Phospho-HNF4 alpha (Ser304) Antibody - #AF3671
| 製品: | Phospho-HNF4 alpha (Ser304) Antibody |
| カタログ: | AF3671 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-HNF4 alpha (Ser304) |
| アプリケーション: | IHC |
| 反応性: | Human, Mouse, Rat |
| 分子量: | 53kD(Calculated). |
| ユニプロット: | P41235 |
| RRID: | AB_2846985 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF3671, RRID:AB_2846985.
折りたたみ／展開
FLJ39654; FRTS4; Hepatic nuclear factor 4 alpha; Hepatocyte nuclear factor 4 alpha; Hepatocyte nuclear factor 4; Hepatocyte nuclear factor 4-alpha; HNF 4 alpha; HNF 4; HNF-4-alpha; HNF4; HNF4A; HNF4A_HUMAN; HNF4a7; HNF4a8; HNF4a9; Hnf4alpha; HNF4alpha10/11/12; MODY 1; MODY; MODY1; NR2A1; NR2A21; Nuclear receptor subfamily 2 group A member 1; OTTHUMP00000031060; OTTHUMP00000031062; TCF 14; TCF; TCF-14; TCF14; Tcf4; Transcription factor 14, hepatic nuclear factor; Transcription factor 14; Transcription factor HNF 4; Transcription factor HNF-4; Transcription factor HNF4;
免疫原
A synthesized peptide derived from human HNF4 alpha around the phosphorylation site of Ser304.
- P41235 HNF4A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRLSKTLVDMDMADYSAALDPAYTTLEFENVQVLTMGNDTSPSEGTNLNAPNSLGVSALCAICGDRATGKHYGASSCDGCKGFFRRSVRKNHMYSCRFSRQCVVDKDKRNQCRYCRLKKCFRAGMKKEAVQNERDRISTRRSSYEDSSLPSINALLQAEVLSRQITSPVSGINGDIRAKKIASIADVCESMKEQLLVLVEWAKYIPAFCELPLDDQVALLRAHAGEHLLLGATKRSMVFKDVLLLGNDYIVPRHCPELAEMSRVSIRILDELVLPFQELQIDDNEYAYLKAIIFFDPDAKGLSDPGKIKRLRSQVQVSLEDYINDRQYDSRGRFGELLLLLPTLQSITWQMIEQIQFIKLFGMAKIDNLLQEMLLGGSPSDAPHAHHPLHPHLMQEHMGTNVIVANTMPTHLSNGQMCEWPRPRGQAATPETPQPSPPGGSGSEPYKLLPGAVATIVKPLSAIPQPTITKQEVI
PTMs - P41235 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y23 | Phosphorylation | P12931 (SRC) | Uniprot |
| Y72 | Phosphorylation | Uniprot | |
| S75 | Phosphorylation | Uniprot | |
| S76 | Phosphorylation | Uniprot | |
| S95 | Phosphorylation | Uniprot | |
| S99 | Phosphorylation | Uniprot | |
| K106 | Acetylation | Uniprot | |
| K108 | Acetylation | Uniprot | |
| K126 | Acetylation | Uniprot | |
| K127 | Acetylation | Uniprot | |
| S138 | Phosphorylation | Uniprot | |
| T139 | Phosphorylation | Uniprot | |
| S142 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S143 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| Y144 | Phosphorylation | Uniprot | |
| S147 | Phosphorylation | Uniprot | |
| S148 | Phosphorylation | Uniprot | |
| S151 | Phosphorylation | Uniprot | |
| T166 | Phosphorylation | Uniprot | |
| S167 | Phosphorylation | Q16539 (MAPK14) , Q15759 (MAPK11) | Uniprot |
| S170 | Phosphorylation | Uniprot | |
| S190 | Phosphorylation | Uniprot | |
| K234 | Ubiquitination | Uniprot | |
| S262 | Phosphorylation | Uniprot | |
| S265 | Phosphorylation | Uniprot | |
| Y286 | Phosphorylation | P12931 (SRC) | Uniprot |
| Y288 | Phosphorylation | P12931 (SRC) | Uniprot |
| S303 | Phosphorylation | Q13131 (PRKAA1) | Uniprot |
| K307 | Ubiquitination | Uniprot | |
| K309 | Ubiquitination | Uniprot | |
| S313 | Phosphorylation | Q13131 (PRKAA1) | Uniprot |
| S318 | Phosphorylation | Uniprot | |
| S378 | Phosphorylation | Uniprot | |
| T429 | Phosphorylation | Uniprot | |
| T432 | Phosphorylation | Uniprot | |
| S436 | Phosphorylation | Uniprot | |
| S441 | Phosphorylation | Uniprot | |
| S443 | Phosphorylation | Uniprot | |
| T455 | Phosphorylation | Uniprot | |
| K458 | Acetylation | Uniprot | |
| S461 | Phosphorylation | Uniprot | |
| T467 | Phosphorylation | Uniprot | |
| T469 | Phosphorylation | Uniprot |
研究背景
Transcriptional regulator which controls the expression of hepatic genes during the transition of endodermal cells to hepatic progenitor cells, facilitating the recruitment of RNA pol II to the promoters of target genes. Activates the transcription of CYP2C38 (By similarity). Represses the CLOCK-ARNTL/BMAL1 transcriptional activity and is essential for circadian rhythm maintenance and period regulation in the liver and colon cells.
Phosphorylated on tyrosine residue(s); phosphorylation is important for its DNA-binding activity. Phosphorylation may directly or indirectly play a regulatory role in the subnuclear distribution. Phosphorylation at Ser-313 by AMPK reduces the ability to form homodimers and bind DNA.
Acetylation at Lys-458 lowers transcriptional activation by about two-fold.
Nucleus.
Homodimerization is required for HNF4-alpha to bind to its recognition site. Interacts with CLOCK, ARNTL, CRY1, CRY2, PER1 and PER2. Interacts with NR0B2/SHP; the resulting heterodimer is transcriptionnally inactive. Interacts with DDX3X; this interaction disrupts the interaction between HNF4 and NR0B2 that forms inactive heterodimers and enhances the formation of active HNF4 homodimers.
The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
Belongs to the nuclear hormone receptor family. NR2 subfamily.
研究領域
· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)
· Human Diseases > Endocrine and metabolic diseases > Maturity onset diabetes of the young.
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