MAP3K8/COT Antibody - #AF6670
製品: | MAP3K8/COT Antibody |
カタログ: | AF6670 |
タンパク質の説明: | Rabbit polyclonal antibody to MAP3K8/COT |
アプリケーション: | WB |
反応性: | Human, Mouse, Rat |
分子量: | 53kD(Calculated). |
ユニプロット: | P41279 |
RRID: | AB_2847393 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF6670, RRID:AB_2847393.
折りたたみ/展開
AURA2; c COT; Cancer Osaka thyroid oncogene; CCOT; COT; COT proto oncogene serine/threonine protein kinase; EST; ESTF; Ewing sarcoma transformant; FLJ10486; M3K8_HUMAN; MAP3K 8; MAP3K8; MEKK8; Mitogen activated protein kinase kinase kinase 8; Mitogen-activated protein kinase kinase kinase 8; Proto oncogene cCot; Proto-oncogene c-Cot; Serine/threonine protein kinase cot; Serine/threonine-protein kinase cot; TPL 2; TPL-2; TPL2; Tumor progression locus 2;
免疫原
A synthesized peptide derived from human MAP3K8/COT.
- P41279 M3K8_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEYMSTGSDNKEEIDLLIKHLNVSDVIDIMENLYASEEPAVYEPSLMTMCQDSNQNDERSKSLLLSGQEVPWLSSVRYGTVEDLLAFANHISNTAKHFYGQRPQESGILLNMVITPQNGRYQIDSDVLLIPWKLTYRNIGSDFIPRGAFGKVYLAQDIKTKKRMACKLIPVDQFKPSDVEIQACFRHENIAELYGAVLWGETVHLFMEAGEGGSVLEKLESCGPMREFEIIWVTKHVLKGLDFLHSKKVIHHDIKPSNIVFMSTKAVLVDFGLSVQMTEDVYFPKDLRGTEIYMSPEVILCRGHSTKADIYSLGATLIHMQTGTPPWVKRYPRSAYPSYLYIIHKQAPPLEDIADDCSPGMRELIEASLERNPNHRPRAADLLKHEALNPPREDQPRCQSLDSALLERKRLLSRKELELPENIADSSCTGSTEESEMLKRQRSLYIDLGALAGYFNLVRGPPTLEYG
PTMs - P41279 基板として
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S62 | Phosphorylation | Uniprot | |
T80 | Phosphorylation | Uniprot | |
S125 | Phosphorylation | Uniprot | |
Y136 | Phosphorylation | Uniprot | |
S141 | Phosphorylation | Uniprot | |
Y153 | Phosphorylation | Uniprot | |
T290 | Phosphorylation | Uniprot | |
S334 | Phosphorylation | Uniprot | |
S368 | Phosphorylation | Uniprot | |
S400 | Phosphorylation | P31749 (AKT1) , Q9P1W9 (PIM2) | Uniprot |
S413 | Phosphorylation | Q9P1W9 (PIM2) , P31749 (AKT1) | Uniprot |
S443 | Phosphorylation | P41279 (MAP3K8) | Uniprot |
PTMs - P41279 酵素として
Substrate | Site | Source |
---|---|---|
O14757 (CHEK1) | S345 | Uniprot |
P00519 (ABL1) | T735 | Uniprot |
P06493 (CDK1) | T161 | Uniprot |
P06748 (NPM1) | T199 | Uniprot |
P15311 (EZR) | T567 | Uniprot |
P16220 (CREB1) | S133 | Uniprot |
P26038 (MSN) | T558 | Uniprot |
P35241 (RDX) | T564 | Uniprot |
P36507 (MAP2K2) | S222 | Uniprot |
P36507 (MAP2K2) | S226 | Uniprot |
P41279 (MAP3K8) | S443 | Uniprot |
P46734 (MAP2K3) | S218 | Uniprot |
P52564 (MAP2K6) | S207 | Uniprot |
P53350 (PLK1) | S137 | Uniprot |
P53350 (PLK1) | T210 | Uniprot |
Q02750 (MAP2K1) | S218 | Uniprot |
Q02750 (MAP2K1) | S222 | Uniprot |
Q02750 (MAP2K1) | S244 | Uniprot |
Q02750 (MAP2K1) | S248 | Uniprot |
Q04637 (EIF4G1) | S1147 | Uniprot |
Q13526 (PIN1) | S16 | Uniprot |
Q13541 (EIF4EBP1) | T70 | Uniprot |
Q15418 (RPS6KA1) | S380 | Uniprot |
Q99558 (MAP3K14) | T559 | Uniprot |
研究背景
Required for lipopolysaccharide (LPS)-induced, TLR4-mediated activation of the MAPK/ERK pathway in macrophages, thus being critical for production of the proinflammatory cytokine TNF-alpha (TNF) during immune responses. Involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells. Involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production. In vitro, activates MAPK/ERK pathway in response to IL1 in an IRAK1-independent manner, leading to up-regulation of IL8 and CCL4. Transduces CD40 and TNFRSF1A signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. May also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types. In adipocytes, activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF, but not insulin, leading to induction of lipolysis. Plays a role in the cell cycle. Isoform 1 shows some transforming activity, although it is much weaker than that of the activated oncogenic variant.
Autophosphorylated. Isoform 1 undergoes phosphorylation mainly on Ser residues, and isoform 2 on both Ser and Thr residues. Phosphorylated on Thr-290; the phosphorylation is necessary but not sufficient for full kinase activity in vitro and for the dissociation of isoform 1 from NFKB1, leading to its degradation. Phosphorylated on Ser-400 by IKBKB; the phosphorylation is required for LPS-stimulated activation of the MAPK/ERK pathway in macrophages.
Cytoplasm.
Expressed in several normal tissues and human tumor-derived cell lines.
Forms a ternary complex with NFKB1/p105 and TNIP2. Interacts with NFKB1; the interaction increases the stability of MAP3K8 but inhibits its MEK phosphorylation activity, whereas loss of interaction following LPS stimulation leads to its degradation. Interacts with CD40 and TRAF6; the interaction is required for ERK activation. Interacts with KSR2; the interaction inhibits ERK and NF-kappa-B activation.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
研究領域
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)
· Organismal Systems > Immune system > Toll-like receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > T cell receptor signaling pathway. (View pathway)
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