Phospho-PKA R2 (Ser99) Antibody - #AF3724
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF3724, RRID:AB_2847038.
折りたたみ／展開
cAMP dependent protein kinase regulatory subunit alpha 2; cAMP dependent protein kinase regulatory subunit RII alpha; cAMP dependent protein kinase type II alpha regulatory chain; cAMP dependent protein kinase type II alpha regulatory subunit; cAMP-dependent protein kinase type II-alpha regulatory subunit; KAP2; KAP2_HUMAN; MGC3606; PKR 2; PKR2; PRKA R2; PRKAR 2; PRKAR2; PRKAR2A; Protein kinase A RII alpha subunit; Protein kinase cAMP dependent regulatory type II alpha;
免疫原
A synthesized peptide derived from human PKA R2 around the phosphorylation site of Ser99.
Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
- P13861 KAP2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSHIQIPPGLTELLQGYTVEVLRQQPPDLVEFAVEYFTRLREARAPASVLPAATPRQSLGHPPPEPGPDRVADAKGDSESEEDEDLEVPVPSRFNRRVSVCAETYNPDEEEEDTDPRVIHPKTDEQRCRLQEACKDILLFKNLDQEQLSQVLDAMFERIVKADEHVIDQGDDGDNFYVIERGTYDILVTKDNQTRSVGQYDNRGSFGELALMYNTPRAATIVATSEGSLWGLDRVTFRRIIVKNNAKKRKMFESFIESVPLLKSLEVSERMKIVDVIGEKIYKDGERIITQGEKADSFYIIESGEVSILIRSRTKSNKDGGNQEVEIARCHKGQYFGELALVTNKPRAASAYAVGDVKCLVMDVQAFERLLGPCMDIMKRNISHYEEQLVKMFGSSVDLGNLGQ
PTMs - P13861 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S48 | Phosphorylation | Uniprot | |
| T54 | Phosphorylation | Uniprot | |
| S58 | Phosphorylation | Uniprot | |
| S78 | Phosphorylation | Uniprot | |
| S80 | Phosphorylation | Uniprot | |
| S92 | Phosphorylation | Uniprot | |
| S99 | Phosphorylation | Uniprot | |
| C101 | S-Nitrosylation | Uniprot | |
| T104 | Phosphorylation | Uniprot | |
| Y105 | Phosphorylation | Uniprot | |
| T114 | Phosphorylation | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| K135 | Ubiquitination | Uniprot | |
| K161 | Ubiquitination | Uniprot | |
| Y177 | Phosphorylation | Uniprot | |
| K190 | Ubiquitination | Uniprot | |
| S196 | Phosphorylation | Uniprot | |
| Y200 | Phosphorylation | Uniprot | |
| S254 | Phosphorylation | Uniprot | |
| K263 | Ubiquitination | Uniprot | |
| S268 | Phosphorylation | Uniprot | |
| K272 | Ubiquitination | Uniprot | |
| K280 | Ubiquitination | Uniprot | |
| K315 | Methylation | Uniprot | |
| K318 | Acetylation | Uniprot | |
| K318 | Ubiquitination | Uniprot | |
| K332 | Ubiquitination | Uniprot | |
| T343 | Phosphorylation | Uniprot | |
| K345 | Ubiquitination | Uniprot | |
| S350 | Phosphorylation | Uniprot | |
| Y352 | Phosphorylation | Uniprot | |
| S383 | Phosphorylation | Uniprot | |
| Y385 | Phosphorylation | Uniprot | |
| S395 | Phosphorylation | Uniprot | |
| S396 | Phosphorylation | Uniprot |
研究背景
Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
Phosphorylated by the activated catalytic chain.
Cytoplasm. Cell membrane.
Note: Colocalizes with PJA2 in the cytoplasm and the cell membrane.
Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. Interacts with AKAP4 and CBFA2T3. Interacts with the phosphorylated form of PJA2. Interacts with MYRIP. This interaction may link PKA to components of the exocytosis machinery, thus facilitating exocytosis, including insulin release (By similarity). Forms a complex composed of PRKAR2A, GSK3B and GSKIP through GSKIP interaction; facilitates PKA-induced phosphorylation and regulates GSK3B activity. Interacts with ADCY8; inhibits adenylate cyclase activity through PKA phosphorylation.
Belongs to the cAMP-dependent kinase regulatory chain family.
研究領域
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
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