製品: Phospho-YY1 (Ser365) Antibody
カタログ: AF3694
タンパク質の説明: Rabbit polyclonal antibody to Phospho-YY1 (Ser365)
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
分子量: 45kD(Calculated).
ユニプロット: P25490
RRID: AB_2847008

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製品説明

ソース:
Rabbit
アプリケーション:
IHC 1:50-1:200, IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
Phospho-YY1 (Ser365) Antibody detects endogenous levels of YY1 only when phosphorylated at Ser365.
RRID:
AB_2847008
引用形式: Affinity Biosciences Cat# AF3694, RRID:AB_2847008.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

CF1; Delta; Delta transcription factor; INO80 complex subunit S; INO80S; NF E1; NF-E1; NFE1; OTTHUMP00000197459; Transcriptional repressor protein YY1; TYY1_HUMAN; UCR motif DNA binding protein; UCRBP; Yin and yang 1; Yin and Yang 1 protein; Yin Yang 1; Ying Yang 1; YY 1; YY 1 transcription factor; YY-1; YY1; YY1 transcription factor;

免疫原

免疫原:

A synthesized peptide derived from human YY1 around the phosphorylation site of Ser365.

Uniprot:
遺伝子(ID):
タンパク質配列:
MASGDTLYIATDGSEMPAEIVELHEIEVETIPVETIETTVVGEEEEEDDDDEDGGGGDHGGGGGHGHAGHHHHHHHHHHHPPMIALQPLVTDDPTQVHHHQEVILVQTREEVVGGDDSDGLRAEDGFEDQILIPVPAPAGGDDDYIEQTLVTVAAAGKSGGGGSSSSGGGRVKKGGGKKSGKKSYLSGGAGAAGGGGADPGNKKWEQKQVQIKTLEGEFSVTMWSSDEKKDIDHETVVEEQIIGENSPPDYSEYMTGKKLPPGGIPGIDLSDPKQLAEFARMKPRKIKEDDAPRTIACPHKGCTKMFRDNSAMRKHLHTHGPRVHVCAECGKAFVESSKLKRHQLVHTGEKPFQCTFEGCGKRFSLDFNLRTHVRIHTGDRPYVCPFDGCNKKFAQSTNLKSHILTHAKAKNNQ

PTMs - P25490 基板として

Site PTM Type Enzyme
Y8 Phosphorylation P07948 (LYN)
T39 Phosphorylation P53350 (PLK1)
S118 Phosphorylation P68400 (CSNK2A1)
S165 Phosphorylation
K173 Ubiquitination
K174 Acetylation
S180 Phosphorylation Q96GD4 (AURKB)
K183 Methylation
K183 Sumoylation
K183 Ubiquitination
S184 Phosphorylation Q96GD4 (AURKB)
Y185 Phosphorylation
S187 Phosphorylation
K203 Acetylation
K203 Sumoylation
K203 Ubiquitination
K204 Acetylation
K208 Ubiquitination
S225 Phosphorylation
S226 Phosphorylation
S247 Phosphorylation
Y251 Phosphorylation
Y254 Phosphorylation P07948 (LYN)
K258 Acetylation
K258 Ubiquitination
K274 Ubiquitination
K286 Sumoylation
K288 Sumoylation
K288 Ubiquitination
K301 Methylation
K301 Ubiquitination
K305 Methylation
K305 Ubiquitination
R308 Methylation
S311 Phosphorylation
K315 Methylation
K315 Ubiquitination
K332 Ubiquitination
K339 Acetylation
K339 Sumoylation
K339 Ubiquitination
T348 Phosphorylation
K351 Acetylation
K351 Sumoylation
K351 Ubiquitination
K362 Methylation
S365 Phosphorylation
T378 Phosphorylation
R381 Methylation
Y383 Phosphorylation P07948 (LYN)
K393 Sumoylation
K393 Ubiquitination
K401 Sumoylation
K401 Ubiquitination
K409 Ubiquitination

研究背景

機能:

Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. Binds to the consensus sequence 5'-CCGCCATNTT-3'; some genes have been shown to contain a longer binding motif allowing enhanced binding; the initial CG dinucleotide can be methylated greatly reducing the binding affinity. The effect on transcription regulation is depending upon the context in which it binds and diverse mechanisms of action include direct activation or repression, indirect activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes. Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression. For example, it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence. Acts synergistically with the SMAD1 and SMAD4 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions. May play an important role in development and differentiation. Proposed to recruit the PRC2/EED-EZH2 complex to target genes that are transcriptional repressed. Involved in DNA repair. In vitro, binds to DNA recombination intermediate structures (Holliday junctions). Plays a role in regulating enhancer activation.

Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair; proposed to target the INO80 complex to YY1-responsive elements.

PTMs:

Transiently poly-ADP-ribosylated by PARP1 upon DNA damage, with the effect of decreasing affinity of YY1 to its cognate DNA binding sites.

Ubiquitinated.

細胞の位置付け:

Nucleus matrix.
Note: Associated with the nuclear matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Interacts with YAF2 through the region encompassing the first and second zinc fingers. Component of the chromatin remodeling INO80 complex; specifically part of a complex module associated with the DBINO domain of INO80. Interacts with EED and EZH2; the interactions are indicative for an association with the PRC2/EED-EZH2 complex. Interacts with SFMBT2. Found in a complex with SMAD1 and SMAD4. Found in a complex with YY1, SIN3A and HDAC1 (By similarity).

タンパク質ファミリー:

Belongs to the YY transcription factor family.

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