Nanog Antibody - #AF6535

製品: Nanog Antibody
カタログ: AF6535
タンパク質の説明: Rabbit polyclonal antibody to Nanog
アプリケーション: ELISA(peptide)
反応性: Human, Mouse, Rat
分子量: 35kD(Calculated).
ユニプロット: Q9H9S0
RRID: AB_2847259

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IHC Protocol
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製品説明

ソース:
Rabbit
アプリケーション:
ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
Nanog Antibody detects endogenous levels of total Nanog.
RRID:
AB_2847259
引用形式: Affinity Biosciences Cat# AF6535, RRID:AB_2847259.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Embryonic stem cell specific homeobox protein (Nanog); ENK; FLJ12581; hNanog; Homeobox protein NANOG; Homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; NANOG; Nanog homeobox; NANOG_HUMAN;

免疫原

免疫原:

A synthesized peptide derived from human Nanog.

Uniprot:

Q9H9S0(NANOG_HUMAN) >>Visit HPA database.

遺伝子(ID):
NANOG(79923)
発現特異性:
Q9H9S0 NANOG_HUMAN:

Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.

タンパク質配列:
MSVDPACPQSLPCFEASDCKESSPMPVICGPEENYPSLQMSSAEMPHTETVSPLPSSMDLLIQDSPDSSTSPKGKQPTSAEKSVAKKEDKVPVKKQKTRTVFSSTQLCVLNDRFQRQKYLSLQQMQELSNILNLSYKQVKTWFQNQRMKSKRWQKNNWPKNSNGVTQKASAPTYPSLYSSYHQGCLVNPTGNLPMWSNQTWNNSTWSNQTQNIQSWSNHSWNTQTWCTQSWNNQAWNSPFYNCGEESLQSCMQFQPNSPASDLEAALEAAGEGLNVIQQTTRYFSTPQTMDLFLNYSMNMQPEDV

PTMs - Q9H9S0 基板として

Site PTM Type Enzyme
S2 Phosphorylation
Y35 Phosphorylation Q05397 (PTK2)
T50 Phosphorylation
S52 Phosphorylation P28482 (MAPK1)
S56 Phosphorylation
S57 Phosphorylation
S65 Phosphorylation
T70 Phosphorylation
S71 Phosphorylation P28482 (MAPK1) , P06493 (CDK1)
T78 Phosphorylation Q02156 (PRKCE) , P28482 (MAPK1)
S79 Phosphorylation Q02156 (PRKCE)
S135 Phosphorylation Q02156 (PRKCE)
Y174 Phosphorylation Q05397 (PTK2)
T200 Phosphorylation Q02156 (PRKCE)
S258 Phosphorylation
T280 Phosphorylation Q02156 (PRKCE)
T286 Phosphorylation
T289 Phosphorylation

研究背景

機能:

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.

サブユニット構造:

Interacts with SMAD1 and SALL4. Interacts with ZNF281/ZFP281 (By similarity). Interacts with PCGF1. Interacts with ESRRB; reciprocally modulates their transcriptional activities (By similarity).

タンパク質ファミリー:

Belongs to the Nanog homeobox family.

研究領域

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

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