製品: Calsenilin/KCNIP3 Antibody
カタログ: AF6509
タンパク質の説明: Rabbit polyclonal antibody to Calsenilin/KCNIP3
アプリケーション: IF/ICC
反応性: Human, Mouse, Rat
分子量: 29kD(Calculated).
ユニプロット: Q9Y2W7
RRID: AB_2847233

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製品説明

ソース:
Rabbit
アプリケーション:
IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
Calsenilin/KCNIP3 Antibody detects endogenous levels of total Calsenilin/KCNIP3.
RRID:
AB_2847233
引用形式: Affinity Biosciences Cat# AF6509, RRID:AB_2847233.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

4933407H12Rik; A type potassium channel modulatory protein 3; A-type potassium channel modulatory protein 3; AI413860; Calsenilin; CSEN; CSEN_HUMAN; DRE antagonist modulator; DRE-antagonist modulator; DREAM; EF hand transcription factor; KCHIP 3; KChIP3; KCNIP 3; Kcnip3; Kv channel interacting protein 3; Kv channel-interacting protein 3; MGC18289; Potassium channel interacting protein 3; R74849;

免疫原

免疫原:

A synthesized peptide derived from human Calsenilin/KCNIP3.

Uniprot:
遺伝子(ID):
発現特異性:
Q9Y2W7 CSEN_HUMAN:

Highly expressed in brain. Widely expressed at lower levels. Expression levels are elevated in brain cortex regions affected by Alzheimer disease.

タンパク質配列:
MQPAKEVTKASDGSLLGDLGHTPLSKKEGIKWQRPRLSRQALMRCCLVKWILSSTAPQGSDSSDSELELSTVRHQPEGLDQLQAQTKFTKKELQSLYRGFKNECPTGLVDEDTFKLIYAQFFPQGDATTYAHFLFNAFDADGNGAIHFEDFVVGLSILLRGTVHEKLKWAFNLYDINKDGYITKEEMLAIMKSIYDMMGRHTYPILREDAPAEHVERFFEKMDRNQDGVVTIEEFLEACQKDENIMSSMQLFENVI

PTMs - Q9Y2W7 基板として

Site PTM Type Enzyme
T8 Phosphorylation
S14 Phosphorylation
K26 Sumoylation
S63 Phosphorylation P48729 (CSNK1A1)
K90 Sumoylation
S95 Phosphorylation P25098 (GRK2)
S156 Phosphorylation
Y174 Phosphorylation

研究背景

機能:

Calcium-dependent transcriptional repressor that binds to the DRE element of genes including PDYN and FOS. Affinity for DNA is reduced upon binding to calcium and enhanced by binding to magnesium. Seems to be involved in nociception (By similarity).

Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels, such as KCND2/Kv4.2 and KCND3/Kv4.3. Modulates channel expression at the cell membrane, gating characteristics, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner.

May play a role in the regulation of PSEN2 proteolytic processing and apoptosis. Together with PSEN2 involved in modulation of amyloid-beta formation.

PTMs:

Palmitoylated. Palmitoylation enhances association with the plasma membrane (By similarity).

Proteolytically cleaved by caspase-3.

Phosphorylation at Ser-63 inhibits cleavage by CASP3.

細胞の位置付け:

Cytoplasm. Cell membrane>Lipid-anchor. Endoplasmic reticulum. Golgi apparatus. Nucleus.
Note: Also membrane-bound, associated with the plasma membrane (PubMed:15485870). In the presence of PSEN2 associated with the endoplasmic reticulum and Golgi. The sumoylated form is present only in the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Highly expressed in brain. Widely expressed at lower levels. Expression levels are elevated in brain cortex regions affected by Alzheimer disease.

サブユニット構造:

Binds to DNA as a homomultimer. Dimerization is induced by binding to calcium. Interacts with the C-terminus of PSEN1 and PSEN2 and with PSEN2 CTF subunit. Associates with KCN1. Component of heteromultimeric potassium channels. Identified in potassium channel complexes containing KCND1, KCND2, KCND3, KCNIP1, KCNIP2, KCNIP3, KCNIP4, DPP6 and DPP10 (By similarity). Interacts with KCND2 and KCND3.

タンパク質ファミリー:

Belongs to the recoverin family.

Restrictive clause

 

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