Phospho-MSH6 (Ser14) Antibody | Affinity Biosciences

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AF3652 at 1/100 staining Mouse heart tissue by IHC-P.

製品:	Phospho-MSH6 (Ser14) Antibody
カタログ:	AF3652
タンパク質の説明:	Rabbit polyclonal antibody to Phospho-MSH6 (Ser14)
アプリケーション:	IHC
反応性:	Human, Mouse, Rat
分子量:	153kD(Calculated).
ユニプロット:	P52701
RRID:	AB_2846966

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サイズ	価格	在庫状況
100ul	$350	在庫あり
200ul	$450	在庫あり

プロトコル

製品説明

ソース:

Rabbit

アプリケーション:

IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:

Human,Mouse,Rat

クローナリティ:

Polyclonal

特異性:

Phospho-MSH6 (Ser14) Antibody detects endogenous levels of MSH6 only when phosphorylated at Ser14.

RRID:

AB_2846966
引用形式: Affinity Biosciences Cat# AF3652, RRID:AB_2846966.

コンジュゲート:

Unconjugated.

精製:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

別名:

折りたたみ／展開

DNA mismatch repair protein Msh6; G/T mismatch binding protein; G/T mismatch-binding protein; GTBP; GTMBP; hMSH6; HNPCC 5; HNPCC5; HSAP; MSH 6; MSH6; MSH6_HUMAN; mutS (E. coli) homolog 6; MutS alpha 160 kDa subunit; MutS homolog 6 (E. coli); mutS homolog 6; MutS-alpha 160 kDa subunit; p160; Sperm associated protein;

免疫原

免疫原:

A synthesized peptide derived from human MSH6 around the phosphorylation site of Ser14.

Uniprot:

P52701(MSH6_HUMAN) >>Visit HPA database.

遺伝子(ID):

MSH6(2956)

タンパク質配列:

P52701 MSH6_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSRQSTLYSFFPKSPALSDANKASARASREGGRAAAAPGASPSPGGDAAWSEAGPGPRPLARSASPPKAKNLNGGLRRSVAPAAPTSCDFSPGDLVWAKMEGYPWWPCLVYNHPFDGTFIREKGKSVRVHVQFFDDSPTRGWVSKRLLKPYTGSKSKEAQKGGHFYSAKPEILRAMQRADEALNKDKIKRLELAVCDEPSEPEEEEEMEVGTTYVTDKSEEDNEIESEEEVQPKTQGSRRSSRQIKKRRVISDSESDIGGSDVEFKPDTKEEGSSDEISSGVGDSESEGLNSPVKVARKRKRMVTGNGSLKRKSSRKETPSATKQATSISSETKNTLRAFSAPQNSESQAHVSGGGDDSSRPTVWYHETLEWLKEEKRRDEHRRRPDHPDFDASTLYVPEDFLNSCTPGMRKWWQIKSQNFDLVICYKVGKFYELYHMDALIGVSELGLVFMKGNWAHSGFPEIAFGRYSDSLVQKGYKVARVEQTETPEMMEARCRKMAHISKYDRVVRREICRIITKGTQTYSVLEGDPSENYSKYLLSLKEKEEDSSGHTRAYGVCFVDTSLGKFFIGQFSDDRHCSRFRTLVAHYPPVQVLFEKGNLSKETKTILKSSLSCSLQEGLIPGSQFWDASKTLRTLLEEEYFREKLSDGIGVMLPQVLKGMTSESDSIGLTPGEKSELALSALGGCVFYLKKCLIDQELLSMANFEEYIPLDSDTVSTTRSGAIFTKAYQRMVLDAVTLNNLEIFLNGTNGSTEGTLLERVDTCHTPFGKRLLKQWLCAPLCNHYAINDRLDAIEDLMVVPDKISEVVELLKKLPDLERLLSKIHNVGSPLKSQNHPDSRAIMYEETTYSKKKIIDFLSALEGFKVMCKIIGIMEEVADGFKSKILKQVISLQTKNPEGRFPDLTVELNRWDTAFDHEKARKTGLITPKAGFDSDYDQALADIRENEQSLLEYLEKQRNRIGCRTIVYWGIGRNRYQLEIPENFTTRNLPEEYELKSTKKGCKRYWTKTIEKKLANLINAEERRDVSLKDCMRRLFYNFDKNYKDWQSAVECIAVLDVLLCLANYSRGGDGPMCRPVILLPEDTPPFLELKGSRHPCITKTFFGDDFIPNDILIGCEEEEQENGKAYCVLVTGPNMGGKSTLMRQAGLLAVMAQMGCYVPAEVCRLTPIDRVFTRLGASDRIMSGESTFFVELSETASILMHATAHSLVLVDELGRGTATFDGTAIANAVVKELAETIKCRTLFSTHYHSLVEDYSQNVAVRLGHMACMVENECEDPSQETITFLYKFIKGACPKSYGFNAARLANLPEEVIQKGHRKAREFEKMNQSLRLFREVCLASERSTVDAEAVHKLLTLIKEL

PTMs - P52701 基板として

P52701

Site	PTM Type	Source
Y8	Phosphorylation	Uniprot
S9	Phosphorylation	Uniprot
S14	Phosphorylation	Uniprot
K22	Ubiquitination	Uniprot
R33	Methylation	Uniprot
S41	Phosphorylation	Uniprot
S43	Phosphorylation	Uniprot
S51	Phosphorylation	Uniprot
S63	Phosphorylation	Uniprot
S65	Phosphorylation	Uniprot
K70	Acetylation	Uniprot
S79	Phosphorylation	Uniprot
T86	Phosphorylation	Uniprot
S87	Phosphorylation	Uniprot
S91	Phosphorylation	Uniprot
S137	Phosphorylation	Uniprot
T139	Phosphorylation	Uniprot
R140	Methylation	Uniprot
S144	Phosphorylation	Uniprot
Y151	Phosphorylation	Uniprot
K155	Acetylation	Uniprot
K161	Ubiquitination	Uniprot
K169	Sumoylation	Uniprot
K169	Ubiquitination	Uniprot
S200	Phosphorylation	Uniprot
T212	Phosphorylation	Uniprot
T213	Phosphorylation	Uniprot
Y214	Phosphorylation	Uniprot
T216	Phosphorylation	Uniprot
S219	Phosphorylation	Uniprot
S227	Phosphorylation	Uniprot
T235	Phosphorylation	Uniprot
S252	Phosphorylation	Uniprot
S254	Phosphorylation	Uniprot
S256	Phosphorylation	Uniprot
S261	Phosphorylation	Uniprot
T269	Phosphorylation	Uniprot
S274	Phosphorylation	Uniprot
S275	Phosphorylation	Uniprot
S279	Phosphorylation	Uniprot
S280	Phosphorylation	Uniprot
S285	Phosphorylation	Uniprot
S292	Phosphorylation	Uniprot
T305	Phosphorylation	Uniprot
S309	Phosphorylation	Uniprot
K324	Ubiquitination	Uniprot
K334	Sumoylation	Uniprot
K334	Ubiquitination	Uniprot
S348	Phosphorylation	Uniprot
K374	Ubiquitination	Uniprot
K476	Ubiquitination	Uniprot
K479	Ubiquitination	Uniprot
T488	Phosphorylation	Uniprot
K498	Ubiquitination	Uniprot
K504	Acetylation	Uniprot
K504	Ubiquitination	Uniprot
K519	Ubiquitination	Uniprot
K537	Ubiquitination	Uniprot
K543	Ubiquitination	Uniprot
R577	Methylation	Uniprot
K598	Ubiquitination	Uniprot
K603	Ubiquitination	Uniprot
K610	Ubiquitination	Uniprot
K632	Ubiquitination	Uniprot
Y642	Phosphorylation	Uniprot
K646	Ubiquitination	Uniprot
K660	Ubiquitination	Uniprot
S668	Phosphorylation	Uniprot
K693	Ubiquitination	Uniprot
S702	Phosphorylation	Uniprot
Y709	Phosphorylation	Uniprot
K728	Ubiquitination	Uniprot
T767	Phosphorylation	Uniprot
K771	Ubiquitination	Uniprot
K813	Ubiquitination	Uniprot
K814	Ubiquitination	Uniprot
K824	Ubiquitination	Uniprot
S830	Phosphorylation	Uniprot
K833	Methylation	Uniprot
K833	Ubiquitination	Uniprot
K852	Ubiquitination	Uniprot
K854	Ubiquitination	Uniprot
K866	Ubiquitination	Uniprot
K870	Ubiquitination	Uniprot
K883	Ubiquitination	Uniprot
K888	Ubiquitination	Uniprot
T895	Phosphorylation	Uniprot
K896	Ubiquitination	Uniprot
T914	Phosphorylation	Uniprot
K920	Ubiquitination	Uniprot
K923	Ubiquitination	Uniprot
T924	Phosphorylation	Uniprot
T928	Phosphorylation	Uniprot
K930	Ubiquitination	Uniprot
S935	Phosphorylation	Uniprot
K957	Ubiquitination	Uniprot
K997	Sumoylation	Uniprot
K997	Ubiquitination	Uniprot
K1009	Ubiquitination	Uniprot
T1010	Phosphorylation	Uniprot
K1014	Ubiquitination	Uniprot
K1030	Ubiquitination	Uniprot
T1085	Phosphorylation	Uniprot
K1092	Methylation	Uniprot
K1092	Ubiquitination	Uniprot
K1101	Ubiquitination	Uniprot
K1126	Methylation	Uniprot
K1140	Ubiquitination	Uniprot
R1172	Methylation	Uniprot
K1240	Ubiquitination	Uniprot
K1291	Ubiquitination	Uniprot
K1296	Ubiquitination	Uniprot
K1315	Ubiquitination	Uniprot
K1325	Ubiquitination	Uniprot
K1352	Ubiquitination	Uniprot
K1358	Ubiquitination	Uniprot

研究背景

P52701_MSH6_HUMAN

機能:

Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3 (H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction.

PTMs:

The N-terminus is blocked.

Phosphorylated by PRKCZ, which may prevent MutS alpha degradation by the ubiquitin-proteasome pathway.

細胞の位置付け:

Nucleus. Chromosome.
Note: Associates with H3K36me3 via its PWWP domain.

サブユニット構造:

Component of the DNA mismatch repair (MMR) complex composed at least of MSH2, MSH3, MSH6, PMS1 and MLH1. Heterodimer consisting of MSH2-MSH6 (MutS alpha). Forms a ternary complex with MutL alpha (MLH1-PMS1). Interacts with MCM9. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains.

(Microbial infection) Interacts with herpes simplex virus 1 protein UL12.

タンパク質ファミリー:

The PWWP domain specifically recognizes and binds trimethylated 'Lys-36' of histone H3 (H3K36me3).

Belongs to the DNA mismatch repair MutS family.

研究領域

· Genetic Information Processing > Replication and repair > Mismatch repair.

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Colorectal cancer. (View pathway)

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Phospho-MSH6 (Ser14) Antibody - #AF3652

関連製品情報

関連ダウンロード

MSDS

説明書

COA

プロトコル

WB Handbook

IHC Protocol

IF/ICC Protocol

製品説明

免疫原

PTMs - P52701 基板として

研究背景

研究領域

Restrictive clause