製品: Phospho-Transferrin Receptor (Tyr20) Antibody
カタログ: AF3896
タンパク質の説明: Rabbit polyclonal antibody to Phospho-Transferrin Receptor (Tyr20)
アプリケーション: IHC
反応性: Human, Mouse
分子量: 85kD(Calculated).
ユニプロット: P02786
RRID: AB_2847210

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製品説明

ソース:
Rabbit
アプリケーション:
IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
クローナリティ:
Polyclonal
特異性:
Phospho-Transferrin Receptor (Tyr20) Antibody detects endogenous levels of Transferrin Receptor only when phosphorylated at Tyr20.
RRID:
AB_2847210
引用形式: Affinity Biosciences Cat# AF3896, RRID:AB_2847210.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

CD 71; CD71; CD71 antigen; IMD46; OTTHUMP00000208523; OTTHUMP00000208524; OTTHUMP00000208525; p90; sTfR; T9; TFR 1; TfR; TfR1; TFR1_HUMAN; TFRC; TR; Transferrin receptor (p90 CD71); Transferrin receptor protein 1, serum form; Trfr;

免疫原

免疫原:

A synthesized peptide derived from human Transferrin Receptor around the phosphorylation site of Tyr20.

Uniprot:
遺伝子(ID):
タンパク質配列:
MMDQARSAFSNLFGGEPLSYTRFSLARQVDGDNSHVEMKLAVDEEENADNNTKANVTKPKRCSGSICYGTIAVIVFFLIGFMIGYLGYCKGVEPKTECERLAGTESPVREEPGEDFPAARRLYWDDLKRKLSEKLDSTDFTGTIKLLNENSYVPREAGSQKDENLALYVENQFREFKLSKVWRDQHFVKIQVKDSAQNSVIIVDKNGRLVYLVENPGGYVAYSKAATVTGKLVHANFGTKKDFEDLYTPVNGSIVIVRAGKITFAEKVANAESLNAIGVLIYMDQTKFPIVNAELSFFGHAHLGTGDPYTPGFPSFNHTQFPPSRSSGLPNIPVQTISRAAAEKLFGNMEGDCPSDWKTDSTCRMVTSESKNVKLTVSNVLKEIKILNIFGVIKGFVEPDHYVVVGAQRDAWGPGAAKSGVGTALLLKLAQMFSDMVLKDGFQPSRSIIFASWSAGDFGSVGATEWLEGYLSSLHLKAFTYINLDKAVLGTSNFKVSASPLLYTLIEKTMQNVKHPVTGQFLYQDSNWASKVEKLTLDNAAFPFLAYSGIPAVSFCFCEDTDYPYLGTTMDTYKELIERIPELNKVARAAAEVAGQFVIKLTHDVELNLDYERYNSQLLSFVRDLNQYRADIKEMGLSLQWLYSARGDFFRATSRLTTDFGNAEKTDRFVMKKLNDRVMRVEYHFLSPYVSPKESPFRHVFWGSGSHTLPALLENLKLRKQNNGAFNETLFRNQLALATWTIQGAANALSGDVWDIDNEF

PTMs - P02786 基板として

Site PTM Type Enzyme
S7 Phosphorylation
S10 Phosphorylation
S19 Phosphorylation
Y20 Phosphorylation P12931 (SRC)
T21 Phosphorylation
S24 Phosphorylation P17252 (PRKCA)
S34 Phosphorylation
K39 Ubiquitination
T52 Phosphorylation
K53 Acetylation
K53 Ubiquitination
K58 Ubiquitination
K60 Ubiquitination
T104 O-Glycosylation
S106 Phosphorylation
Y123 Phosphorylation
K128 Acetylation
K128 Ubiquitination
K134 Ubiquitination
K145 Ubiquitination
K161 Ubiquitination
Y168 Phosphorylation
K177 Ubiquitination
K189 Ubiquitination
K193 Ubiquitination
S195 Phosphorylation
S199 Phosphorylation
K205 Ubiquitination
Y219 Phosphorylation
K224 Ubiquitination
K231 Ubiquitination
K240 Ubiquitination
K241 Ubiquitination
N251 N-Glycosylation
K261 Ubiquitination
Y282 Phosphorylation
T286 Phosphorylation
N317 N-Glycosylation
S326 Phosphorylation
S327 Phosphorylation
S338 Phosphorylation
K344 Ubiquitination
K358 Ubiquitination
K371 Ubiquitination
K374 Ubiquitination
K382 Ubiquitination
K385 Ubiquitination
K394 Acetylation
Y402 Phosphorylation
K418 Acetylation
K418 Ubiquitination
S419 Phosphorylation
Y481 Phosphorylation
K486 Ubiquitination
S499 Phosphorylation
Y503 Phosphorylation
K508 Ubiquitination
K514 Ubiquitination
Y523 Phosphorylation
K531 Ubiquitination
T572 Phosphorylation
Y573 Phosphorylation
K585 Ubiquitination
Y611 Phosphorylation
S616 Phosphorylation
S620 Phosphorylation
T658 Phosphorylation
K665 Ubiquitination
Y683 Phosphorylation
S687 Phosphorylation
S691 Phosphorylation
K693 Ubiquitination
K717 Ubiquitination
K720 Ubiquitination

研究背景

機能:

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake.

(Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.

PTMs:

N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.

Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).

Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.

細胞の位置付け:

Cell membrane>Single-pass type II membrane protein. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Homodimer; disulfide-linked. Binds one transferrin or HFE molecule per subunit. Binds the HLA class II histocompatibility antigen, DR1. Interacts with SH3BP3. Interacts with STEAP3; facilitates TFRC endocytosis in erythroid precursor cells.

(Microbial infection) Interacts with Guanarito, Junin and Machupo arenavirus glycoprotein complex.

タンパク質ファミリー:

Belongs to the peptidase M28 family. M28B subfamily.

研究領域

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

· Cellular Processes > Cell growth and death > Ferroptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Hematopoietic cell lineage.   (View pathway)

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