Phospho-4E-BP1 (Thr37/Thr46) Antibody - #AF3830
(4)
(22)
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF3830, RRID:AB_2847144.
折りたたみ／展開
4E-BP1; 4EBP1; 4EBP1_HUMAN; BP 1; eIF4E binding protein 1; eIF4E-binding protein 1; Eif4ebp1; Eukaryotic translation initiation factor 4E-binding protein 1; PHAS-I; PHASI; Phosphorylated heat- and acid-stable protein regulated by insulin 1;
免疫原
A synthesized peptide derived from human 4E-BP1 around the phosphorylation site of Thr37/46.
- Q13541 4EBP1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSGGSSCSQTPSRAIPATRRVVLGDGVQLPPGDYSTTPGGTLFSTTPGGTRIIYDRKFLMECRNSPVTKTPPRDLPTIPGVTSPSSDEPPMEASQSHLRNSPEDKRAGGEESQFEMDI
PTMs - Q13541 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | Uniprot | |
| S5 | Phosphorylation | Uniprot | |
| S6 | Phosphorylation | Uniprot | |
| S8 | Phosphorylation | Q13315 (ATM) | Uniprot |
| T10 | Phosphorylation | Uniprot | |
| S12 | Phosphorylation | Uniprot | |
| Y34 | Phosphorylation | Uniprot | |
| S35 | Phosphorylation | Uniprot | |
| T36 | Phosphorylation | P51449 (RORC) , O75582 (RPS6KA5) , P31751 (AKT2) , P42345 (MTOR) | Uniprot |
| T37 | Phosphorylation | P49841 (GSK3B) , Q16539 (MAPK14) , P31749 (AKT1) , P42345 (MTOR) , P06493 (CDK1) , P28482 (MAPK1) , Q5S007 (LRRK2) | Uniprot |
| T41 | Phosphorylation | P42345 (MTOR) , P49674 (CSNK1E) | Uniprot |
| S44 | Phosphorylation | P42345 (MTOR) | Uniprot |
| T45 | Phosphorylation | O75582 (RPS6KA5) , P28482 (MAPK1) , P42345 (MTOR) , P31751 (AKT2) , P51449 (RORC) | Uniprot |
| T46 | Phosphorylation | P06493 (CDK1) , Q5S007 (LRRK2) , Q16539 (MAPK14) , P42345 (MTOR) , P31749 (AKT1) , P28482 (MAPK1) , P49841 (GSK3B) | Uniprot |
| T50 | Phosphorylation | P49674 (CSNK1E) | Uniprot |
| Y54 | Phosphorylation | Uniprot | |
| K57 | Ubiquitination | Uniprot | |
| C62 | S-Nitrosylation | Uniprot | |
| R63 | Methylation | Uniprot | |
| S65 | Phosphorylation | P27361 (MAPK3) , P42345 (MTOR) , P28482 (MAPK1) , O75582 (RPS6KA5) , Q8TAI7 (RHEBL1) , P31749 (AKT1) , Q92630 (DYRK2) , Q9P1W9 (PIM2) , P06493 (CDK1) , Q16539 (MAPK14) | Uniprot |
| T68 | Phosphorylation | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| T70 | Phosphorylation | P28482 (MAPK1) , P06493 (CDK1) , Q16539 (MAPK14) , P42345 (MTOR) , P41279 (MAP3K8) | Uniprot |
| T77 | Phosphorylation | Uniprot | |
| T82 | Phosphorylation | Uniprot | |
| S83 | Phosphorylation | P42345 (MTOR) , P06493 (CDK1) , P28482 (MAPK1) | Uniprot |
| S85 | Phosphorylation | Uniprot | |
| S86 | Phosphorylation | Uniprot | |
| S94 | Phosphorylation | Q13315 (ATM) , Q13535 (ATR) | Uniprot |
| S96 | Phosphorylation | Uniprot | |
| S101 | Phosphorylation | Q92630 (DYRK2) , P42345 (MTOR) | Uniprot |
| S112 | Phosphorylation | P19784 (CSNK2A2) , P68400 (CSNK2A1) , Q13315 (ATM) | Uniprot |
研究背景
Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70, corresponding to the hyperphosphorylated form, is regulated by mTORC1 and abolishes binding to EIF4E.
Ubiquitinated: when eIF4E levels are low, hypophosphorylated form is ubiquitinated by the BCR(KLHL25) complex, leading to its degradation and serving as a homeostatic mechanism to maintain translation and prevent eIF4E inhibition when eIF4E levels are low. Not ubiquitinated when hyperphosphorylated (at Thr-37, Thr-46, Ser-65 and Thr-70) or associated with eIF4E.
Hypophosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) or mTORC1 phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Interacts (via TOS motif) with RPTOR; promoting phosphorylation by mTORC1.
The TOS motif mediates interaction with RPTOR, leading to promote phosphorylation by mTORC1 complex.
Belongs to the eIF4E-binding protein family.
研究領域
· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)
· Environmental Information Processing > Signal transduction > ErbB signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)
· Genetic Information Processing > Translation > RNA transport.
· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Human Diseases > Cancers: Specific types > Acute myeloid leukemia. (View pathway)
· Human Diseases > Cancers: Overview > Choline metabolism in cancer. (View pathway)
· Organismal Systems > Aging > Longevity regulating pathway. (View pathway)
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
参考文献
Application: IF/ICC Species: Mouse Sample:
Application: WB Species: grass carp Sample: gill
Application: WB Species: Mouse Sample: B16 cells
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