製品: Phospho-IRF2 (Ser225) Antibody
カタログ: AF3723
タンパク質の説明: Rabbit polyclonal antibody to Phospho-IRF2 (Ser225)
アプリケーション: ELISA(peptide)
反応性: Human, Mouse
分子量: 39kD(Calculated).
ユニプロット: P14316
RRID: AB_2847037

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製品説明

ソース:
Rabbit
アプリケーション:
ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
クローナリティ:
Polyclonal
特異性:
Phospho-IRF2 (Ser225) Antibody detects endogenous levels of IRF2 only when phosphorylated at Ser225.
RRID:
AB_2847037
引用形式: Affinity Biosciences Cat# AF3723, RRID:AB_2847037.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

DKFZp686F0244; Interferon regulatory factor 2; IRF 2; IRF-2; IRF2; IRF2_HUMAN;

免疫原

免疫原:

A synthesized peptide derived from human IRF2 around the phosphorylation site of Ser225.

Uniprot:
遺伝子(ID):
発現特異性:
P14316 IRF2_HUMAN:

Expressed throughout the epithelium of the colon. Also expressed in lamina propria.

タンパク質配列:
MPVERMRMRPWLEEQINSNTIPGLKWLNKEKKIFQIPWMHAARHGWDVEKDAPLFRNWAIHTGKHQPGVDKPDPKTWKANFRCAMNSLPDIEEVKDKSIKKGNNAFRVYRMLPLSERPSKKGKKPKTEKEDKVKHIKQEPVESSLGLSNGVSDLSPEYAVLTSTIKNEVDSTVNIIVVGQSHLDSNIENQEIVTNPPDICQVVEVTTESDEQPVSMSELYPLQISPVSSYAESETTDSVPSDEESAEGRPHWRKRNIEGKQYLSNMGTRGSYLLPGMASFVTSNKPDLQVTIKEESNPVPYNSSWPPFQDLPLSSSMTPASSSSRPDRETRASVIKKTSDITQARVKSC

PTMs - P14316 基板として

Site PTM Type Enzyme
K29 Acetylation
K31 Acetylation
K32 Acetylation
K75 Acetylation
K78 Acetylation
K78 Sumoylation
K78 Ubiquitination
S119 Phosphorylation
K137 Sumoylation
S143 Phosphorylation
S144 Phosphorylation
S148 Phosphorylation
T162 Phosphorylation
S163 Phosphorylation
T164 Phosphorylation
K166 Sumoylation
S225 Phosphorylation
S241 Phosphorylation
K260 Sumoylation
K293 Sumoylation
S333 Phosphorylation

研究背景

機能:

Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and represses those genes. Also acts as an activator for several genes including H4 and IL7. Constitutively binds to the ISRE promoter to activate IL7. Involved in cell cycle regulation through binding the site II (HiNF-M) promoter region of H4 and activating transcription during cell growth. Antagonizes IRF1 transcriptional activation.

PTMs:

Acetylated by CBP/ p300 during cell-growth. Acetylation on Lys-75 is required for stimulation of H4 promoter activity.

The major sites of sumoylation are Lys-137 and Lys-293. Sumoylation with SUMO1 increases its transcriptional repressor activity on IRF1 and diminishes its ability to activate ISRE and H4 promoter.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed throughout the epithelium of the colon. Also expressed in lamina propria.

サブユニット構造:

Interacts with BRD7, IRF2BP1 and IRF2BP2. Interacts with CREBBP in growing cells; the interaction acetylates IRF2 and regulates IRF2-dependent H4 promoter activity.

タンパク質ファミリー:

Belongs to the IRF family.

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