Phospho-HER3/ErbB3 (Tyr1289) Antibody - #AF3802

製品: Phospho-HER3/ErbB3 (Tyr1289) Antibody
カタログ: AF3802
タンパク質の説明: Rabbit polyclonal antibody to Phospho-HER3/ErbB3 (Tyr1289)
アプリケーション: ELISA(peptide)
反応性: Human, Mouse, Rat
分子量: 148kD(Calculated).
ユニプロット: P21860
RRID: AB_2847116

類似製品を見る>>

   サイズ 価格 在庫状況
 100ul $350 在庫あり
 200ul $450 在庫あり

リードタイム: 当日配達

For pricing and ordering contact:
お問い合わせ先
関連ダウンロード
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
Phospho-HER3/ErbB3 (Tyr1289) Antibody detects endogenous levels of HER3/ErbB3 only when phosphorylated at Tyr1289.
RRID:
AB_2847116
引用形式: Affinity Biosciences Cat# AF3802, RRID:AB_2847116.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

c erbB 3; c erbB3; Erb b2 receptor tyrosine kinase 3; ErbB 3; ERBB3; ERBB3 protein; erbB3 S; ERBB3_HUMAN; Glial growth factor receptor; HER 3; HER3; Human epidermal growth factor receptor 3; LCCS2; MDA BF 1; MGC88033; p180 ErbB3; p45 sErbB3; p85 sErbB3; proto-oncogene-like protein c ErbB 3; proto-oncogene-like protein c ErbB3; Proto-oncogene-like protein c-ErbB-3; Receptor tyrosine protein kinase erbB 3; Receptor tyrosine protein kinase erbB3; Receptor tyrosine-protein kinase erbB-3; Tyrosine kinase type cell surface receptor HER3; Tyrosine kinase-type cell surface receptor HER3; v erb b2 avian erythroblastic leukemia viral oncogene homolog 3; v erb b2 erythroblastic leukemia viral oncogene homolog 3 (avian); v erb b2 erythroblastic leukemia viral oncogene homolog 3;

免疫原

免疫原:

A synthesized peptide derived from human HER3/ErbB3 around the phosphorylation site of Tyr1289.

Uniprot:

P21860(ERBB3_HUMAN) >>Visit HPA database.

遺伝子(ID):
ERBB3(2065)
発現特異性:
P21860 ERBB3_HUMAN:

Epithelial tissues and brain.

タンパク質配列:
MRANDALQVLGLLFSLARGSEVGNSQAVCPGTLNGLSVTGDAENQYQTLYKLYERCEVVMGNLEIVLTGHNADLSFLQWIREVTGYVLVAMNEFSTLPLPNLRVVRGTQVYDGKFAIFVMLNYNTNSSHALRQLRLTQLTEILSGGVYIEKNDKLCHMDTIDWRDIVRDRDAEIVVKDNGRSCPPCHEVCKGRCWGPGSEDCQTLTKTICAPQCNGHCFGPNPNQCCHDECAGGCSGPQDTDCFACRHFNDSGACVPRCPQPLVYNKLTFQLEPNPHTKYQYGGVCVASCPHNFVVDQTSCVRACPPDKMEVDKNGLKMCEPCGGLCPKACEGTGSGSRFQTVDSSNIDGFVNCTKILGNLDFLITGLNGDPWHKIPALDPEKLNVFRTVREITGYLNIQSWPPHMHNFSVFSNLTTIGGRSLYNRGFSLLIMKNLNVTSLGFRSLKEISAGRIYISANRQLCYHHSLNWTKVLRGPTEERLDIKHNRPRRDCVAEGKVCDPLCSSGGCWGPGPGQCLSCRNYSRGGVCVTHCNFLNGEPREFAHEAECFSCHPECQPMEGTATCNGSGSDTCAQCAHFRDGPHCVSSCPHGVLGAKGPIYKYPDVQNECRPCHENCTQGCKGPELQDCLGQTLVLIGKTHLTMALTVIAGLVVIFMMLGGTFLYWRGRRIQNKRAMRRYLERGESIEPLDPSEKANKVLARIFKETELRKLKVLGSGVFGTVHKGVWIPEGESIKIPVCIKVIEDKSGRQSFQAVTDHMLAIGSLDHAHIVRLLGLCPGSSLQLVTQYLPLGSLLDHVRQHRGALGPQLLLNWGVQIAKGMYYLEEHGMVHRNLAARNVLLKSPSQVQVADFGVADLLPPDDKQLLYSEAKTPIKWMALESIHFGKYTHQSDVWSYGVTVWELMTFGAEPYAGLRLAEVPDLLEKGERLAQPQICTIDVYMVMVKCWMIDENIRPTFKELANEFTRMARDPPRYLVIKRESGPGIAPGPEPHGLTNKKLEEVELEPELDLDLDLEAEEDNLATTTLGSALSLPVGTLNRPRGSQSLLSPSSGYMPMNQGNLGESCQESAVSGSSERCPRPVSLHPMPRGCLASESSEGHVTGSEAELQEKVSMCRSRSRSRSPRPRGDSAYHSQRHSLLTPVTPLSPPGLEEEDVNGYVMPDTHLKGTPSSREGTLSSVGLSSVLGTEEEDEDEEYEYMNRRRRHSPPHPPRPSSLEELGYEYMDVGSDLSASLGSTQSCPLHPVPIMPTAGTTPDEDYEYMNRQRDGGGPGGDYAAMGACPASEQGYEEMRAFQGPGHQAPHVHYARLKTLRSLEATDSAFDNPDYWHSRLFPKANAQRT

PTMs - P21860 基板として

Site PTM Type Enzyme
S15 Phosphorylation
N250 N-Glycosylation
N353 N-Glycosylation
N408 N-Glycosylation
N414 N-Glycosylation
N437 N-Glycosylation
N469 N-Glycosylation
N522 N-Glycosylation
N566 N-Glycosylation
T640 Phosphorylation
Y680 Phosphorylation
S686 Phosphorylation
S693 Phosphorylation
S717 Phosphorylation
K736 Methylation
S844 Phosphorylation
S846 Phosphorylation
Y868 Phosphorylation
S869 Phosphorylation
S982 Phosphorylation
T996 Phosphorylation
Y1054 Phosphorylation
S1083 Phosphorylation
S1094 Phosphorylation
S1104 Phosphorylation
S1113 Phosphorylation
Y1132 Phosphorylation
S1147 Phosphorylation
Y1159 Phosphorylation
T1164 Phosphorylation
Y1197 Phosphorylation
Y1199 Phosphorylation
S1207 Phosphorylation
S1215 Phosphorylation
Y1222 Phosphorylation
Y1260 Phosphorylation
Y1262 Phosphorylation
Y1276 Phosphorylation P00533 (EGFR)
S1285 Phosphorylation
Y1289 Phosphorylation P00533 (EGFR)
Y1307 Phosphorylation Q01973 (ROR1)
T1312 Phosphorylation
S1315 Phosphorylation
T1319 Phosphorylation
Y1328 Phosphorylation
S1331 Phosphorylation

研究背景

機能:

Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins. Binds to neuregulin-1 (NRG1) and is activated by it; ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase. May also be activated by CSPG5. Involved in the regulation of myeloid cell differentiation.

PTMs:

Autophosphorylated. Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase.

細胞の位置付け:

Cell membrane>Single-pass type I membrane protein.

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Epithelial tissues and brain.

サブユニット構造:

Monomer and homodimer. Heterodimer with each of the other ERBB receptors (Potential). Interacts with CSPG5. Interacts with GRB7. Interacts with MUC1. Interacts with MYOC (By similarity). Interacts with isoform 2 of PA2G4. Found in a ternary complex with NRG1 and ITGAV:ITGB3 or ITGA6:ITGB4.

タンパク質ファミリー:

The cytoplasmic part of the receptor may interact with the SH2 or SH3 domains of many signal-transducing proteins.

Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.

研究領域

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.