Phospho-4E-BP1 (Thr37/Thr46) Antibody - #AF3830

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製品: Phospho-4E-BP1 (Thr37/Thr46) Antibody
カタログ: AF3830
タンパク質の説明: Rabbit polyclonal antibody to Phospho-4E-BP1 (Thr37/Thr46)
アプリケーション: WB IHC IF/ICC
Cited expt.: WB, IF/ICC
反応性: Human, Mouse, Rat
分子量: 17kD; 13kD(Calculated).
ユニプロット: Q13541
RRID: AB_2847144

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
Phospho-4E-BP1 (Thr37/Thr46) Antibody detects endogenous levels of 4E-BP1 only when phosphorylated at Thr37/46.
RRID:
AB_2847144
引用形式: Affinity Biosciences Cat# AF3830, RRID:AB_2847144.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

4E-BP1; 4EBP1; 4EBP1_HUMAN; BP 1; eIF4E binding protein 1; eIF4E-binding protein 1; Eif4ebp1; Eukaryotic translation initiation factor 4E-binding protein 1; PHAS-I; PHASI; Phosphorylated heat- and acid-stable protein regulated by insulin 1;

免疫原

免疫原:

A synthesized peptide derived from human 4E-BP1 around the phosphorylation site of Thr37/46.

Uniprot:

Q13541(4EBP1_HUMAN) >>Visit HPA database.

遺伝子(ID):
EIF4EBP1(1978)
タンパク質配列:
MSGGSSCSQTPSRAIPATRRVVLGDGVQLPPGDYSTTPGGTLFSTTPGGTRIIYDRKFLMECRNSPVTKTPPRDLPTIPGVTSPSSDEPPMEASQSHLRNSPEDKRAGGEESQFEMDI

研究背景

機能:

Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.

PTMs:

Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70, corresponding to the hyperphosphorylated form, is regulated by mTORC1 and abolishes binding to EIF4E.

Ubiquitinated: when eIF4E levels are low, hypophosphorylated form is ubiquitinated by the BCR(KLHL25) complex, leading to its degradation and serving as a homeostatic mechanism to maintain translation and prevent eIF4E inhibition when eIF4E levels are low. Not ubiquitinated when hyperphosphorylated (at Thr-37, Thr-46, Ser-65 and Thr-70) or associated with eIF4E.

タンパク質ファミリー:

The TOS motif mediates interaction with RPTOR, leading to promote phosphorylation by mTORC1 complex.

Belongs to the eIF4E-binding protein family.

研究領域

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Genetic Information Processing > Translation > RNA transport.

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Overview > Choline metabolism in cancer.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

参考文献

1). Identification and analysis of microplastic aggregation in CAR-T cells. Journal of hazardous materials, 2024 (PubMed: 39488976) [IF=13.6]
2). Kaempferol Improves Exercise Performance by Regulating Glucose Uptake, Mitochondrial Biogenesis, and Protein Synthesis via PI3K/AKT and MAPK Signaling Pathways. Foods (Basel, Switzerland), 2024 (PubMed: 38611372) [IF=5.2]

Application: IF/ICC    Species: Mouse    Sample:

Figure 6 Protein expression/phosphorylation of downstream maker targeted to biological functions in C2C12 myotubes. Protein expression of mtTFA (A) and GLUT4 (B) and the phosphorylation of 4EBP1 (C) and p70S6K (D) were detected with a high-content imaging analysis system. Left panel: representative images, bar size: 50 μm. The assays were performed in triplicate with three technical replicates.
3). Protective effects and potential mechanisms of (2-Carboxyethyl) dimethylsulfonium Bromide (Br-DMPT) on gill health status of on-growing grass carp (Ctenopharyngodon idella) after infection with Flavobacterium columnare. FISH & SHELLFISH IMMUNOLOGY, 2020 (PubMed: 32771611) [IF=4.1]

Application: WB    Species: grass carp    Sample: gill

Fig. 8. |Western blot analysis of TOR protein phosphorylation (p-TOR Ser2448) (A), S6K1 protein phosphorylation (p-S6K1 Thr-389) (B), 4EBP1 protein phosphorylation (Thr-37/46) (C) in the gill of on-growing grass carp fed diets containing graded levels of Br-DMPT for 60 days, then infected with F. columnare for 3 days.Data represent means of six fish in each group, error bars indicate S.D. Values having different letters are significantly different (P < 0.05).
4). Rapamycin inhibits B16 melanoma cell viability invitro and invivo by inducing autophagy and inhibiting the mTOR/p70‑S6k pathway. Oncology letters, 2024 (PubMed: 38385108) [IF=2.5]

Application: WB    Species: Mouse    Sample: B16 cells

Figure 5. RAPA inhibits the mTOR/p70-S6k signaling pathway in B16 cells. B16 cells were treated with 0, 1, 10 and 100 nM RAPA for 48 h. (A) Western blotting results showed that RAPA inhibited the expression of p-mTOR and p-p70-S6k compared with in the control group. Statistical analysis of the (B) p-mTOR/mTOR ratio, (C) the p-p70-S6k/p70-S6k ratio and (D) the p-4E-BP1/4E-BP1 ratio at the protein level. Data are presented as the mean ± SD (n=3). *P
5). L-Leucine stimulates proliferation and casein secretion in porcine mammary epithelial cells through mTOR-p70S6K-4EBP1 signalling pathway. , 2022

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