Phospho-Serotonin transporter (Thr276) Antibody - #AF3684
| 製品: | Phospho-Serotonin transporter (Thr276) Antibody |
| カタログ: | AF3684 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-Serotonin transporter (Thr276) |
| アプリケーション: | ELISA(peptide) |
| 反応性: | Human, Mouse, Rat |
| 分子量: | 70kD(Calculated). |
| ユニプロット: | P31645 |
| RRID: | AB_2846998 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF3684, RRID:AB_2846998.
折りたたみ／展開
5 HTT; 5 HTTLPR; 5 hydroxytryptamine (serotonin) transporter; 5 hydroxytryptamine transporter; 5HT transporter; 5HTT; hSERT; HTT; Na+/Cl- dependent serotonin transporter; OCD1; SC6A4_HUMAN; Serotonin transporter 1; SERT; SERT1; Slc6a4; Sodium dependent serotonin transporter; Sodium-dependent serotonin transporter; Solute carrier family 6 (neurotransmitter transporter) member 4; solute carrier family 6 (neurotransmitter transporter, serotonin), member 4; Solute carrier family 6 member 4;
免疫原
A synthesized peptide derived from human Serotonin transporter around the phosphorylation site of Thr276.
- P31645 SC6A4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
METTPLNSQKQLSACEDGEDCQENGVLQKVVPTPGDKVESGQISNGYSAVPSPGAGDDTRHSIPATTTTLVAELHQGERETWGKKVDFLLSVIGYAVDLGNVWRFPYICYQNGGGAFLLPYTIMAIFGGIPLFYMELALGQYHRNGCISIWRKICPIFKGIGYAICIIAFYIASYYNTIMAWALYYLISSFTDQLPWTSCKNSWNTGNCTNYFSEDNITWTLHSTSPAEEFYTRHVLQIHRSKGLQDLGGISWQLALCIMLIFTVIYFSIWKGVKTSGKVVWVTATFPYIILSVLLVRGATLPGAWRGVLFYLKPNWQKLLETGVWIDAAAQIFFSLGPGFGVLLAFASYNKFNNNCYQDALVTSVVNCMTSFVSGFVIFTVLGYMAEMRNEDVSEVAKDAGPSLLFITYAEAIANMPASTFFAIIFFLMLITLGLDSTFAGLEGVITAVLDEFPHVWAKRRERFVLAVVITCFFGSLVTLTFGGAYVVKLLEEYATGPAVLTVALIEAVAVSWFYGITQFCRDVKEMLGFSPGWFWRICWVAISPLFLLFIICSFLMSPPQLRLFQYNYPYWSIILGYCIGTSSFICIPTYIAYRLIITPGTFKERIIKSITPETPTEIPCGDIRLNAV
PTMs - P31645 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S13 | Phosphorylation | Q9UQM7 (CAMK2A) | Uniprot |
| Y47 | Phosphorylation | Uniprot | |
| T59 | Phosphorylation | Uniprot | |
| S62 | Phosphorylation | Uniprot | |
| T81 | Phosphorylation | Uniprot | |
| Y142 | Phosphorylation | Uniprot | |
| S149 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| N208 | N-Glycosylation | Uniprot | |
| N217 | N-Glycosylation | Uniprot | |
| T276 | Phosphorylation | Q13976 (PRKG1) | Uniprot |
| S277 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| T603 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| S611 | Phosphorylation | Uniprot | |
| T613 | Phosphorylation | Uniprot | |
| T616 | Phosphorylation | P28482 (MAPK1) , P45984 (MAPK9) , P27361 (MAPK3) , Q16539 (MAPK14) | Uniprot |
研究背景
Serotonin transporter whose primary function in the central nervous system involves the regulation of serotonergic signaling via transport of serotonin molecules from the synaptic cleft back into the pre-synaptic terminal for re-utilization. Plays a key role in mediating regulation of the availability of serotonin to other receptors of serotonergic systems. Terminates the action of serotonin and recycles it in a sodium-dependent manner.
Glycosylated; modification with sialylated N-glycans is a requirement for transporters to associate with each other and to function as homooligomeric forms.
Phosphorylation at Thr-276 increases 5-HT uptake and is required for cGMP-mediated SERT regulation. Phosphorylation upon PKC stimulation modifies the SERT distribution and density in the membrane, and diminishes the uptake capacity.
Cell membrane>Multi-pass membrane protein. Endomembrane system>Multi-pass membrane protein. Endosome membrane>Multi-pass membrane protein. Cell junction>Synapse. Cell junction>Focal adhesion.
Note: Could be part of recycling endosomes (PubMed:18227069, PubMed:16870614). Density of transporter molecules on the plasma membrane is itself regulated by STX1A (By similarity). Density of transporter molecules on the plasma membrane is also regulated by serotonin (PubMed:17506858) (PubMed:18227069). Density of transporter molecules seems to be modulated by ITGAV:ITGB3 (By similarity).
Expressed in platelets (at protein level).
Monomer or homooligomer (By similarity). Interacts with TGFB1I1. Interacts (via sialylated form) with MYH9. Interacts with SEC23A, SEC24C and PATJ. Interacts with NOS1; the interaction may diminish the cell surface localization of SERT in the brain and, correspondingly, reduce serotonin reuptake. Interacts with filamentous actin and STX1A (By similarity). Interacts (via C-terminus) with VIM. Interacts (via C-terminus) with SCAMP2; the interaction is direct and retains transporter molecules intracellularly. Interacts with RAB4 (GTP-bound form); the interaction retains transporter molecules intracellularly. Interacts with ITGAV:ITGB3 (By similarity).
Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A4 subfamily.
研究領域
· Organismal Systems > Nervous system > Serotonergic synapse.
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