Phospho-ICK (Tyr159) Antibody - #AF3816
| 製品: | Phospho-ICK (Tyr159) Antibody |
| カタログ: | AF3816 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-ICK (Tyr159) |
| アプリケーション: | WB ELISA(peptide) |
| 反応性: | Human, Mouse, Rat |
| 分子量: | 71kD(Calculated). |
| ユニプロット: | Q9UPZ9 |
| RRID: | AB_2847130 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF3816, RRID:AB_2847130.
折りたたみ／展開
2210420N10Rik; AI848300; ECO; Heart serine threonine protein kinase; hICK; Intestinal cell (MAK like) kinase; Intestinal cell kinase; KIAA0936; Laryngeal cancer kinase 2; LCK2; MAK related kinase; MGC46090; mICK; MRK; OTTMUSP00000041856; OTTMUSP00000041857; Serine/threonine protein kinase ICK;
免疫原
A synthesized peptide derived from human ICK around the phosphorylation site of Tyr159.
Expressed in heart, brain, placenta, pancreas, thymus, prostate, testis, ovary, small intestine and colon, with highest levels in placenta and testis. Not detected in spleen. Also expressed in many cancer cell lines.
- Q9UPZ9 CILK1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNRYTTIRQLGDGTYGSVLLGRSIESGELIAIKKMKRKFYSWEECMNLREVKSLKKLNHANVVKLKEVIRENDHLYFIFEYMKENLYQLIKERNKLFPESAIRNIMYQILQGLAFIHKHGFFHRDLKPENLLCMGPELVKIADFGLAREIRSKPPYTDYVSTRWYRAPEVLLRSTNYSSPIDVWAVGCIMAEVYTLRPLFPGASEIDTIFKICQVLGTPKKTDWPEGYQLSSAMNFRWPQCVPNNLKTLIPNASSEAVQLLRDMLQWDPKKRPTASQALRYPYFQVGHPLGSTTQNLQDSEKPQKGILEKAGPPPYIKPVPPAQPPAKPHTRISSRQHQASQPPLHLTYPYKAEVSRTDHPSHLQEDKPSPLLFPSLHNKHPQSKITAGLEHKNGEIKPKSRRRWGLISRSTKDSDDWADLDDLDFSPSLSRIDLKNKKRQSDDTLCRFESVLDLKPSEPVGTGNSAPTQTSYQRRDTPTLRSAAKQHYLKHSRYLPGISIRNGILSNPGKEFIPPNPWSSSGLSGKSSGTMSVISKVNSVGSSSTSSSGLTGNYVPSFLKKEIGSAMQRVHLAPIPDPSPGYSSLKAMRPHPGRPFFHTQPRSTPGLIPRPPAAQPVHGRTDWASKYASRR
PTMs - Q9UPZ9 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T6 | Phosphorylation | Uniprot | |
| T14 | Phosphorylation | Uniprot | |
| Y15 | Phosphorylation | Uniprot | |
| S17 | Phosphorylation | Uniprot | |
| S23 | Phosphorylation | Uniprot | |
| S53 | Phosphorylation | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| Y156 | Phosphorylation | Uniprot | |
| T157 | Phosphorylation | P50613 (CDK7) , Q8IZL9 (CDK20) | Uniprot |
| Y159 | Phosphorylation | Q9UPZ9 (ICK) | Uniprot |
| Y177 | Phosphorylation | Uniprot | |
| T248 | Phosphorylation | Uniprot | |
| S254 | Phosphorylation | Uniprot | |
| T463 | Phosphorylation | Uniprot | |
| T478 | Phosphorylation | Uniprot | |
| K491 | Methylation | Uniprot | |
| S520 | Phosphorylation | Uniprot | |
| S528 | Phosphorylation | Uniprot | |
| S529 | Phosphorylation | Uniprot | |
| K562 | Acetylation | Uniprot | |
| Y583 | Phosphorylation | Uniprot | |
| S585 | Phosphorylation | Uniprot |
PTMs - Q9UPZ9 酵素として
研究背景
Required for ciliogenesis. Phosphorylates KIF3A (By similarity). Involved in the control of ciliary length. Regulates the ciliary localization of SHH pathway components as well as the localization of IFT components at ciliary tips (By similarity). May play a key role in the development of multiple organ systems and particularly in cardiac development (By similarity). Regulates intraflagellar transport (IFT) speed and negatively regulates cilium length in a cAMP and mTORC1 signaling-dependent manner and this regulation requires its kinase activity (By similarity).
Autophosphorylated on serine and threonine residues. Phosphorylation at Thr-157 increases kinase activity.
Nucleus. Cytoplasm>Cytosol. Cell projection>Cilium. Cytoplasm>Cytoskeleton>Cilium basal body.
Note: Also found at the ciliary tip (PubMed:24797473). Nuclear localization has been observed with a GFP-tagged construct in transfected HeLa cells (PubMed:12103360, PubMed:19185282).
Cytoplasm.
Note: Predominant cytoplasmic localization has been observed with a N-terminally GFP-tagged construct.
Expressed in heart, brain, placenta, pancreas, thymus, prostate, testis, ovary, small intestine and colon, with highest levels in placenta and testis. Not detected in spleen. Also expressed in many cancer cell lines.
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
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