Phospho-STAT2 (Thr387) Antibody - #AF3653
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF3653, RRID:AB_2846967.
折りたたみ／展開
Homo sapiens interferon alpha induced transcriptional activator; interferon alpha induced transcriptional activator; ISGF 3; ISGF3; MGC59816; P113; signal transducer and activator of transcription 2 113kD; Signal transducer and activator of transcription 2; STAT113; Stat2; STAT2_HUMAN;
免疫原
A synthesized peptide derived from human STAT2 around the phosphorylation site of Thr387.
- P52630 STAT2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAQWEMLQNLDSPFQDQLHQLYSHSLLPVDIRQYLAVWIEDQNWQEAALGSDDSKATMLFFHFLDQLNYECGRCSQDPESLLLQHNLRKFCRDIQPFSQDPTQLAEMIFNLLLEEKRILIQAQRAQLEQGEPVLETPVESQQHEIESRILDLRAMMEKLVKSISQLKDQQDVFCFRYKIQAKGKTPSLDPHQTKEQKILQETLNELDKRRKEVLDASKALLGRLTTLIELLLPKLEEWKAQQQKACIRAPIDHGLEQLETWFTAGAKLLFHLRQLLKELKGLSCLVSYQDDPLTKGVDLRNAQVTELLQRLLHRAFVVETQPCMPQTPHRPLILKTGSKFTVRTRLLVRLQEGNESLTVEVSIDRNPPQLQGFRKFNILTSNQKTLTPEKGQSQGLIWDFGYLTLVEQRSGGSGKGSNKGPLGVTEELHIISFTVKYTYQGLKQELKTDTLPVVIISNMNQLSIAWASVLWFNLLSPNLQNQQFFSNPPKAPWSLLGPALSWQFSSYVGRGLNSDQLSMLRNKLFGQNCRTEDPLLSWADFTKRESPPGKLPFWTWLDKILELVHDHLKDLWNDGRIMGFVSRSQERRLLKKTMSGTFLLRFSESSEGGITCSWVEHQDDDKVLIYSVQPYTKEVLQSLPLTEIIRHYQLLTEENIPENPLRFLYPRIPRDEAFGCYYQEKVNLQERRKYLKHRLIVVSNRQVDELQQPLELKPEPELESLELELGLVPEPELSLDLEPLLKAGLDLGPELESVLESTLEPVIEPTLCMVSQTVPEPDQGPVSQPVPEPDLPCDLRHLNTEPMEIFRNCVKIEEIMPNGDPLLAGQNTVDEVYVSRPSHFYTDGPLMPSDF
PTMs - P52630 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S12 | Phosphorylation | Uniprot | |
| T136 | Phosphorylation | Uniprot | |
| K158 | Acetylation | Uniprot | |
| K158 | Ubiquitination | Uniprot | |
| K161 | Ubiquitination | Uniprot | |
| K167 | Ubiquitination | Uniprot | |
| K184 | Ubiquitination | Uniprot | |
| S187 | Phosphorylation | Uniprot | |
| K194 | Ubiquitination | Uniprot | |
| K197 | Ubiquitination | Uniprot | |
| K208 | Ubiquitination | Uniprot | |
| K218 | Ubiquitination | Uniprot | |
| K239 | Ubiquitination | Uniprot | |
| K244 | Ubiquitination | Uniprot | |
| K280 | Ubiquitination | Uniprot | |
| S283 | Phosphorylation | Uniprot | |
| S287 | Phosphorylation | Uniprot | |
| T294 | Phosphorylation | Uniprot | |
| K295 | Ubiquitination | Uniprot | |
| K375 | Acetylation | Uniprot | |
| K375 | Ubiquitination | Uniprot | |
| K384 | Acetylation | Uniprot | |
| K384 | Ubiquitination | Uniprot | |
| T387 | Phosphorylation | Uniprot | |
| K443 | Ubiquitination | Uniprot | |
| K523 | Ubiquitination | Uniprot | |
| K543 | Ubiquitination | Uniprot | |
| K550 | Ubiquitination | Uniprot | |
| S595 | Phosphorylation | Uniprot | |
| T597 | Phosphorylation | Uniprot | |
| Y631 | Phosphorylation | Uniprot | |
| K681 | Ubiquitination | Uniprot | |
| Y690 | Phosphorylation | P06239 (LCK) | Uniprot |
| S699 | Phosphorylation | Uniprot | |
| S734 | Phosphorylation | Uniprot | |
| S753 | Phosphorylation | Uniprot | |
| T800 | Phosphorylation | Uniprot | |
| Y833 | Phosphorylation | Uniprot | |
| S849 | Phosphorylation | Uniprot |
研究背景
Signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. Acts as a regulator of mitochondrial fission by modulating the phosphorylation of DNM1L at 'Ser-616' and 'Ser-637' which activate and inactivate the GTPase activity of DNM1L respectively.
Tyrosine phosphorylated in response to IFN-alpha. Phosphorylation at Ser-287 negatively regulates the transcriptional response.
'Lys-48'-linked ubiquitination by DCST1 leads to STAT2 proteasomal degradation.
Cytoplasm. Nucleus.
Note: Translocated into the nucleus upon activation by IFN-alpha/beta.
Heterodimer with STAT1 upon IFN-alpha/beta induced phosphorylation (By similarity). The heterodimer STAT1:STAT2 forms the interferon-stimulated gene factor 3 complex (ISGF3) with IRF9; interacts with IRF9 in the cytoplasm (By similarity). Interacts with CRSP2 and CRSP6. Can form a homodimer upon IFN-alpha induced phosphorylation. Interacts with IFNAR1; the interaction requires the phosphorylation of IFNAR1 at 'Tyr-466'. Interacts with IFNAR2. Interacts with ARL2BP (By similarity). Interacts with E3 ubiquitin ligase DCST1; the interaction results in STAT2 ubiquitin-mediated proteasomal degradation.
(Microbial infection) Interacts with vaccinia virus protein C6.
(Microbial infection) Interacts with Simian virus 5 protein V.
(Microbial infection) Interacts with Rabies virus phosphoprotein.
(Microbial infection) Interacts with Human cytomegalovirus/HHV-5 protein UL123; this interaction promotes viral growth.
(Microbial infection) Interacts with Dengue virus NS5; this interaction inhibits the phosphorylation of STAT2, and, when all viral proteins are present (polyprotein), targets STAT2 for degradation.
Belongs to the transcription factor STAT family.
研究領域
· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)
· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Viral > Hepatitis C.
· Human Diseases > Infectious diseases: Viral > Hepatitis B.
· Human Diseases > Infectious diseases: Viral > Measles.
· Human Diseases > Infectious diseases: Viral > Influenza A.
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)
· Organismal Systems > Development > Osteoclast differentiation. (View pathway)
· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)
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