Phospho-HEC1 (Ser165) Antibody - #AF3780
| 製品: | Phospho-HEC1 (Ser165) Antibody |
| カタログ: | AF3780 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-HEC1 (Ser165) |
| アプリケーション: | ELISA(peptide) |
| 反応性: | Human, Mouse |
| 分子量: | 74kD(Calculated). |
| ユニプロット: | O14777 |
| RRID: | AB_2847094 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF3780, RRID:AB_2847094.
折りたたみ／展開
from Entrez Gene ;; HEC; Highly expressed in cancer; Highly expressed in cancer protein; Highly expressed in cancer rich in leucine heptad repeats; HsHec1; hsNDC80; Kinetochore associated 2; Kinetochore associated protein 2; Kinetochore protein Hec1; Kinetochore protein NDC80 homolog; Kinetochore-associated protein 2; KNTC2; ndc80; NDC80 homolog kinetochore complex component; NDC80 kinetochore complex component homolog; NDC80, S. cerevisiae, homolog of; NDC80_HUMAN; Retinoblastoma associated protein HEC; Retinoblastoma-associated protein HEC; TID3;
免疫原
A synthesized peptide derived from human HEC1 around the phosphorylation site of Ser165.
- O14777 NDC80_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKRSSVSSGGAGRLSMQELRSQDVNKQGLYTPQTKEKPTFGKLSINKPTSERKVSLFGKRTSGHGSRNSQLGIFSSSEKIKDPRPLNDKAFIQQCIRQLCEFLTENGYAHNVSMKSLQAPSVKDFLKIFTFLYGFLCPSYELPDTKFEEEVPRIFKDLGYPFALSKSSMYTVGAPHTWPHIVAALVWLIDCIKIHTAMKESSPLFDDGQPWGEETEDGIMHNKLFLDYTIKCYESFMSGADSFDEMNAELQSKLKDLFNVDAFKLESLEAKNRALNEQIARLEQEREKEPNRLESLRKLKASLQGDVQKYQAYMSNLESHSAILDQKLNGLNEEIARVELECETIKQENTRLQNIIDNQKYSVADIERINHERNELQQTINKLTKDLEAEQQKLWNEELKYARGKEAIETQLAEYHKLARKLKLIPKGAENSKGYDFEIKFNPEAGANCLVKYRAQVYVPLKELLNETEEEINKALNKKMGLEDTLEQLNAMITESKRSVRTLKEEVQKLDDLYQQKIKEAEEEDEKCASELESLEKHKHLLESTVNQGLSEAMNELDAVQREYQLVVQTTTEERRKVGNNLQRLLEMVATHVGSVEKHLEEQIAKVDREYEECMSEDLSENIKEIRDKYEKKATLIKSSEE
PTMs - O14777 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S5 | Phosphorylation | Q96GD4 (AURKB) | Uniprot |
| S15 | Phosphorylation | Q96GD4 (AURKB) | Uniprot |
| R20 | Methylation | Uniprot | |
| K26 | Ubiquitination | Uniprot | |
| Y30 | Phosphorylation | Uniprot | |
| T31 | Phosphorylation | Uniprot | |
| T34 | Phosphorylation | Uniprot | |
| K37 | Ubiquitination | Uniprot | |
| T39 | Phosphorylation | Uniprot | |
| K42 | Ubiquitination | Uniprot | |
| S44 | Phosphorylation | Q96GD4 (AURKB) | Uniprot |
| K47 | Ubiquitination | Uniprot | |
| T49 | Phosphorylation | Q96GD4 (AURKB) | Uniprot |
| S50 | Phosphorylation | Uniprot | |
| K53 | Acetylation | Uniprot | |
| S55 | Phosphorylation | Q96GD4 (AURKB) , O14965 (AURKA) | Uniprot |
| K59 | Acetylation | Uniprot | |
| K59 | Methylation | Uniprot | |
| K59 | Ubiquitination | Uniprot | |
| T61 | Phosphorylation | Uniprot | |
| S62 | Phosphorylation | Uniprot | |
| S66 | Phosphorylation | Uniprot | |
| S69 | Phosphorylation | Q96GD4 (AURKB) | Uniprot |
| S76 | Phosphorylation | Uniprot | |
| S77 | Phosphorylation | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| K89 | Ubiquitination | Uniprot | |
| K123 | Ubiquitination | Uniprot | |
| R153 | Methylation | Uniprot | |
| K156 | Ubiquitination | Uniprot | |
| S165 | Phosphorylation | P51955 (NEK2) , Q96PY6 (NEK1) | Uniprot |
| S202 | Phosphorylation | Uniprot | |
| K253 | Ubiquitination | Uniprot | |
| K255 | Ubiquitination | Uniprot | |
| K264 | Ubiquitination | Uniprot | |
| K271 | Ubiquitination | Uniprot | |
| K300 | Ubiquitination | Uniprot | |
| K309 | Ubiquitination | Uniprot | |
| K327 | Ubiquitination | Uniprot | |
| K346 | Ubiquitination | Uniprot | |
| K360 | Ubiquitination | Uniprot | |
| Y361 | Phosphorylation | Uniprot | |
| K382 | Ubiquitination | Uniprot | |
| K393 | Ubiquitination | Uniprot | |
| K400 | Ubiquitination | Uniprot | |
| K405 | Ubiquitination | Uniprot | |
| K417 | Ubiquitination | Uniprot | |
| K433 | Ubiquitination | Uniprot | |
| K452 | Ubiquitination | Uniprot | |
| Y458 | Phosphorylation | Uniprot | |
| K462 | Ubiquitination | Uniprot | |
| K474 | Ubiquitination | Uniprot | |
| T502 | Phosphorylation | Uniprot | |
| K504 | Ubiquitination | Uniprot | |
| K509 | Ubiquitination | Uniprot | |
| K517 | Ubiquitination | Uniprot | |
| K527 | Acetylation | Uniprot | |
| S530 | Phosphorylation | Uniprot | |
| T591 | Phosphorylation | Uniprot | |
| S595 | Phosphorylation | Uniprot | |
| K606 | Ubiquitination | Uniprot | |
| S616 | Phosphorylation | Uniprot |
研究背景
Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules. Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules.
Phosphorylation begins in S phase of the cell cycle and peaks in mitosis. Phosphorylated by NEK2. May also be phosphorylated by AURKA and AURKB.
Nucleus. Chromosome>Centromere>Kinetochore.
Note: Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore.
Component of the NDC80 complex, which consists of NDC80/HEC1, CDCA1, SPBC24 and SPBC25. The NDC80 complex is formed by two subcomplexes composed of NDC80/HEC1-CDCA1 and SPBC24-SPBC25. Each subcomplex is formed by parallel interactions through the coiled-coil domains of individual subunits. Formation of a tetrameric complex is mediated by interactions between the C-terminal regions of both subunits of the NDC80/HEC1-CDCA1 subcomplex and the N-terminal regions of both subunits of the SPBC24-SPBC25 complex. The tetrameric NDC80 complex has an elongated rod-like structure with globular domains at either end. Interacts with isoform 1 of NEK2 and ZWINT specifically during mitosis. Interacts with CENPH and MIS12. May interact with AURKB, PSMC2, PSMC5 and SMC1A. May interact with RB1 during G2 phase and mitosis. Interacts with CKAP5. Interacts with CDT1; leading to kinetochore localization of CDT1.
Belongs to the NDC80/HEC1 family.
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