c-Myb Antibody - #AF6136
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製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF6136, RRID:AB_2835019.
折りたたみ／展開
C myb; C myb protein; Cmyb; MYB; Myb proto oncogene; V myb myeloblastosis viral oncogene homolog;
免疫原
- P10242 MYB_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MARRPRHSIYSSDEDDEDFEMCDHDYDGLLPKSGKRHLGKTRWTREEDEKLKKLVEQNGTDDWKVIANYLPNRTDVQCQHRWQKVLNPELIKGPWTKEEDQRVIELVQKYGPKRWSVIAKHLKGRIGKQCRERWHNHLNPEVKKTSWTEEEDRIIYQAHKRLGNRWAEIAKLLPGRTDNAIKNHWNSTMRRKVEQEGYLQESSKASQPAVATSFQKNSHLMGFAQAPPTAQLPATGQPTVNNDYSYYHISEAQNVSSHVPYPVALHVNIVNVPQPAAAAIQRHYNDEDPEKEKRIKELELLLMSTENELKGQQVLPTQNHTCSYPGWHSTTIADHTRPHGDSAPVSCLGEHHSTPSLPADPGSLPEESASPARCMIVHQGTILDNVKNLLEFAETLQFIDSFLNTSSNHENSDLEMPSLTSTPLIGHKLTVTTPFHRDQTVKTQKENTVFRTPAIKRSILESSPRTPTPFKHALAAQEIKYGPLKMLPQTPSHLVEDLQDVIKQESDESGIVAEFQENGPPLLKKIKQEVESPTDKSGNFFCSHHWEGDSLNTQLFTQTSPVADAPNILTSSVLMAPASEDEDNVLKAFTVPKNRSLASPLQPCSSTWEPASCGKMEEQMTSSSQARKYVNAFSARTLVM
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P10242 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S11 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S12 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| K53 | Ubiquitination | Uniprot | |
| Y69 | Phosphorylation | Uniprot | |
| K84 | Ubiquitination | Uniprot | |
| K92 | Ubiquitination | Uniprot | |
| K97 | Ubiquitination | Uniprot | |
| K109 | Ubiquitination | Uniprot | |
| K113 | Ubiquitination | Uniprot | |
| S116 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| K120 | Ubiquitination | Uniprot | |
| C130 | S-Nitrosylation | Uniprot | |
| K143 | Sumoylation | Uniprot | |
| K143 | Ubiquitination | Uniprot | |
| K160 | Ubiquitination | Uniprot | |
| K171 | Ubiquitination | Uniprot | |
| K182 | Ubiquitination | Uniprot | |
| K192 | Ubiquitination | Uniprot | |
| K204 | Ubiquitination | Uniprot | |
| K291 | Ubiquitination | Uniprot | |
| K293 | Ubiquitination | Uniprot | |
| K296 | Ubiquitination | Uniprot | |
| T420 | Phosphorylation | Uniprot | |
| T433 | Phosphorylation | Uniprot | |
| K442 | Ubiquitination | Uniprot | |
| K445 | Acetylation | Uniprot | |
| K445 | Ubiquitination | Uniprot | |
| T452 | Phosphorylation | Uniprot | |
| R457 | Methylation | Uniprot | |
| S463 | Phosphorylation | Uniprot | |
| R465 | Methylation | Uniprot | |
| T466 | Phosphorylation | Uniprot | |
| T468 | Phosphorylation | Uniprot | |
| K471 | Acetylation | Uniprot | |
| K471 | Methylation | Uniprot | |
| K471 | Ubiquitination | Uniprot | |
| K480 | Acetylation | Uniprot | |
| K480 | Sumoylation | Uniprot | |
| K480 | Ubiquitination | Uniprot | |
| K485 | Acetylation | Uniprot | |
| K485 | Ubiquitination | Uniprot | |
| K503 | Sumoylation | Uniprot | |
| K527 | Sumoylation | Uniprot | |
| S532 | Phosphorylation | P27361 (MAPK3) | Uniprot |
| T534 | Phosphorylation | Uniprot | |
| K593 | Ubiquitination | Uniprot | |
| S599 | Phosphorylation | Uniprot | |
| K615 | Ubiquitination | Uniprot | |
| T621 | Phosphorylation | Uniprot | |
| S624 | Phosphorylation | Uniprot | |
| K628 | Ubiquitination | Uniprot |
研究背景
Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.
Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
Phosphorylated by NLK on multiple sites, which induces proteasomal degradation.
Phosphorylated by HIPK1. This phosphorylation reduces MYB transcription factor activity but not MYB protein levels.
Nucleus.
Binds MYBBP1A. Interacts with HIPK2, MAF and NLK (By similarity). Binds to HIPK1.
Comprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C-terminal domain involved in transcriptional repression.
研究領域
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
参考文献
Application: WB Species: human Sample: Gastric cancer cells
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