Phospho-TIF1 alpha (Ser649) Antibody - #AF3774
| 製品: | Phospho-TIF1 alpha (Ser649) Antibody |
| カタログ: | AF3774 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-TIF1 alpha (Ser649) |
| アプリケーション: | WB |
| 反応性: | Human |
| 分子量: | 74kD(Calculated). |
| ユニプロット: | Q9NYV6 |
| RRID: | AB_2847088 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF3774, RRID:AB_2847088.
折りたたみ／展開
AL023001; DKFZp566E104; E130302O19Rik; MGC104238; R75565; RGD1305001; RNA polymerase I-specific transcription initiation factor RRN3; Rrn3; RRN3 RNA polymerase I transcription factor homolog (S. cerevisiae); RRN3_HUMAN; TIF-IA; TIF1A; TIFIA; Transcription Initiation Factor 1A; Transcription initiation factor IA; Transcription initiation factor TIF IA;
免疫原
A synthesized peptide derived from human TIF1 alpha around the phosphorylation site of Ser649.
- Q9NYV6 RRN3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAPLLHTRLPGDAAASSSAVKKLGASRTGISNMRALENDFFNSPPRKTVRFGGTVTEVLLKYKKGETNDFELLKNQLLDPDIKDDQIINWLLEFRSSIMYLTKDFEQLISIILRLPWLNRSQTVVEEYLAFLGNLVSAQTVFLRPCLSMIASHFVPPRVIIKEGDVDVSDSDDEDDNLPANFDTCHRALQIIARYVPSTPWFLMPILVEKFPFVRKSERTLECYVHNLLRISVYFPTLRHEILELIIEKLLKLDVNASRQGIEDAEETATQTCGGTDSTEGLFNMDEDEETEHETKAGPERLDQMVHPVAERLDILMSLVLSYMKDVCYVDGKVDNGKTKDLYRDLINIFDKLLLPTHASCHVQFFMFYLCSFKLGFAEAFLEHLWKKLQDPSNPAIIRQAAGNYIGSFLARAKFIPLITVKSCLDLLVNWLHIYLNNQDSGTKAFCDVALHGPFYSACQAVFYTFVFRHKQLLSGNLKEGLQYLQSLNFERIVMSQLNPLKICLPSVVNFFAAITNKYQLVFCYTIIERNNRQMLPVIRSTAGGDSVQICTNPLDTFFPFDPCVLKRSKKFIDPIYQVWEDMSAEELQEFKKPMKKDIVEDEDDDFLKGEVPQNDTVIGITPSSFDTHFRSPSSSVGSPPVLYMQPSPL
PTMs - Q9NYV6 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R9 | Methylation | Uniprot | |
| S19 | Phosphorylation | Uniprot | |
| K22 | Ubiquitination | Uniprot | |
| S44 | Phosphorylation | P24941 (CDK2) | Uniprot |
| K62 | Ubiquitination | Uniprot | |
| K65 | Ubiquitination | Uniprot | |
| K75 | Ubiquitination | Uniprot | |
| S170 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S172 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S199 | Phosphorylation | Uniprot | |
| T200 | Phosphorylation | P45984 (MAPK9) | Uniprot |
| S233 | Phosphorylation | Uniprot | |
| K253 | Ubiquitination | Uniprot | |
| T271 | Phosphorylation | Uniprot | |
| S319 | Phosphorylation | Uniprot | |
| K334 | Ubiquitination | Uniprot | |
| K415 | Ubiquitination | Uniprot | |
| K480 | Ubiquitination | Uniprot | |
| K568 | Ubiquitination | Uniprot | |
| K593 | Ubiquitination | Uniprot | |
| K598 | Ubiquitination | Uniprot | |
| K610 | Ubiquitination | Uniprot | |
| T623 | Phosphorylation | Uniprot | |
| S633 | Phosphorylation | P27361 (MAPK3) | Uniprot |
| S635 | Phosphorylation | Q13131 (PRKAA1) | Uniprot |
| S636 | Phosphorylation | Uniprot | |
| S640 | Phosphorylation | Uniprot | |
| S649 | Phosphorylation | P51812 (RPS6KA3) , P27361 (MAPK3) | Uniprot |
研究背景
Required for efficient transcription initiation by RNA polymerase I. Required for the formation of the competent preinitiation complex (PIC). Dissociates from pol I as a consequence of transcription. In vitro, cannot activate transcription in a subsequent transcription reaction (By similarity).
Phosphorylation is required for participation in rDNA transcription (By similarity). Phosphorylated at Thr-200 by MAPK9/JNK2, which abrogates initiation complex formation.
Nucleus>Nucleolus.
Interacts with TWISTNB, EIF3L, TAF1B and TAF1C.
Belongs to the RRN3 family.
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