製品: Phospho-RSK1/2/3/4 (Ser221/Ser227/Ser218/Ser232) Antibody
カタログ: AF3810
タンパク質の説明: Rabbit polyclonal antibody to Phospho-RSK1/2/3/4 (Ser221/Ser227/Ser218/Ser232)
アプリケーション: WB IHC
反応性: Human, Mouse, Rat, Monkey
分子量: 83kD,84kD(Calculated).
ユニプロット: Q15418 | P51812 | Q15349 | Q9UK32
RRID: AB_2847124

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製品説明

ソース:
Rabbit
アプリケーション:
IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat,Monkey
クローナリティ:
Polyclonal
特異性:
Phospho-RSK1/2/3/4 (Ser221/Ser227/Ser218/Ser232) Antibody detects endogenous levels of RSK1/2/3/4 only when phosphorylated at Ser221/227/s218/232.
RRID:
AB_2847124
引用形式: Affinity Biosciences Cat# AF3810, RRID:AB_2847124.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

90 kDa ribosomal protein S6 kinase 3; CLS; HU 3; HU2; HU3; Insulin stimulated protein kinase 1; Insulin-stimulated protein kinase 1; ISPK-1; ISPK1; KS6A3_HUMAN; MAP kinase activated protein kinase 1b; MAP kinase-activated protein kinase 1b; MAPK activated protein kinase 1b; MAPK-activated protein kinase 1b; MAPKAP kinase 1b; MAPKAPK 1b; MAPKAPK-1b; MAPKAPK1B; Mental retardation, X linked 19; MRX19; OTTHUMP00000023036; p90 RSK2; p90 RSK3; p90-RSK 3; p90RSK3; pp90RSK2; Ribosomal protein S6 kinase 90kDa polypeptide 3; Ribosomal protein S6 kinase alpha 3; Ribosomal protein S6 kinase alpha-3; Ribosomal protein s6 kinase ii alpha 2; Ribosomal S6 kinase 2; Rps6ka3; RSK; RSK-2; RSK2; S6 kinase 2; S6K alpha3; S6K-alpha-3; 90 kDa ribosomal protein S6 kinase 2; HU 2; KS6A2_HUMAN; MAP kinase activated protein kinase 1c; MAP kinase-activated protein kinase 1c; MAPK-activated protein kinase 1c; MAPKAP kinase 1c; MAPKAPK-1c; MAPKAPK1C; Mitogen.activated protein kinase-activated protein kinase 1C; p90 RSK3; p90-RSK 2; p90RSK

免疫原

免疫原:

A synthesized peptide derived from human RSK1/2/3/4 around the phosphorylation site of Ser221/227/s218/232.

Uniprot:
遺伝子(ID):
発現特異性:
P51812 KS6A3_HUMAN:

Expressed in many tissues, highest levels in skeletal muscle.

Q15349 KS6A2_HUMAN:

Widely expressed with higher expression in lung, skeletal muscle, brain, uterus, ovary, thyroid and prostate.

タンパク質配列:
MPLAQLKEPWPLMELVPLDPENGQTSGEEAGLQPSKDEGVLKEISITHHVKAGSEKADPSHFELLKVLGQGSFGKVFLVRKVTRPDSGHLYAMKVLKKATLKVRDRVRTKMERDILADVNHPFVVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALGLDHLHSLGIIYRDLKPENILLDEEGHIKLTDFGLSKEAIDHEKKAYSFCGTVEYMAPEVVNRQGHSHSADWWSYGVLMFEMLTGSLPFQGKDRKETMTLILKAKLGMPQFLSTEAQSLLRALFKRNPANRLGSGPDGAEEIKRHVFYSTIDWNKLYRREIKPPFKPAVAQPDDTFYFDTEFTSRTPKDSPGIPPSAGAHQLFRGFSFVATGLMEDDGKPRAPQAPLHSVVQQLHGKNLVFSDGYVVKETIGVGSYSECKRCVHKATNMEYAVKVIDKSKRDPSEEIEILLRYGQHPNIITLKDVYDDGKHVYLVTELMRGGELLDKILRQKFFSEREASFVLHTIGKTVEYLHSQGVVHRDLKPSNILYVDESGNPECLRICDFGFAKQLRAENGLLMTPCYTANFVAPEVLKRQGYDEGCDIWSLGILLYTMLAGYTPFANGPSDTPEEILTRIGSGKFTLSGGNWNTVSETAKDLVSKMLHVDPHQRLTAKQVLQHPWVTQKDKLPQSQLSHQDLQLVKGAMAATYSALNSSKPTPQLKPIESSILAQRRVRKLPSTTL

MPLAQLADPWQKMAVESPSDSAENGQQIMDEPMGEEEINPQTEEVSIKEIAITHHVKEGHEKADPSQFELLKVLGQGSFGKVFLVKKISGSDARQLYAMKVLKKATLKVRDRVRTKMERDILVEVNHPFIVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALALDHLHSLGIIYRDLKPENILLDEEGHIKLTDFGLSKESIDHEKKAYSFCGTVEYMAPEVVNRRGHTQSADWWSFGVLMFEMLTGTLPFQGKDRKETMTMILKAKLGMPQFLSPEAQSLLRMLFKRNPANRLGAGPDGVEEIKRHSFFSTIDWNKLYRREIHPPFKPATGRPEDTFYFDPEFTAKTPKDSPGIPPSANAHQLFRGFSFVAITSDDESQAMQTVGVHSIVQQLHRNSIQFTDGYEVKEDIGVGSYSVCKRCIHKATNMEFAVKIIDKSKRDPTEEIEILLRYGQHPNIITLKDVYDDGKYVYVVTELMKGGELLDKILRQKFFSEREASAVLFTITKTVEYLHAQGVVHRDLKPSNILYVDESGNPESIRICDFGFAKQLRAENGLLMTPCYTANFVAPEVLKRQGYDAACDIWSLGVLLYTMLTGYTPFANGPDDTPEEILARIGSGKFSLSGGYWNSVSDTAKDLVSKMLHVDPHQRLTAALVLRHPWIVHWDQLPQYQLNRQDAPHLVKGAMAATYSALNRNQSPVLEPVGRSTLAQRRGIKKITSTAL

MDLSMKKFAVRRFFSVYLRRKSRSKSSSLSRLEEEGVVKEIDISHHVKEGFEKADPSQFELLKVLGQGSYGKVFLVRKVKGSDAGQLYAMKVLKKATLKVRDRVRSKMERDILAEVNHPFIVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALALDHLHSLGIIYRDLKPENILLDEEGHIKITDFGLSKEAIDHDKRAYSFCGTIEYMAPEVVNRRGHTQSADWWSFGVLMFEMLTGSLPFQGKDRKETMALILKAKLGMPQFLSGEAQSLLRALFKRNPCNRLGAGIDGVEEIKRHPFFVTIDWNTLYRKEIKPPFKPAVGRPEDTFHFDPEFTARTPTDSPGVPPSANAHHLFRGFSFVASSLIQEPSQQDLHKVPVHPIVQQLHGNNIHFTDGYEIKEDIGVGSYSVCKRCVHKATDTEYAVKIIDKSKRDPSEEIEILLRYGQHPNIITLKDVYDDGKFVYLVMELMRGGELLDRILRQRYFSEREASDVLCTITKTMDYLHSQGVVHRDLKPSNILYRDESGSPESIRVCDFGFAKQLRAGNGLLMTPCYTANFVAPEVLKRQGYDAACDIWSLGILLYTMLAGFTPFANGPDDTPEEILARIGSGKYALSGGNWDSISDAAKDVVSKMLHVDPHQRLTAMQVLKHPWVVNREYLSPNQLSRQDVHLVKGAMAATYFALNRTPQAPRLEPVLSSNLAQRRGMKRLTSTRL

MLPFAPQDEPWDREMEVFSGGGASSGEVNGLKMVDEPMEEGEADSCHDEGVVKEIPITHHVKEGYEKADPAQFELLKVLGQGSFGKVFLVRKKTGPDAGQLYAMKVLKKASLKVRDRVRTKMERDILVEVNHPFIVKLHYAFQTEGKLYLILDFLRGGDVFTRLSKEVLFTEEDVKFYLAELALALDHLHQLGIVYRDLKPENILLDEIGHIKLTDFGLSKESVDQEKKAYSFCGTVEYMAPEVVNRRGHSQSADWWSYGVLMFEMLTGTLPFQGKDRNETMNMILKAKLGMPQFLSAEAQSLLRMLFKRNPANRLGSEGVEEIKRHLFFANIDWDKLYKREVQPPFKPASGKPDDTFCFDPEFTAKTPKDSPGLPASANAHQLFKGFSFVATSIAEEYKITPITSANVLPIVQINGNAAQFGEVYELKEDIGVGSYSVCKRCIHATTNMEFAVKIIDKSKRDPSEEIEILMRYGQHPNIITLKDVFDDGRYVYLVTDLMKGGELLDRILKQKCFSEREASDILYVISKTVDYLHCQGVVHRDLKPSNILYMDESASADSIRICDFGFAKQLRGENGLLLTPCYTANFVAPEVLMQQGYDAACDIWSLGVLFYTMLAGYTPFANGPNDTPEEILLRIGNGKFSLSGGNWDNISDGAKDLLSHMLHMDPHQRYTAEQILKHSWITHRDQLPNDQPKRNDVSHVVKGAMVATYSALTHKTFQPVLEPVAASSLAQRRSMKKRTSTGL

PTMs - Q15418/P51812/Q15349/Q9UK32 基板として

Site PTM Type Enzyme
S26 Phosphorylation
S27 Phosphorylation
S28 Phosphorylation
S30 Phosphorylation
K91 Acetylation
K94 Acetylation
S106 Phosphorylation
Y135 Phosphorylation
K186 Ubiquitination
S206 Phosphorylation
Y217 Phosphorylation
S218 Phosphorylation O15530 (PDPK1) , Q15349 (RPS6KA2)
T222 Phosphorylation
K295 Acetylation
T356 Phosphorylation
S360 Phosphorylation
S377 Phosphorylation
S381 Phosphorylation
S388 Phosphorylation
Y426 Phosphorylation
K430 Ubiquitination
K450 Ubiquitination
Y463 Phosphorylation
T471 Phosphorylation
K473 Ubiquitination
S546 Phosphorylation
T570 Phosphorylation Q15349 (RPS6KA2)
Y573 Phosphorylation
T574 Phosphorylation
T618 Phosphorylation
S628 Phosphorylation
K630 Ubiquitination
S650 Phosphorylation
T662 Phosphorylation
S730 Phosphorylation
Site PTM Type Enzyme
S17 Phosphorylation
S19 Phosphorylation
K57 Ubiquitination
K62 Ubiquitination
K72 Ubiquitination
S78 Phosphorylation
K81 Acetylation
K100 Acetylation
K103 Acetylation
Y144 Phosphorylation
K195 Ubiquitination
S215 Phosphorylation
K216 Ubiquitination
Y226 Phosphorylation
S227 Phosphorylation P51812 (RPS6KA3) , P27361 (MAPK3) , O15530 (PDPK1)
T231 Phosphorylation P51812 (RPS6KA3)
Y234 Phosphorylation
K274 Ubiquitination
T278 Phosphorylation
K322 Ubiquitination
K345 Ubiquitination
Y356 Phosphorylation
T362 Phosphorylation
K364 Ubiquitination
T365 Phosphorylation P51812 (RPS6KA3)
S369 Phosphorylation P51812 (RPS6KA3)
S375 Phosphorylation
S386 Phosphorylation P28482 (MAPK1) , P51812 (RPS6KA3) , P49137 (MAPKAPK2) , O15530 (PDPK1)
T391 Phosphorylation
S392 Phosphorylation
S396 Phosphorylation
S415 Phosphorylation
Y433 Phosphorylation
K437 Ubiquitination
Y470 Phosphorylation P12931 (SRC) , P06241 (FYN)
T478 Phosphorylation
K480 Ubiquitination
Y483 Phosphorylation P06241 (FYN) , P12931 (SRC)
Y488 Phosphorylation P12931 (SRC) , P06241 (FYN) , P28482 (MAPK1) , P22607 (FGFR3)
K504 Ubiquitination
K509 Ubiquitination
S517 Phosphorylation
Y529 Phosphorylation P12931 (SRC) , P28482 (MAPK1) , P22607 (FGFR3) , P06241 (FYN)
K541 Ubiquitination
S556 Phosphorylation
K566 Ubiquitination
T573 Phosphorylation
T577 Phosphorylation P27361 (MAPK3)
Y580 Phosphorylation P06241 (FYN) , P12931 (SRC)
T581 Phosphorylation P51812 (RPS6KA3)
K591 Ubiquitination
T625 Phosphorylation
Y644 Phosphorylation P12931 (SRC) , P06241 (FYN)
K658 Ubiquitination
Y688 Phosphorylation
T706 Phosphorylation
Y707 Phosphorylation P06241 (FYN) , P35916 (FLT4) , P11362 (FGFR1) , P22607 (FGFR3) , P12931 (SRC)
S708 Phosphorylation
S715 Phosphorylation P51812 (RPS6KA3)
R723 Methylation
S737 Phosphorylation
Site PTM Type Enzyme
S45 Phosphorylation
T47 Phosphorylation
K51 Ubiquitination
S54 Phosphorylation
K56 Ubiquitination
K66 Ubiquitination
S72 Phosphorylation
K75 Acetylation
K75 Ubiquitination
K81 Ubiquitination
Y91 Phosphorylation
K94 Ubiquitination
Y138 Phosphorylation
S154 Phosphorylation
K189 Ubiquitination
S209 Phosphorylation
K210 Ubiquitination
Y220 Phosphorylation
S221 Phosphorylation Q15418 (RPS6KA1) , O15530 (PDPK1)
T225 Phosphorylation
Y228 Phosphorylation
K278 Ubiquitination
S307 Phosphorylation
K316 Ubiquitination
T359 Phosphorylation
S363 Phosphorylation
S369 Phosphorylation
S380 Phosphorylation P49137 (MAPKAPK2) , P41279 (MAP3K8) , Q13237 (PRKG2) , Q15418 (RPS6KA1)
T384 Phosphorylation
K392 Ubiquitination
S402 Phosphorylation
K410 Ubiquitination
K421 Ubiquitination
K433 Ubiquitination
K438 Ubiquitination
K453 Ubiquitination
Y466 Phosphorylation
T474 Phosphorylation
K476 Ubiquitination
Y486 Phosphorylation
T489 Phosphorylation
K500 Ubiquitination
K505 Ubiquitination
S513 Phosphorylation
T522 Phosphorylation
K537 Ubiquitination
K562 Ubiquitination
T573 Phosphorylation P28482 (MAPK1)
Y576 Phosphorylation
T577 Phosphorylation
K587 Ubiquitination
K633 Ubiquitination
S637 Phosphorylation
K649 Ubiquitination
K654 Ubiquitination
K667 Ubiquitination
K678 Ubiquitination
K680 Ubiquitination
S684 Phosphorylation
S687 Phosphorylation
T701 Phosphorylation
Y702 Phosphorylation
S703 Phosphorylation
K709 Ubiquitination
S732 Phosphorylation
T733 Phosphorylation
T734 Phosphorylation
Site PTM Type Enzyme
S83 Phosphorylation
K86 Acetylation
K86 Ubiquitination
T94 Phosphorylation
K105 Acetylation
K108 Acetylation
Y149 Phosphorylation
S220 Phosphorylation
Y231 Phosphorylation
S232 Phosphorylation
T236 Phosphorylation
Y239 Phosphorylation
K348 Acetylation
K367 Acetylation
S372 Phosphorylation
S378 Phosphorylation
K386 Acetylation
S389 Phosphorylation
Y437 Phosphorylation
K441 Ubiquitination
Y474 Phosphorylation
T482 Phosphorylation
S521 Phosphorylation
Y525 Phosphorylation
S547 Phosphorylation
S555 Phosphorylation
K570 Ubiquitination
T581 Phosphorylation
T710 Phosphorylation
Y711 Phosphorylation
T715 Phosphorylation
T718 Phosphorylation
S730 Phosphorylation
S736 Phosphorylation

PTMs - Q15418/P51812/Q15349/Q9UK32 酵素として

Substrate Site Source
P16220 (CREB1) S133 Uniprot
P17676 (CEBPB) S321 Uniprot
P25963 (NFKBIA) S32 Uniprot
P32004-2 (L1CAM) S1152 Uniprot
Q15349-1 (RPS6KA2) S218 Uniprot
Q15349-1 (RPS6KA2) T222 Uniprot
Q15349 (RPS6KA2) T570 Uniprot
Q92934 (BAD) S75 Uniprot
Q92934 (BAD) S118 Uniprot
Substrate Site Source
O14649 (KCNK3) S393 Uniprot
O60825 (PFKFB2) S466 Uniprot
P03372 (ESR1) S167 Uniprot
P04637 (TP53) S15 Uniprot
P04792 (HSPB1) S78 Uniprot
P04792 (HSPB1) S82 Uniprot
P05114 (HMGN1) S7 Uniprot
P05114 (HMGN1) S21 Uniprot
P05114 (HMGN1) S25 Uniprot
P05198 (EIF2S1) S52 Uniprot
P05204 (HMGN2) S25 Uniprot
P05204 (HMGN2) S29 Uniprot
P05783 (KRT18) S53 Uniprot
P07101 (TH) S40 Uniprot
P07101-4 (TH) S44 Uniprot
P07101-2 (TH) S67 Uniprot
P07101 (TH) S71 Uniprot
P10636-6 (MAPT) T154 Uniprot
P10636-8 (MAPT) T212 Uniprot
P11362 (FGFR1) S789 Uniprot
P13796 (LCP1) S5 Uniprot
P16220 (CREB1) S133 Uniprot
P17676 (CEBPB) S321 Uniprot
P18846 (ATF1) S63 Uniprot
P18848 (ATF4) S245 Uniprot
P19525 (EIF2AK2) T451 Uniprot
P22736-1 (NR4A1) S351 Uniprot
P23588 (EIF4B) S422 Uniprot
P25963 (NFKBIA) S32 Uniprot
P29317 (EPHA2) S897 Uniprot
P33778 (HIST1H2BB) S33 Uniprot
P40763 (STAT3) S727 Uniprot
P46527 (CDKN1B) T198 Uniprot
P48764 (SLC9A3) S663 Uniprot
P49815 (TSC2) S1798 Uniprot
P49840 (GSK3A) S21 Uniprot
P49841 (GSK3B) S9 Uniprot
P51812 (RPS6KA3) S227 Uniprot
P51812 (RPS6KA3) T231 Uniprot
P51812 (RPS6KA3) T365 Uniprot
P51812 (RPS6KA3) S369 Uniprot
P51812 (RPS6KA3) S386 Uniprot
P51812 (RPS6KA3) T581 Uniprot
P51812 (RPS6KA3) S715 Uniprot
P53355 (DAPK1) S289 Uniprot
P62753 (RPS6) S235 Uniprot
P62753 (RPS6) S236 Uniprot
P67809 (YBX1) S102 Uniprot
P68431 (HIST1H3J) S11 Uniprot
P84243 (H3F3B) S11 Uniprot
Q04206 (RELA) S536 Uniprot
Q06413 (MEF2C) S192 Uniprot
Q14790 (CASP8) T263 Uniprot
Q14934 (NFATC4) S281 Uniprot
Q14934 (NFATC4) S285 Uniprot
Q14934 (NFATC4) S289 Uniprot
Q14934 (NFATC4) S344 Uniprot
Q14934 (NFATC4) S676 Uniprot
Q15831-1 (STK11) S428 Uniprot
Q16695 (HIST3H3) S11 Uniprot
Q16821 (PPP1R3A) S46 Uniprot
Q16821 (PPP1R3A) S65 Uniprot
Q8IX03 (WWC1) T929 Uniprot
Q8IX03 (WWC1) S947 Uniprot
Q8N122 (RPTOR) S719 Uniprot
Q8N122 (RPTOR) S721 Uniprot
Q8N122 (RPTOR) S722 Uniprot
Q92934 (BAD) S75 Uniprot
Q92934 (BAD) S99 Uniprot
Q96RK0 (CIC) S173 Uniprot
Q99683 (MAP3K5) S83 Uniprot
Q99683 (MAP3K5) T1109 Uniprot
Q99683 (MAP3K5) T1326 Uniprot
Q9BSI4 (TINF2) S295 Uniprot
Q9BSI4 (TINF2) S330 Uniprot
Q9H6Z4 (RANBP3) S126 Uniprot
Q9NYV6 (RRN3) S649 Uniprot
Substrate Site Source
O00418 (EEF2K) S366 Uniprot
O14727 (APAF1) S268 Uniprot
O14727 (APAF1) S357 Uniprot
O14757 (CHEK1) S280 Uniprot
O60343 (TBC1D4) S341 Uniprot
O60343 (TBC1D4) S751 Uniprot
O75030-9 (MITF) S409 Uniprot
P01100 (FOS) S362 Uniprot
P03372-1 (ESR1) S118 Uniprot
P03372-1 (ESR1) S167 Uniprot
P11831 (SRF) S103 Uniprot
P13796 (LCP1) S5 Uniprot
P16144 (ITGB4) S1364 Uniprot
P16220-1 (CREB1) S133 Uniprot
P17676 (CEBPB) T235 Uniprot
P17676-1 (CEBPB) T266 Uniprot
P19634 (SLC9A1) S703 Uniprot
P21333 (FLNA) S2152 Uniprot
P22736-1 (NR4A1) S351 Uniprot
P23588 (EIF4B) S422 Uniprot
P25963 (NFKBIA) S32 Uniprot
P25963 (NFKBIA) S36 Uniprot
P29317 (EPHA2) S897 Uniprot
P30304 (CDC25A) S293 Uniprot
P30304 (CDC25A) S295 Uniprot
P30305 (CDC25B) S353 Uniprot
P30305 (CDC25B) T355 Uniprot
P30307 (CDC25C) S247 Uniprot
P32004-1 (L1CAM) S1152 Uniprot
P42345 (MTOR) T2446 Uniprot
P42345 (MTOR) S2448 Uniprot
P43354-1 (NR4A2) S347 Uniprot
P46527 (CDKN1B) T198 Uniprot
P49427 (CDC34) T162 Uniprot
P49815 (TSC2) S1798 Uniprot
P49841 (GSK3B) S9 Uniprot
P50549 (ETV1) S191 Uniprot
P50549 (ETV1) S216 Uniprot
P50549-1 (ETV1) S334 Uniprot
P50552 (VASP) T278 Uniprot
P53355 (DAPK1) S289 Uniprot
P60983 (GMFB) T27 Uniprot
P62753 (RPS6) S235 Uniprot
P62753 (RPS6) S236 Uniprot
P62753 (RPS6) S240 Uniprot
P62753 (RPS6) S244 Uniprot
P67809 (YBX1) S102 Uniprot
P78371 (CCT2) S260 Uniprot
P84243 (H3F3B) S11 Uniprot
Q04206 (RELA) S536 Uniprot
Q05195 (MXD1) S145 Uniprot
Q07889 (SOS1) S1134 Uniprot
Q07889 (SOS1) S1161 Uniprot
Q13164 (MAPK7) S496 Uniprot
Q15418 (RPS6KA1) S221 Uniprot
Q15418-1 (RPS6KA1) T225 Uniprot
Q15418-2 (RPS6KA1) T234 Uniprot
Q15418-1 (RPS6KA1) S380 Uniprot
Q15418-2 (RPS6KA1) S389 Uniprot
Q15418-1 (RPS6KA1) T577 Uniprot
Q15418-2 (RPS6KA1) T586 Uniprot
Q15653 (NFKBIB) S19 Uniprot
Q15653 (NFKBIB) S23 Uniprot
Q15831 (STK11) S428 Uniprot
Q16821-1 (PPP1R3A) S46 Uniprot
Q16875 (PFKFB3) S461 Uniprot
Q8IX03 (WWC1) T929 Uniprot
Q8IX03 (WWC1) S947 Uniprot
Q8N122 (RPTOR) S719 Uniprot
Q8N122 (RPTOR) S721 Uniprot
Q8N122 (RPTOR) S722 Uniprot
Q8TB45 (DEPTOR) S286 Uniprot
Q8TB45 (DEPTOR) S287 Uniprot
Q8TB45 (DEPTOR) S291 Uniprot
Q92570 (NR4A3) S376 Uniprot
Q92570-3 (NR4A3) S387 Uniprot
Q92882 (OSTF1) S202 Uniprot
Q92934 (BAD) S75 Uniprot
Q92934 (BAD) S99 Uniprot
Q92934 (BAD) S118 Uniprot
Q92934 (BAD) S153 Uniprot
Q96RK0 (CIC) S173 Uniprot
Q9H6Z4 (RANBP3) S126 Uniprot
Q9HC62 (SENP2) T369 Uniprot
Q9UBP6-1 (METTL1) S27 Uniprot
Q9UN36 (NDRG2) S332 Uniprot
Q9UN36 (NDRG2) S350 Uniprot
Q9Y2V2 (CARHSP1) S52 Uniprot
Substrate Site Source
Q15831 (STK11) S428 Uniprot

研究背景

機能:

Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1, which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP. Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity. Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the pre-initiation complex. In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation. Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at 'Ser-1798', which potently inhibits TSC2 ability to suppress mTOR signaling, and mediates phosphorylation of RPTOR, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway. Mediates cell survival by phosphorylating the pro-apoptotic proteins BAD and DAPK1 and suppressing their pro-apoptotic function. Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4). Mediates induction of hepatocyte prolifration by TGFA through phosphorylation of CEBPB (By similarity). Is involved in cell cycle regulation by phosphorylating the CDK inhibitor CDKN1B, which promotes CDKN1B association with 14-3-3 proteins and prevents its translocation to the nucleus and inhibition of G1 progression. Phosphorylates EPHA2 at 'Ser-897', the RPS6KA-EPHA2 signaling pathway controls cell migration.

PTMs:

Activated by phosphorylation at Ser-221 by PDPK1. Autophosphorylated on Ser-380, as part of the activation process. May be phosphorylated at Thr-359 and Ser-363 by MAPK1/ERK2 and MAPK3/ERK1.

N-terminal myristoylation results in an activated kinase in the absence of added growth factors.

細胞の位置付け:

Nucleus. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Forms a complex with either MAPK1/ERK2 or MAPK3/ERK1 in quiescent cells. Transiently dissociates following mitogenic stimulation. Interacts with ETV1/ER81 and FGFR1.

タンパク質ファミリー:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.

機能:

Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1 and histone H3 at 'Ser-10', which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP. Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity. Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the preinitiation complex. In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation. Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at 'Ser-1798', which potently inhibits TSC2 ability to suppress mTOR signaling, and mediates phosphorylation of RPTOR, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway. Mediates cell survival by phosphorylating the pro-apoptotic proteins BAD and DAPK1 and suppressing their pro-apoptotic function. Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4). Is involved in cell cycle regulation by phosphorylating the CDK inhibitor CDKN1B, which promotes CDKN1B association with 14-3-3 proteins and prevents its translocation to the nucleus and inhibition of G1 progression. In LPS-stimulated dendritic cells, is involved in TLR4-induced macropinocytosis, and in myeloma cells, acts as effector of FGFR3-mediated transformation signaling, after direct phosphorylation at Tyr-529 by FGFR3. Negatively regulates EGF-induced MAPK1/3 phosphorylation via phosphorylation of SOS1. Phosphorylates SOS1 at 'Ser-1134' and 'Ser-1161' that create YWHAB and YWHAE binding sites and which contribute to the negative regulation of MAPK1/3 phosphorylation (By similarity). Phosphorylates EPHA2 at 'Ser-897', the RPS6KA-EPHA2 signaling pathway controls cell migration.

PTMs:

Activated by phosphorylation at Ser-227 by PDPK1. Autophosphorylated on Ser-386, as part of the activation process. May be phosphorylated at Thr-365 and Ser-369 by MAPK1/ERK2 and MAPK3/ERK1. Can also be activated via phosphorylation at Ser-386 by MAPKAPK2.

N-terminal myristoylation results in an activated kinase in the absence of added growth factors.

細胞の位置付け:

Nucleus. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in many tissues, highest levels in skeletal muscle.

サブユニット構造:

Forms a complex with either MAPK1/ERK2 or MAPK3/ERK1 in quiescent cells. Transiently dissociates following mitogenic stimulation (By similarity). Interacts with NFATC4, ETV1/ER81 and FGFR1.

タンパク質ファミリー:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.

機能:

Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of transcription factors, regulates translation, and mediates cellular proliferation, survival, and differentiation. May function as tumor suppressor in epithelial ovarian cancer cells.

PTMs:

Activated by phosphorylation at Ser-218 by PDPK1. Autophosphorylated on Ser-377, as part of the activation process. May be phosphorylated at Thr-356 and Ser-360 by MAPK1/ERK2 and MAPK3/ERK1 (By similarity).

N-terminal myristoylation results in an activated kinase in the absence of added growth factors.

細胞の位置付け:

Nucleus. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Widely expressed with higher expression in lung, skeletal muscle, brain, uterus, ovary, thyroid and prostate.

サブユニット構造:

Forms a complex with either MAPK1/ERK2 or MAPK3/ERK1 in quiescent cells. Transiently dissociates following mitogenic stimulation (By similarity). Interacts with FBXO5; cooperate to induce the metaphase arrest of early blastomeres; increases and stabilizes interaction of FBXO5 with CDC20 (By similarity).

タンパク質ファミリー:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.

機能:

Constitutively active serine/threonine-protein kinase that exhibits growth-factor-independent kinase activity and that may participate in p53/TP53-dependent cell growth arrest signaling and play an inhibitory role during embryogenesis.

PTMs:

Phosphorylated at Ser-232, Ser-372, and Ser-389 in serum-starved cells.

細胞の位置付け:

Cytoplasm>Cytosol. Nucleus.
Note: Predominantly cytosolic.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Forms a complex with MAPK3/ERK1 but not with MAPK9 or MAPK14 in serum-starved cells.

タンパク質ファミリー:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.

研究領域

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Organismal Systems > Nervous system > Long-term potentiation.

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

参考文献

1). Activation of RSK2 upregulates SOX8 to promote methotrexate resistance in gestational trophoblastic neoplasia. LABORATORY INVESTIGATION, 2021 (PubMed: 34373588) [IF=5.0]

Application: WB    Species: Human    Sample: JAR/MTX and JAR cell

Fig. 1Quantitative phosphoproteomic analysis on JAR/MTX and JAR cell models.

Application: IHC    Species: Human    Sample: GTN

Fig. 6RSK2/SOX8 signaling was highly enriched in MTX-resistant GTN samples. A Immunostaining of p-RSK and SOX8 in GTN samples. Representative IHC staining micrographs were shown for MTX-resistant (left panel) or MTX-sensitive (right panel) GTNs. Bars: 100 µm. B p-RSK and SOX8 were highly expressed in MTX-resistant GTN specimens. *P < 0.05; **P < 0.01. C High expression of p-RSK was significantly correlated with high expression of SOX8 in GTN specimens.

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