Phospho-IRF7 (Ser471/Ser472) Antibody - #AF3885
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF3885, RRID:AB_2847199.
折りたたみ／展開
IMD39; Interferon regulatory factor 7; Interferon regulatory factor 7H; IRF 7; IRF 7A; IRF 7H; IRF-7; IRF7; IRF7_HUMAN; IRF7A; IRF7B; IRF7C; IRF7H;
免疫原
A synthesized peptide derived from human IRF7 around the phosphorylation site of Ser471/472.
- Q92985 IRF7_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MALAPERAAPRVLFGEWLLGEISSGCYEGLQWLDEARTCFRVPWKHFARKDLSEADARIFKAWAVARGRWPPSSRGGGPPPEAETAERAGWKTNFRCALRSTRRFVMLRDNSGDPADPHKVYALSRELCWREGPGTDQTEAEAPAAVPPPQGGPPGPFLAHTHAGLQAPGPLPAPAGDKGDLLLQAVQQSCLADHLLTASWGADPVPTKAPGEGQEGLPLTGACAGGPGLPAGELYGWAVETTPSPGPQPAALTTGEAAAPESPHQAEPYLSPSPSACTAVQEPSPGALDVTIMYKGRTVLQKVVGHPSCTFLYGPPDPAVRATDPQQVAFPSPAELPDQKQLRYTEELLRHVAPGLHLELRGPQLWARRMGKCKVYWEVGGPPGSASPSTPACLLPRNCDTPIFDFRVFFQELVEFRARQRRGSPRYTIYLGFGQDLSAGRPKEKSLVLVKLEPWLCRVHLEGTQREGVSSLDSSSLSLCLSSANSLYDDIECFLMELEQPA
PTMs - Q92985 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Ubiquitination | Uniprot | ||
| K50 | Ubiquitination | Uniprot | |
| S53 | Phosphorylation | Uniprot | |
| K61 | Ubiquitination | Uniprot | |
| K92 | Acetylation | Uniprot | |
| K92 | Ubiquitination | Uniprot | |
| K120 | Ubiquitination | Uniprot | |
| K303 | Ubiquitination | Uniprot | |
| K341 | Ubiquitination | Uniprot | |
| K375 | Ubiquitination | Uniprot | |
| T391 | Phosphorylation | Uniprot | |
| K444 | Ubiquitination | Uniprot | |
| K446 | Ubiquitination | Uniprot | |
| K452 | Ubiquitination | Uniprot | |
| S471 | Phosphorylation | Q9UHD2 (TBK1) , Q14164 (IKBKE) | Uniprot |
| S472 | Phosphorylation | Q9UHD2 (TBK1) , Q14164 (IKBKE) | Uniprot |
| S477 | Phosphorylation | Q14164 (IKBKE) , Q9UHD2 (TBK1) | Uniprot |
| S479 | Phosphorylation | Q14164 (IKBKE) , Q9UHD2 (TBK1) | Uniprot |
| S483 | Phosphorylation | Uniprot | |
| S487 | Phosphorylation | Uniprot |
研究背景
Key transcriptional regulator of type I interferon (IFN)-dependent immune responses and plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Can efficiently activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction via both the virus-activated, MyD88-independent pathway and the TLR-activated, MyD88-dependent pathway. Induces transcription of ubiquitin hydrolase USP25 mRNA in response to lipopolysaccharide (LPS) or viral infection in a type I IFN-dependent manner (By similarity). Required during both the early and late phases of the IFN gene induction but is more critical for the late than for the early phase. Exists in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, becomes phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization where along with other coactivators it can activate transcription of the type I IFN and ISG genes. Can also play a role in regulating adaptive immune responses by inducing PSMB9/LMP2 expression, either directly or through induction of IRF1. Binds to the Q promoter (Qp) of EBV nuclear antigen 1 a (EBNA1) and may play a role in the regulation of EBV latency. Can activate distinct gene expression programs in macrophages and regulate the anti-tumor properties of primary macrophages.
Acetylation inhibits its DNA-binding ability and activity.
In response to a viral infection, phosphorylated on Ser-477 and Ser-479 by TBK1 and IKBKE1. Phosphorylation, and subsequent activation is inhibited by vaccinia virus protein E3. In TLR7- and TLR9-mediated signaling pathway, phosphorylated by IRAK1.
TRAF6-mediated ubiquitination is required for IRF7 activation.
Sumoylated by TRIM28, which inhibits its transactivation activity.
(Microbial infection) Cleaved and inactivated by the protease 3C of enterovirus 71 allowing the virus to disrupt the host type I interferon production.
(Microbial infection) Cleaved and inactivated by the protease 3C of human enterovirus 68D (EV68) allowing the virus to disrupt the host type I interferon production.
Nucleus. Cytoplasm.
Note: The phosphorylated and active form accumulates selectively in the nucleus.
Expressed predominantly in spleen, thymus and peripheral blood leukocytes.
Monomer. Homodimer; phosphorylation-induced. Heterodimer with IRF3. Interacts with TICAM1 and TICAM2. Interacts with MYD88 AND TRAF6.
(Microbial infection) Interacts with Epstein-Barr virus LF2 and LMP1.
(Microbial infection) Interacts with rotavirus A NSP1; this interaction leads to the proteasome-dependent degradation of IRF7.
(Microbial infection) Interacts with human herpes virus 8/HHV-8 proteins ORF45 and vIRF-1.
(Microbial infection) Interacts with human T-cell leukemia virus 1/HTLV-1 protein HBZ.
(Microbial infection) Interacts with Seneca Valley virus protease 3C; this interaction is involved in the suppression of IRF7 expression and phosphorylation by the virus.
Belongs to the IRF family.
研究領域
· Human Diseases > Infectious diseases: Viral > Hepatitis C.
· Human Diseases > Infectious diseases: Viral > Hepatitis B.
· Human Diseases > Infectious diseases: Viral > Measles.
· Human Diseases > Infectious diseases: Viral > Influenza A.
· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
· Organismal Systems > Immune system > Toll-like receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway. (View pathway)
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