製品: Phospho-MSP/MST1 (Thr353) Antibody
カタログ: AF3688
タンパク質の説明: Rabbit polyclonal antibody to Phospho-MSP/MST1 (Thr353)
アプリケーション: WB
反応性: Human, Mouse, Rat
分子量: 85kD; 80kD(Calculated).
ユニプロット: P26927
RRID: AB_2847002

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
Phospho-MST1 (Thr353) Antibody detects endogenous levels of MST1 only when phosphorylated at Thr353.
RRID:
AB_2847002
引用形式: Affinity Biosciences Cat# AF3688, RRID:AB_2847002.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

D3F15S2; DNF15S2; Hepatocyte growth factor like protein alpha chain; Hepatocyte growth factor like protein; Hepatocyte growth factor like protein beta chain; Hepatocyte growth factor like protein homolog; Hepatocyte growth factor-like protein beta chain; HGFL; HGFL_HUMAN; Macrophage stimulating 1 (hepatocyte growth factor like); Macrophage stimulatory protein; Macrophage-stimulating protein; MSP; MST1; NF15S2; OTTHUMP00000208927;

免疫原

免疫原:

A synthesized peptide derived from human MST1 around the phosphorylation site of Thr353.

Uniprot:
遺伝子(ID):
タンパク質配列:
MGWLPLLLLLTQCLGVPGQRSPLNDFQVLRGTELQHLLHAVVPGPWQEDVADAEECAGRCGPLMDCRAFHYNVSSHGCQLLPWTQHSPHTRLRRSGRCDLFQKKDYVRTCIMNNGVGYRGTMATTVGGLPCQAWSHKFPNDHKYTPTLRNGLEENFCRNPDGDPGGPWCYTTDPAVRFQSCGIKSCREAACVWCNGEEYRGAVDRTESGRECQRWDLQHPHQHPFEPGKFLDQGLDDNYCRNPDGSERPWCYTTDPQIEREFCDLPRCGSEAQPRQEATTVSCFRGKGEGYRGTANTTTAGVPCQRWDAQIPHQHRFTPEKYACKDLRENFCRNPDGSEAPWCFTLRPGMRAAFCYQIRRCTDDVRPQDCYHGAGEQYRGTVSKTRKGVQCQRWSAETPHKPQFTFTSEPHAQLEENFCRNPDGDSHGPWCYTMDPRTPFDYCALRRCADDQPPSILDPPDQVQFEKCGKRVDRLDQRRSKLRVVGGHPGNSPWTVSLRNRQGQHFCGGSLVKEQWILTARQCFSSCHMPLTGYEVWLGTLFQNPQHGEPSLQRVPVAKMVCGPSGSQLVLLKLERSVTLNQRVALICLPPEWYVVPPGTKCEIAGWGETKGTGNDTVLNVALLNVISNQECNIKHRGRVRESEMCTEGLLAPVGACEGDYGGPLACFTHNCWVLEGIIIPNRVCARSRWPAVFTRVSVFVDWIHKVMRLG

PTMs - P26927 基板として

Site PTM Type Enzyme
S185 Phosphorylation
Y199 Phosphorylation
N296 N-Glycosylation
S480 Phosphorylation
K573 Ubiquitination
N615 N-Glycosylation

PTMs - P26927 酵素として

Substrate Site Source
P33778 (HIST1H2BB) S15 Uniprot
Q13043 (STK4) T183 Uniprot
Q15208 (STK38) T444 Uniprot

研究背景

PTMs:

Cleaved after Arg-483, probably by HPN/Hepsin, to yield the active form consisting of two disulfide-linked chains.

細胞の位置付け:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Dimer of an alpha chain and a beta chain linked by a disulfide bond. Interacts (via beta chain) with MST1R (via SEMA domain).

タンパク質ファミリー:

Belongs to the peptidase S1 family. Plasminogen subfamily.

参考文献

1). Mechanical stretching can modify the papillary dermis pattern and papillary fibroblast characteristics during skin regeneration. Journal of Investigative Dermatology, 2022 (PubMed: 35181299) [IF=6.5]

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