CRTC3 Antibody - #AF6576
| 製品: | CRTC3 Antibody |
| カタログ: | AF6576 |
| タンパク質の説明: | Rabbit polyclonal antibody to CRTC3 |
| アプリケーション: | WB |
| 反応性: | Human, Mouse, Rat |
| 分子量: | 67kD(Calculated). |
| ユニプロット: | Q6UUV7 |
| RRID: | AB_2847300 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF6576, RRID:AB_2847300.
折りたたみ／展開
CREB regulated transcription coactivator 3; CREB-regulated transcription coactivator 3; CRTC 3; CRTC3; CRTC3_HUMAN; FLJ21868; TORC 3; TORC-3; TORC3; Transducer of CREB protein 3; Transducer of regulated cAMP response element binding protein (CREB) 3; Transducer of regulated cAMP response element binding protein 3; Transducer of regulated cAMP response element-binding protein 3; Transducer of regulated CREB protein 3;
免疫原
Predominantly expressed in B and T lymphocytes. Highest levels in lung. Also expressed in brain, colon, heart, kidney, ovary, and prostate. Weak expression in liver, pancreas, muscle, small intestine, spleen and stomach.
- Q6UUV7 CRTC3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAASPGSGSANPRKFSEKIALHTQRQAEETRAFEQLMTDLTLSRVQFQKLQQLRLTQYHGGSLPNVSQLRSSASEFQPSFHQADNVRGTRHHGLVERPSRNRFHPLHRRSGDKPGRQFDGSAFGANYSSQPLDESWPRQQPPWKDEKHPGFRLTSALNRTNSDSALHTSALSTKPQDPYGGGGQSAWPAPYMGFCDGENNGHGEVASFPGPLKEENLLNVPKPLPKQLWETKEIQSLSGRPRSCDVGGGNAFPHNGQNLGLSPFLGTLNTGGSLPDLTNLHYSTPLPASLDTTDHHFGSMSVGNSVNNIPAAMTHLGIRSSSGLQSSRSNPSIQATLNKTVLSSSLNNHPQTSVPNASALHPSLRLFSLSNPSLSTTNLSGPSRRRQPPVSPLTLSPGPEAHQGFSRQLSSTSPLAPYPTSQMVSSDRSQLSFLPTEAQAQVSPPPPYPAPQELTQPLLQQPRAPEAPAQQPQAASSLPQSDFQLLPAQGSSLTNFFPDVGFDQQSMRPGPAFPQQVPLVQQGSRELQDSFHLRPSPYSNCGSLPNTILPEDSSTSLFKDLNSALAGLPEVSLNVDTPFPLEEELQIEPLSLDGLNMLSDSSMGLLDPSVEETFRADRL
PTMs - Q6UUV7 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S4 | Phosphorylation | Uniprot | |
| K18 | Sumoylation | Uniprot | |
| K18 | Ubiquitination | Uniprot | |
| T56 | Phosphorylation | Uniprot | |
| Y58 | Phosphorylation | Uniprot | |
| S62 | Phosphorylation | P57059 (SIK1) | Uniprot |
| S67 | Phosphorylation | Uniprot | |
| R87 | Methylation | Uniprot | |
| S99 | Phosphorylation | Uniprot | |
| S135 | Phosphorylation | Uniprot | |
| K147 | Sumoylation | Uniprot | |
| T160 | Phosphorylation | Uniprot | |
| S162 | Phosphorylation | P57059 (SIK1) | Uniprot |
| K174 | Sumoylation | Uniprot | |
| K213 | Sumoylation | Uniprot | |
| K222 | Sumoylation | Uniprot | |
| K226 | Sumoylation | Uniprot | |
| K232 | Sumoylation | Uniprot | |
| K232 | Ubiquitination | Uniprot | |
| S238 | Phosphorylation | Uniprot | |
| S273 | Phosphorylation | Uniprot | |
| S305 | Phosphorylation | Uniprot | |
| S321 | Phosphorylation | Uniprot | |
| S327 | Phosphorylation | Uniprot | |
| S329 | Phosphorylation | P57059 (SIK1) | Uniprot |
| S332 | Phosphorylation | Uniprot | |
| S344 | Phosphorylation | Uniprot | |
| S345 | Phosphorylation | Uniprot | |
| T352 | Phosphorylation | Uniprot | |
| S353 | Phosphorylation | Uniprot | |
| S363 | Phosphorylation | Uniprot | |
| S368 | Phosphorylation | Uniprot | |
| S370 | Phosphorylation | P57059 (SIK1) | Uniprot |
| S373 | Phosphorylation | Uniprot | |
| S375 | Phosphorylation | Uniprot | |
| S380 | Phosphorylation | Uniprot | |
| S391 | Phosphorylation | Uniprot | |
| T394 | Phosphorylation | Uniprot | |
| S396 | Phosphorylation | Uniprot | |
| S406 | Phosphorylation | Uniprot | |
| S410 | Phosphorylation | Uniprot | |
| S411 | Phosphorylation | Uniprot | |
| T412 | Phosphorylation | Uniprot | |
| S413 | Phosphorylation | Uniprot | |
| S432 | Phosphorylation | Uniprot | |
| T436 | Phosphorylation | Uniprot | |
| S443 | Phosphorylation | Uniprot | |
| Y448 | Phosphorylation | Uniprot | |
| T455 | Phosphorylation | Uniprot | |
| S524 | Phosphorylation | Uniprot | |
| T577 | Phosphorylation | Uniprot |
研究背景
Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR).
Phosphorylation/dephosphorylation states of Ser-273 are required for regulating transduction of CREB activity (By similarity). CRTCs/TORCs are inactive when phosphorylated, and active when dephosphorylated at this site (By similarity). May be phosphorylated at Ser-391 by MAPK3/ERK1 and/or MAPK1/ERK2 or by some cyclin-dependent kinases such as CDK1,CDK2 or CDK5. Following adenylyl cyclase activation, dephosphorylated at Ser-162 and Ser-273 resulting in its dissociation from 14-3-3 proteins probably promoting CRTC3 translocation into the nucleus.
Nucleus. Cytoplasm.
Note: Appears to be mainly nuclear (PubMed:15454081). Translocates to the nucleus following adenylyl cyclase or MAP kinase activation (PubMed:30611118).
Predominantly expressed in B and T lymphocytes. Highest levels in lung. Also expressed in brain, colon, heart, kidney, ovary, and prostate. Weak expression in liver, pancreas, muscle, small intestine, spleen and stomach.
Binding, as a tetramer, through its N-terminal region, with the bZIP domain of CREB1 enhances recruitment of TAF4 to the promoter. 'Arg-314' in the bZIP domain of CREB1 is essential for this interaction. Interacts (when phosphorylated at Ser-162 and Se-273) with 14-3-3 proteins. Interacts with YWHAE. Interacts (when phosphorylated at Ser-391) with phosphatase PP2A catalytic subunit PPP2CA and regulatory subunits PPP2R1A and PPP2R2A. Interacts, via the N-terminal with the ankyrin repeats of BCL3, to form a complex with CREB1 on CRE and TxRE responsive elements and represses HTLV-1 LTR-mediated transcription.
(Microbial infection) Interacts with HTLV-1 protein Tax; this interaction enhances tax transcriptional activity.
Belongs to the TORC family.
研究領域
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.