Phospho-JAK1 (Tyr1034/Tyr1035) Antibody - #AF3701

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製品: Phospho-JAK1 (Tyr1034/Tyr1035) Antibody
カタログ: AF3701
タンパク質の説明: Rabbit polyclonal antibody to Phospho-JAK1 (Tyr1034/Tyr1035)
アプリケーション: WB
反応性: Human, Mouse, Rat
分子量: 133kD(Calculated).
ユニプロット: P23458
RRID: AB_2847015

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
Phospho-JAK1 (Tyr1034/Tyr1035) Antibody detects endogenous levels of JAK1 only when phosphorylated at Tyr1034/Tyr1035.
RRID:
AB_2847015
引用形式: Affinity Biosciences Cat# AF3701, RRID:AB_2847015.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

JAK 1; JAK 1A; JAK 1B; JAK-1; JAK1; JAK1_HUMAN; JAK1A; JAK1B; Janus kinase 1 (a protein tyrosine kinase); Janus kinase 1; JTK3; Tyrosine protein kinase JAK 1; Tyrosine protein kinase JAK1; Tyrosine-protein kinase JAK1;

免疫原

免疫原:

A synthesized peptide derived from human JAK1 around the phosphorylation site of Tyr1034/Tyr1035.

Uniprot:

P23458(JAK1_HUMAN) >>Visit HPA database.

遺伝子(ID):
JAK1(3716)
発現特異性:
P23458 JAK1_HUMAN:

Expressed at higher levels in primary colon tumors than in normal colon tissue. The expression level in metastatic colon tumors is comparable to the expression level in normal colon tissue.

タンパク質配列:
MQYLNIKEDCNAMAFCAKMRSSKKTEVNLEAPEPGVEVIFYLSDREPLRLGSGEYTAEELCIRAAQACRISPLCHNLFALYDENTKLWYAPNRTITVDDKMSLRLHYRMRFYFTNWHGTNDNEQSVWRHSPKKQKNGYEKKKIPDATPLLDASSLEYLFAQGQYDLVKCLAPIRDPKTEQDGHDIENECLGMAVLAISHYAMMKKMQLPELPKDISYKRYIPETLNKSIRQRNLLTRMRINNVFKDFLKEFNNKTICDSSVSTHDLKVKYLATLETLTKHYGAEIFETSMLLISSENEMNWFHSNDGGNVLYYEVMVTGNLGIQWRHKPNVVSVEKEKNKLKRKKLENKHKKDEEKNKIREEWNNFSYFPEITHIVIKESVVSINKQDNKKMELKLSSHEEALSFVSLVDGYFRLTADAHHYLCTDVAPPLIVHNIQNGCHGPICTEYAINKLRQEGSEEGMYVLRWSCTDFDNILMTVTCFEKSEQVQGAQKQFKNFQIEVQKGRYSLHGSDRSFPSLGDLMSHLKKQILRTDNISFMLKRCCQPKPREISNLLVATKKAQEWQPVYPMSQLSFDRILKKDLVQGEHLGRGTRTHIYSGTLMDYKDDEGTSEEKKIKVILKVLDPSHRDISLAFFEAASMMRQVSHKHIVYLYGVCVRDVENIMVEEFVEGGPLDLFMHRKSDVLTTPWKFKVAKQLASALSYLEDKDLVHGNVCTKNLLLAREGIDSECGPFIKLSDPGIPITVLSRQECIERIPWIAPECVEDSKNLSVAADKWSFGTTLWEICYNGEIPLKDKTLIEKERFYESRCRPVTPSCKELADLMTRCMNYDPNQRPFFRAIMRDINKLEEQNPDIVSEKKPATEVDPTHFEKRFLKRIRDLGEGHFGKVELCRYDPEGDNTGEQVAVKSLKPESGGNHIADLKKEIEILRNLYHENIVKYKGICTEDGGNGIKLIMEFLPSGSLKEYLPKNKNKINLKQQLKYAVQICKGMDYLGSRQYVHRDLAARNVLVESEHQVKIGDFGLTKAIETDKEYYTVKDDRDSPVFWYAPECLMQSKFYIASDVWSFGVTLHELLTYCDSDSSPMALFLKMIGPTHGQMTVTRLVNTLKEGKRLPCPPNCPDEVYQLMRKCWEFQPSNRTSFQNLIEGFEALLK

PTMs - P23458 基板として

Site PTM Type Enzyme
Phosphorylation
M1 Acetylation
Y3 Phosphorylation
K100 Ubiquitination
T119 Phosphorylation
S125 Phosphorylation
K142 Ubiquitination
K213 Ubiquitination
S216 Phosphorylation
Y217 Phosphorylation
Y220 Phosphorylation
K227 Ubiquitination
S228 Phosphorylation
K245 Ubiquitination
K249 Ubiquitination
K254 Ubiquitination
K267 Ubiquitination
K269 Acetylation
K269 Ubiquitination
S333 Phosphorylation
K386 Ubiquitination
Y412 Phosphorylation
K493 Ubiquitination
K496 Ubiquitination
K504 Ubiquitination
Y507 Phosphorylation
S515 Phosphorylation
S518 Phosphorylation
S524 Phosphorylation
K541 Ubiquitination
K559 Ubiquitination
Y568 Phosphorylation
S574 Phosphorylation
K581 Ubiquitination
Y605 Phosphorylation
K606 Ubiquitination
T687 Phosphorylation
T688 Phosphorylation
K691 Ubiquitination
K696 Ubiquitination
K708 Ubiquitination
K718 Ubiquitination
K736 Ubiquitination
S738 Phosphorylation
K768 Ubiquitination
K802 Ubiquitination
K847 Ubiquitination
S857 Phosphorylation
K860 Ubiquitination
K872 Ubiquitination
K888 Ubiquitination
K908 Ubiquitination
S909 Phosphorylation
K911 Ubiquitination
K923 Ubiquitination
K924 Ubiquitination
K939 Ubiquitination
K941 Ubiquitination
Y967 Phosphorylation
K978 Ubiquitination
Y993 Phosphorylation
K1018 Ubiquitination
K1026 Ubiquitination
T1030 Phosphorylation
K1032 Ubiquitination
Y1034 Phosphorylation P52333 (JAK3) , P23458 (JAK1)
Y1035 Phosphorylation P23458 (JAK1) , P52333 (JAK3)
T1036 Phosphorylation
K1038 Ubiquitination
S1043 Phosphorylation
T1107 Phosphorylation
K1109 Acetylation
K1109 Ubiquitination
K1112 Acetylation
Y1125 Phosphorylation
K1130 Ubiquitination

PTMs - P23458 酵素として

Substrate Site Source
P14784 (IL2RB) Y364 Uniprot
P15260 (IFNGR1) Y457 Uniprot
P19525-1 (EIF2AK2) Y101 Uniprot
P19525-1 (EIF2AK2) Y293 Uniprot
P23458 (JAK1) Y1034 Uniprot
P23458 (JAK1) Y1035 Uniprot
P29597 (TYK2) Y1054 Uniprot
P29597 (TYK2) Y1055 Uniprot
P35568 (IRS1) Y612 Uniprot
P35568 (IRS1) Y632 Uniprot
P35568 (IRS1) Y662 Uniprot
P35568 (IRS1) Y732 Uniprot
P40763-2 (STAT3) Y704 Uniprot
P40763 (STAT3) Y705 Uniprot
P42224-2 (STAT1) Y701 Uniprot
P52630-3 (STAT2) Y690 Uniprot
Q13651 (IL10RA) Y446 Uniprot
Q13651 (IL10RA) Y496 Uniprot
Q99683 (MAP3K5) Y718 Uniprot

研究背景

機能:

Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor as well as interleukin (IL)-10 receptor.

PTMs:

Autophosphorylated. Phosphorylated on tyrosine residues in response to interferon gamma signaling. Dephosphorylation of Tyr-1034 and Tyr-1035 by PTPN2 negatively regulates cytokine-mediated signaling.

Ubiquitinated by RNF125; leading to its degradation by the proteasome.

細胞の位置付け:

Endomembrane system>Peripheral membrane protein.
Note: Wholly intracellular, possibly membrane associated.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed at higher levels in primary colon tumors than in normal colon tissue. The expression level in metastatic colon tumors is comparable to the expression level in normal colon tissue.

サブユニット構造:

Interacts with IL31RA. Interacts with IFNAR2. Interacts with IFNGR1. Interacts with JAKMIP1. Interacts with SHB. Interacts (via N-terminus) with IL2RB and IL10RA (via its cytoplasmic domain). Interacts with FER (By similarity).

タンパク質ファミリー:

Possesses two phosphotransferase domains. The second one probably contains the catalytic domain, while the presence of slight differences suggest a different role for domain 1.

The FERM domain mediates interaction with JAKMIP1.

Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.

研究領域

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

参考文献

1). DCUN1D1, a new molecule involved in depigmentation via upregulating CXCL10. Experimental dermatology, 2023 (PubMed: 36541112) [IF=3.6]

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