAYDRRGRPGDRYDGMVGFSADETWDSAIDTWSPSEWQMAYEPQGGSGYDYSYAGGRGSYGDLGGPIITTQVTIPKDLAGSIIGKGGQRIKQIRHESGASIKIDEPLEGSEDRIITITGTQDQIQNAQYLLQNSVKQYSGKFF

PTMs - P61978 基板として

Site PTM Type Enzyme
M1 Acetylation
T3 Phosphorylation
K21 Acetylation
R22 Methylation
K34 Acetylation
K34 Methylation
K34 Sumoylation
K34 Ubiquitination
S36 Phosphorylation
T39 Phosphorylation
R46 Methylation
K52 Sumoylation
K52 Ubiquitination
K60 Acetylation
K60 Sumoylation
K60 Ubiquitination
K63 Ubiquitination
K66 Ubiquitination
Y72 Phosphorylation P12931 (SRC)
S75 Phosphorylation
S77 Phosphorylation
S81 Phosphorylation
S82 Phosphorylation
S89 Phosphorylation
K102 Ubiquitination
K103 Ubiquitination
S116 Phosphorylation
T118 Phosphorylation
T120 Phosphorylation
S121 Phosphorylation
Y135 Phosphorylation
Y138 Phosphorylation
K139 Methylation
K139 Ubiquitination
S141 Phosphorylation
R148 Methylation
S154 Phosphorylation
K163 Sumoylation
K163 Ubiquitination
K166 Ubiquitination
K168 Ubiquitination
K179 Acetylation
K179 Ubiquitination
C184 S-Nitrosylation
C185 S-Nitrosylation
S188 Phosphorylation
R191 Methylation
K198 Methylation
K198 Ubiquitination
R201 Methylation
K207 Ubiquitination
S214 Phosphorylation
S216 Phosphorylation P53779 (MAPK10) , P06493 (CDK1) , P24941 (CDK2) , P45983 (MAPK8)
K219 Acetylation
K219 Sumoylation
K219 Ubiquitination
Y225 Phosphorylation P12931 (SRC)
Y230 Phosphorylation P12931 (SRC)
Y234 Phosphorylation P12931 (SRC)
Y236 Phosphorylation P12931 (SRC)
R246 Methylation
R247 Methylation
R249 Methylation
R256 Methylation
R258 Methylation
R263 Methylation
R268 Methylation
R271 Methylation
S276 Phosphorylation
R277 Methylation
R278 Methylation
Y280 Phosphorylation
S284 Phosphorylation P28482 (MAPK1) , P27361 (MAPK3)
R286 Methylation
R296 Methylation
R299 Methylation
S302 Phosphorylation Q05655 (PRKCD)
R303 Methylation
R305 Methylation
R316 Methylation
Y323 Phosphorylation
R325 Methylation
R326 Methylation
R328 Methylation
Y333 Phosphorylation
S353 Phosphorylation P53779 (MAPK10) , P45983 (MAPK8) , P27361 (MAPK3)
Y361 Phosphorylation
Y369 Phosphorylation
Y371 Phosphorylation
S372 Phosphorylation
Y373 Phosphorylation
R377 Methylation
S379 Phosphorylation O14965 (AURKA)
Y380 Phosphorylation P12931 (SRC)
T389 Phosphorylation
T390 Phosphorylation
T393 Phosphorylation
K396 Ubiquitination
S401 Phosphorylation
K405 Sumoylation
K405 Ubiquitination
S417 Phosphorylation
S420 Phosphorylation
K422 Acetylation
K422 Sumoylation
K422 Ubiquitination
S430 Phosphorylation
R433 Methylation
T436 Phosphorylation
T440 Phosphorylation
Y449 Phosphorylation
S454 Phosphorylation
K456 Ubiquitination
Y458 Phosphorylation P12931 (SRC)
K461 Acetylation
K461 Ubiquitination

研究背景

機能:

One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction (By similarity). As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest.

PTMs:

Arg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine.

Sumoylated by CBX4. Sumoylation is increased upon DNA damage, such as that produced by doxorubicin, etoposide, UV light and camptothecin, due to enhanced CBX4 phosphorylation by HIPK2 under these conditions.

Ubiquitinated by MDM2. Doxorubicin treatment does not affect monoubiquitination, but slightly decreases HNRNPK poly-ubiquitination.

O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.

細胞の位置付け:

Cytoplasm. Nucleus>Nucleoplasm. Cell projection>Podosome.
Note: Recruited to p53/TP53-responsive promoters, in the presence of functional p53/TP53 (PubMed:16360036). In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear (PubMed:18775702).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Interacts with RBM42 and ZIK1 (By similarity). Interacts with BRDT (By similarity). Identified in the spliceosome C complex. Interacts with ANKRD28. Interacts with ASFV p30 protein. Interacts with DDX1. Interacts with MDM2; this interaction leads to ubiquitination and proteasomal degradation. Interacts with p53/TP53. Interacts with IVNS1ABP.

(Microbial infection) Interacts with HCV core protein.

研究領域

· Genetic Information Processing > Transcription > Spliceosome.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

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