eIF4E Antibody - #AF6110

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製品: eIF4E Antibody
カタログ: AF6110
タンパク質の説明: Rabbit polyclonal antibody to eIF4E
アプリケーション: WB IHC IF/ICC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 25kDa; 25kD(Calculated).
ユニプロット: P06730
RRID: AB_2834997

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 100ul $280 在庫あり
 200ul $350 在庫あり

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関連ダウンロード
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
eIF4E Antibody detects endogenous levels of total eIF4E.
RRID:
AB_2834997
引用形式: Affinity Biosciences Cat# AF6110, RRID:AB_2834997.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

AUTS19; CBP; eIF 4E; eIF 4F 25 kDa subunit; EIF 4F; eIF-4E; eIF-4F 25 kDa subunit; eIF4E; EIF4E1; EIF4EL1; EIF4F; Eukaryotic translation initiation factor 4 E; Eukaryotic translation initiation factor 4E; Eukaryotic translation initiation factor 4E like 1; IF4E_HUMAN; Messanger RNA Cap Binding Protein eIF 4E; MGC111573; mRNA cap binding protein; mRNA cap-binding protein;

免疫原

免疫原:

A synthesized peptide derived from human eIF4E, corresponding to a region within C-terminal amino acids.

Uniprot:

P06730(IF4E_HUMAN) >>Visit HPA database.

遺伝子(ID):
EIF4E(1977)
タンパク質の説明:
eIF4E a protein of the eukaryotic initiation factor 4E family. Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
タンパク質配列:
MATVEPETTPTPNPPTTEEEKTESNQEVANPEHYIKHPLQNRWALWFFKNDKSKTWQANLRLISKFDTVEDFWALYNHIQLSSNLMPGCDYSLFKDGIEPMWEDEKNKRGGRWLITLNKQQRRSDLDRFWLETLLCLIGESFDDYSDDVCGAVVNVRAKGDKIAIWTTECENREAVTHIGRVYKERLGLPPKIVIGYQSHADTATKSGSTTKNRFVV

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.

PTMs:

Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.

細胞の位置付け:

Cytoplasm>P-body. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the eukaryotic initiation factor 4E family.

研究領域

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Genetic Information Processing > Translation > RNA transport.

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

参考文献

1). A novel lncRNA SNHG29 regulates EP300- related histone acetylation modification and inhibits FLT3-ITD AML development. Leukemia, 2023 (PubMed: 37157016) [IF=12.8]
2). Effects of Bisphenol A on reproductive toxicity and gut microbiota dysbiosis in male rats. Ecotoxicology and Environmental Safety, 2022 (PubMed: 35567931) [IF=6.2]

Application: WB    Species: Rat    Sample: testes tissue

Fig. 3. Effects of BPA exposure on PI3K/AKT/mTOR pathway in testis (A-C) The mRNA expression levels of Pi3k, Akt1, and mtor in testis. (D-K) The protein expression levels of PI3K, mTOR, p-AKT, p-AKT/AKT, Raptor, 4EBP1, and eIF4E in testis. Different letters indicate significant differences between groups (P 

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