ADRB2 Antibody - #AF6117

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製品: ADRB2 Antibody
カタログ: AF6117
タンパク質の説明: Rabbit polyclonal antibody to ADRB2
アプリケーション: WB IHC IF/ICC
Cited expt.: WB, IHC, IF/ICC
反応性: Human, Mouse
予測: Bovine, Rabbit, Dog
分子量: 47kDa; 46kD(Calculated).
ユニプロット: P07550
RRID: AB_2835001

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説明書
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WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Bovine(100%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
ADRB2 Antibody detects endogenous levels of total ADRB2.
RRID:
AB_2835001
引用形式: Affinity Biosciences Cat# AF6117, RRID:AB_2835001.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

ADRB2; ADRB2_HUMAN; ADRB2R; ADRBR; Adrenergic beta 2 receptor surface; Adrenoceptor beta 2 surface; B2AR; BAR; beta 2 adrenoceptor; Beta 2 adrenoreceptor; Beta-2 adrenergic receptor; Beta-2 adrenoceptor; Beta-2 adrenoreceptor; BETA2AR; Catecholamine receptor; OTTHUMP00000160386;

免疫原

免疫原:

A synthesized peptide derived from human ADRB2, corresponding to a region within C-terminal amino acids.

Uniprot:

P07550(ADRB2_HUMAN) >>Visit HPA database.

遺伝子(ID):
ADRB2(154)
タンパク質の説明:
This gene encodes beta-2-adrenergic receptor which is a member of the G protein-coupled receptor superfamily. This receptor is directly associated with one of its ultimate effectors, the class C L-type calcium channel Ca(V)1.2.
タンパク質配列:
MGQPGNGSAFLLAPNGSHAPDHDVTQERDEVWVVGMGIVMSLIVLAIVFGNVLVITAIAKFERLQTVTNYFITSLACADLVMGLAVVPFGAAHILMKMWTFGNFWCEFWTSIDVLCVTASIETLCVIAVDRYFAITSPFKYQSLLTKNKARVIILMVWIVSGLTSFLPIQMHWYRATHQEAINCYANETCCDFFTNQAYAIASSIVSFYVPLVIMVFVYSRVFQEAKRQLQKIDKSEGRFHVQNLSQVEQDGRTGHGLRRSSKFCLKEHKALKTLGIIMGTFTLCWLPFFIVNIVHVIQDNLIRKEVYILLNWIGYVNSGFNPLIYCRSPDFRIAFQELLCLRRSSLKAYGNGYSSNGNTGEQSGYHVEQEKENKLLCEDLPGTEDFVGHQGTVPSDNIDSQGRNCSTNDSLL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Dog
100
Rabbit
100
Pig
0
Horse
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine.

PTMs:

Palmitoylated; may reduce accessibility of Ser-345 and Ser-346 by anchoring Cys-341 to the plasma membrane. Agonist stimulation promotes depalmitoylation and further allows Ser-345 and Ser-346 phosphorylation.

Phosphorylated by PKA and BARK upon agonist stimulation, which mediates homologous desensitization of the receptor. PKA-mediated phosphorylation seems to facilitate phosphorylation by BARK.

Phosphorylation of Tyr-141 is induced by insulin and leads to supersensitization of the receptor.

Polyubiquitinated. Agonist-induced ubiquitination leads to sort internalized receptors to the lysosomes for degradation. Deubiquitination by USP20 and USP33, leads to ADRB2 recycling and resensitization after prolonged agonist stimulation. USP20 and USP33 are constitutively associated and are dissociated immediately after agonist stimulation. Ubiquitination by the VHL-E3 ligase complex is oxygen-dependent.

Hydroxylation by EGLN3 occurs only under normoxia and increases the interaction with VHL and the subsequent ubiquitination and degradation of ADRB2.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein. Early endosome.
Note: Colocalizes with VHL at the cell membrane (PubMed:19584355). Activated receptors are internalized into endosomes prior to their degradation in lysosomes (PubMed:20559325).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB2 sub-subfamily.

研究領域

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

· Organismal Systems > Endocrine system > Renin secretion.

· Organismal Systems > Digestive system > Salivary secretion.

参考文献

1). Accurate Transcription Factor Activity Inference to Decipher Cell Identity from Single-Cell Transcriptomic Data with MetaTF. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025 (PubMed: 40397381) [IF=15.1]

Application: IF/ICC    Species: human    Sample:

Figure 5 metaTF reveals TF activity heterogeneity in human breast cancer T cells. a) UMAP plots visualizing cluster assignments of human breast cancer T cells based on TF activity profile (left) and highly variable gene expression profile (right). Cells are color-coded to represent different human breast cancer T-cell populations, including TEM (effector/memory T cells), TEX (experienced T cells), TN (naive T cells), TREG (regulatory T cells), natural killer (NK) cells), and Proliferating (proliferating T cells). b) UMAP plot of CD8+ TEM C1 and C2 marker genes expression. c) Heatmap plot showing the expression of the top 50 differentially expressed genes between CD8+ TEM C1 and C2 cell populations. d) GO biological process enrichment results for the differentially expressed genes between CD8+ TEM C1 (upper panel) and C2 (lower panel) cell populations respectively. e) Dot plot showing the significant expression of β2-adrenergic receptor (ADRB2) and adrenoceptor alpha 2B (ADRA2B) primarily in the CD8+ TEM C1 cell population. f) Dot plot indicating that BCL6 was specifically activated in the CD8+ TEM C1 cell population. g) Spatial co-mapping of CD8 and ADRB2 gene expression by spatial transcriptomics of a sample from a breast cancer patient recorded in a public dataset (GSE195665, patient35, right panel). The left and middle panels depict expression levels in fresh frozen sections. h) Expression of CD8 and ADRB2 in breast cancer tissue, and the colocalization (Pearson's coefficient) between CD8 and ADRB2 is shown, n = 12. i) Upregulated targets of BCL6 in the epinephrine treatment group compared to the control group from in vitro cytological experiments. j) In vitro cytological experiments confirmed that candidate TFs and their targets are highly expressed in the epinephrine-treated group through GSEA prerank analysis.
2). Mammalian Eps15 homology domain 1 potentiates angiogenesis of non-small cell lung cancer by regulating β2AR signaling. Journal of Experimental & Clinical Cancer Research, 2019 (PubMed: 31023336) [IF=11.3]

Application: WB    Species: mouse    Sample: xenograft tumo

Fig. 6| Targeting of VEGFA represses EHD1-induced tumor growth and angiogenesis in vivo. a-b The expression of EHD1, β2AR and VEGFA in freshly frozen xenograft tumor tissue was analyzed by Western blot.

Application: IHC    Species: human    Sample: HCC

Figure S9. |Correlation of EHD1 with β2AR, VEGFA and CD31 expression and angiogenesis in HCC tissues. a Representative IHC images of NSCLC samples with low and high expression of the indicated proteins. Scale bar, 50 μm and 100 μm.
3). Quercetin inhibits chronic stress-mediated progression of triple-negative breast cancer by blocking β2-AR/ERK1/2 pathway. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2024 (PubMed: 38901200) [IF=6.9]
4). Baicalin reduces chronic stress-induced breast cancer metastasis via directly targeting β2-adrenergic receptor. Journal of pharmaceutical analysis, 2024 (PubMed: 39139999) [IF=6.1]
5). ADRB2 serves as a novel biomarker and attenuates alcoholic hepatitis via the SIRT1/PGC-1α/PPARα pathway: integration of WGCNA, machine learning and experimental validation. Frontiers in pharmacology, 2024 (PubMed: 39640486) [IF=5.6]

Application: IHC    Species: human    Sample:

Figure 4. Identification and verification of the diagnostic biomarkers of alcoholic hepatitis. (A) From the chosen modules, diagnostic markers were screened using the Least Absolute Shrinkage and Selection Operator (LASSO) logistic regression approach. (B) Diagnostic nomogram. (C) Receiver operating characteristic curves to assess the diagnostic ability. (D) Box plots for the differential expression analysis in the metadata. (E) Box plots for the ADRB2 differential expression analysis in the testing data sets. (F) Immunohistochemical analysis of ADRB2 expression in normal groups and patients with alcoholic hepatitis.

Application: WB    Species: Mouse    Sample: liver tissues

Figure 8. Low expression of ADRB2, SIRT1, PGC-1α, and PPARα in ethanol-induced alcoholic hepatitis. (A) Western blot analysis of ADRB2 in NIAAA mouse liver tissues. (B) Statistical results of NIAAA mouse liver western blot analysis. (C) Immunofluorescence of ADRB2 and SIRT1 in NIAAA mouse liver tissue. (D) Western blot analysis of ADRB2, PPARα, PGC-1α, and SIRT1 in ALD mouse liver tissues. (E) Statistical results of ALD mouse liver western blot analysis. (F) Immunofluorescence of ADRB2 and SIRT1 in ALD mouse liver tissue. * Represents p < 0.05 and ** represents p < 0.01, compared with the control group.

Application: IF/ICC    Species: Mouse    Sample: liver tissues

Figure 8. Low expression of ADRB2, SIRT1, PGC-1α, and PPARα in ethanol-induced alcoholic hepatitis. (A) Western blot analysis of ADRB2 in NIAAA mouse liver tissues. (B) Statistical results of NIAAA mouse liver western blot analysis. (C) Immunofluorescence of ADRB2 and SIRT1 in NIAAA mouse liver tissue. (D) Western blot analysis of ADRB2, PPARα, PGC-1α, and SIRT1 in ALD mouse liver tissues. (E) Statistical results of ALD mouse liver western blot analysis. (F) Immunofluorescence of ADRB2 and SIRT1 in ALD mouse liver tissue. * Represents p < 0.05 and ** represents p < 0.01, compared with the control group.
6). -bambuterol and its enantiomers: Potential improvement of (R)-bambuterol in mice with colitis. International Immunopharmacology, 2022 (PubMed: 34974400) [IF=4.8]

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