Phospho-TIRAP (Tyr86) Antibody - #AF3647
| 製品: | Phospho-TIRAP (Tyr86) Antibody |
| カタログ: | AF3647 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-TIRAP (Tyr86) |
| アプリケーション: | ELISA(peptide) |
| 反応性: | Human, Mouse |
| 分子量: | 24kD(Calculated). |
| ユニプロット: | P58753 |
| RRID: | AB_2846961 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF3647, RRID:AB_2846961.
折りたたみ／展開
Adapter protein wyatt; FLJ42305; MAL; MyD88 adapter like protein; TIR domain containing adapter protein; Toll interleukin 1 receptor (TIR) domain containing adaptor protein; Toll interleukin 1 receptor domain containing adapter protein; Toll like receptor adaptor protein; Wyatt;
免疫原
A synthesized peptide derived from human TIRAP around the phosphorylation site of Tyr86.
Highly expressed in liver, kidney, spleen, skeletal muscle and heart. Also detected in peripheral blood leukocytes, lung, placenta, small intestine, thymus, colon and brain.
- P58753 TIRAP_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASSTSLPAPGSRPKKPLGKMADWFRQTLLKKPKKRPNSPESTSSDASQPTSQDSPLPPSLSSVTSPSLPPTHASDSGSSRWSKDYDVCVCHSEEDLVAAQDLVSYLEGSTASLRCFLQLRDATPGGAIVSELCQALSSSHCRVLLITPGFLQDPWCKYQMLQALTEAPGAEGCTIPLLSGLSRAAYPPELRFMYYVDGRGPDGGFRQVKEAVMRYLQTLS
PTMs - P58753 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S3 | Phosphorylation | Uniprot | |
| T28 | Phosphorylation | Q9NWZ3 (IRAK4) , P51617 (IRAK1) | Uniprot |
| Y86 | Phosphorylation | Q06187 (BTK) | Uniprot |
| Y106 | Phosphorylation | Q06187 (BTK) | Uniprot |
| T124 | Phosphorylation | Uniprot | |
| Y159 | Phosphorylation | Uniprot | |
| Y187 | Phosphorylation | Q06187 (BTK) | Uniprot |
| Y216 | Phosphorylation | Uniprot | |
| T219 | Phosphorylation | Uniprot | |
| S221 | Phosphorylation | Uniprot |
研究背景
Adapter involved in TLR2 and TLR4 signaling pathways in the innate immune response. Acts via IRAK2 and TRAF-6, leading to the activation of NF-kappa-B, MAPK1, MAPK3 and JNK, and resulting in cytokine secretion and the inflammatory response. Positively regulates the production of TNF-alpha and interleukin-6.
Phosphorylated by IRAK1 and IRAK4. Also phosphorylated by BTK.
Polyubiquitinated. Polyubiquitination follows phosphorylation by BTK and leads to TIRAP degradation.
Cytoplasm. Cell membrane. Membrane.
Note: Colocalizes with DAB2IP at the plasma membrane.
Highly expressed in liver, kidney, spleen, skeletal muscle and heart. Also detected in peripheral blood leukocytes, lung, placenta, small intestine, thymus, colon and brain.
Homodimer. Also forms heterodimers with MYD88. May interact with PIK3AP1 (By similarity). Interacts with TLR4 and IRAK2 via their respective TIR domains. Interacts with BMX and TBK1. Interacts with EIF2AK2. Does not interact with IRAK1, nor TLR9.
(Microbial infection) In case of infection, interacts with Brucella protein BtpA.
研究領域
· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Pertussis.
· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.
· Human Diseases > Infectious diseases: Viral > Hepatitis B.
· Organismal Systems > Immune system > Toll-like receptor signaling pathway. (View pathway)
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