eIF4B Antibody - #AF6700
(2)
| 製品: | eIF4B Antibody |
| カタログ: | AF6700 |
| タンパク質の説明: | Rabbit polyclonal antibody to eIF4B |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse |
| 分子量: | 70kD; 69kD(Calculated). |
| ユニプロット: | P23588 |
| RRID: | AB_2847423 |
製品説明
ソース:
Rabbit
アプリケーション:
IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
反応性:
Human,Mouse
クローナリティ:
Polyclonal
特異性:
eIF4B Antibody detects endogenous levels of total eIF4B.
RRID:
AB_2847423
引用形式: Affinity Biosciences Cat# AF6700, RRID:AB_2847423.
引用形式: Affinity Biosciences Cat# AF6700, RRID:AB_2847423.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:
折りたたみ／展開
2310046H11Rik; AL024095; C85189; EIF 4B; eIF-4B; EIF4B; Eukaryotic initiation factor 4B; Eukaryotic translation initiation factor 4B; IF4B_HUMAN; PRO1843;
免疫原
免疫原:
A synthesized peptide derived from human eIF4B.
Uniprot:
遺伝子(ID):
タンパク質配列:
- P23588 IF4B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAASAKKKNKKGKTISLTDFLAEDGGTGGGSTYVSKPVSWADETDDLEGDVSTTWHSNDDDVYRAPPIDRSILPTAPRAAREPNIDRSRLPKSPPYTAFLGNLPYDVTEESIKEFFRGLNISAVRLPREPSNPERLKGFGYAEFEDLDSLLSALSLNEESLGNRRIRVDVADQAQDKDRDDRSFGRDRNRDSDKTDTDWRARPATDSFDDYPPRRGDDSFGDKYRDRYDSDRYRDGYRDGYRDGPRRDMDRYGGRDRYDDRGSRDYDRGYDSRIGSGRRAFGSGYRRDDDYRGGGDRYEDRYDRRDDRSWSSRDDYSRDDYRRDDRGPPQRPKLNLKPRSTPKEDDSSASTSQSTRAASIFGGAKPVDTAAREREVEERLQKEQEKLQRQLDEPKLERRPRERHPSWRSEETQERERSRTGSESSQTGTSTTSSRNARRRESEKSLENETLNKEEDCHSPTSKPPKPDQPLKVMPAPPPKENAWVKRSSNPPARSQSSDTEQQSPTSGGGKVAPAQPSEEGPGRKDENKVDGMNAPKGQTGNSSRGPGDGGNRDHWKESDRKDGKKDQDSRSAPEPKKPEENPASKFSSASKYAALSVDGEDENEGEDYAE
PTMs - P23588 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K6 | Acetylation | Uniprot | |
| S16 | Phosphorylation | Uniprot | |
| Y33 | Phosphorylation | Uniprot | |
| S39 | Phosphorylation | Uniprot | |
| S52 | Phosphorylation | Uniprot | |
| S57 | Phosphorylation | Uniprot | |
| Y63 | Phosphorylation | Uniprot | |
| R70 | Methylation | Uniprot | |
| S71 | Phosphorylation | Uniprot | |
| T75 | Phosphorylation | Uniprot | |
| R78 | Methylation | Uniprot | |
| K92 | Ubiquitination | Uniprot | |
| S93 | Phosphorylation | Uniprot | |
| Y96 | Phosphorylation | Uniprot | |
| T97 | Phosphorylation | Uniprot | |
| Y105 | Phosphorylation | Uniprot | |
| S111 | Phosphorylation | Uniprot | |
| R117 | Methylation | Uniprot | |
| S131 | Phosphorylation | Uniprot | |
| Y141 | Phosphorylation | Uniprot | |
| S155 | Phosphorylation | Uniprot | |
| S160 | Phosphorylation | Uniprot | |
| K177 | Ubiquitination | Uniprot | |
| R182 | Methylation | Uniprot | |
| S183 | Phosphorylation | Uniprot | |
| S192 | Phosphorylation | Uniprot | |
| T195 | Phosphorylation | Uniprot | |
| T197 | Phosphorylation | Uniprot | |
| T205 | Phosphorylation | Uniprot | |
| S207 | Phosphorylation | Uniprot | |
| Y211 | Phosphorylation | Uniprot | |
| S219 | Phosphorylation | Uniprot | |
| K223 | Ubiquitination | Uniprot | |
| Y224 | Phosphorylation | Uniprot | |
| Y228 | Phosphorylation | Uniprot | |
| S230 | Phosphorylation | Uniprot | |
| Y233 | Phosphorylation | Uniprot | |
| Y237 | Phosphorylation | Uniprot | |
| Y241 | Phosphorylation | Uniprot | |
| Y252 | Phosphorylation | Uniprot | |
| Y258 | Phosphorylation | Uniprot | |
| R261 | Methylation | Uniprot | |
| S263 | Phosphorylation | Uniprot | |
| Y266 | Phosphorylation | Uniprot | |
| R268 | Methylation | Uniprot | |
| Y270 | Phosphorylation | Uniprot | |
| S272 | Phosphorylation | Uniprot | |
| R273 | Methylation | Uniprot | |
| R279 | Methylation | Uniprot | |
| S283 | Phosphorylation | Uniprot | |
| Y285 | Phosphorylation | Uniprot | |
| Y291 | Phosphorylation | Uniprot | |
| R292 | Methylation | Uniprot | |
| R297 | Methylation | Uniprot | |
| Y298 | Phosphorylation | Uniprot | |
| Y302 | Phosphorylation | Uniprot | |
| R308 | Methylation | Uniprot | |
| S309 | Phosphorylation | Uniprot | |
| S311 | Phosphorylation | Uniprot | |
| S312 | Phosphorylation | Uniprot | |
| Y316 | Phosphorylation | Uniprot | |
| S317 | Phosphorylation | Uniprot | |
| Y321 | Phosphorylation | Uniprot | |
| K333 | Sumoylation | Uniprot | |
| S340 | Phosphorylation | Uniprot | |
| T341 | Phosphorylation | Uniprot | |
| S347 | Phosphorylation | Uniprot | |
| S348 | Phosphorylation | Uniprot | |
| S350 | Phosphorylation | Uniprot | |
| T351 | Phosphorylation | Uniprot | |
| S352 | Phosphorylation | Uniprot | |
| S354 | Phosphorylation | Uniprot | |
| S359 | Phosphorylation | Uniprot | |
| K365 | Acetylation | Uniprot | |
| K365 | Ubiquitination | Uniprot | |
| T369 | Phosphorylation | Uniprot | |
| S406 | Phosphorylation | Q9P1W9 (PIM2) , Q14680 (MELK) | Uniprot |
| S409 | Phosphorylation | Uniprot | |
| T412 | Phosphorylation | Uniprot | |
| S418 | Phosphorylation | Uniprot | |
| T420 | Phosphorylation | Uniprot | |
| S422 | Phosphorylation | P51812 (RPS6KA3) , Q15418 (RPS6KA1) , P23443 (RPS6KB1) , P31749 (AKT1) | Uniprot |
| S424 | Phosphorylation | Uniprot | |
| S425 | Phosphorylation | Uniprot | |
| T427 | Phosphorylation | Uniprot | |
| T429 | Phosphorylation | Uniprot | |
| S430 | Phosphorylation | Uniprot | |
| T431 | Phosphorylation | Uniprot | |
| T432 | Phosphorylation | Uniprot | |
| S433 | Phosphorylation | Uniprot | |
| S434 | Phosphorylation | Uniprot | |
| S442 | Phosphorylation | Uniprot | |
| K444 | Acetylation | Uniprot | |
| S445 | Phosphorylation | Uniprot | |
| T450 | Phosphorylation | Uniprot | |
| S459 | Phosphorylation | Uniprot | |
| T461 | Phosphorylation | Uniprot | |
| S462 | Phosphorylation | Uniprot | |
| K463 | Ubiquitination | Uniprot | |
| K486 | Methylation | Uniprot | |
| S488 | Phosphorylation | Uniprot | |
| S489 | Phosphorylation | Uniprot | |
| S495 | Phosphorylation | Uniprot | |
| S497 | Phosphorylation | Uniprot | |
| S498 | Phosphorylation | Uniprot | |
| T500 | Phosphorylation | Uniprot | |
| S504 | Phosphorylation | Uniprot | |
| T506 | Phosphorylation | Uniprot | |
| S518 | Phosphorylation | Uniprot | |
| K537 | Ubiquitination | Uniprot | |
| S585 | Phosphorylation | Uniprot | |
| K586 | Acetylation | Uniprot | |
| K586 | Ubiquitination | Uniprot | |
| S589 | Phosphorylation | Uniprot | |
| S591 | Phosphorylation | Uniprot | |
| K592 | Ubiquitination | Uniprot | |
| Y593 | Phosphorylation | Uniprot | |
| S597 | Phosphorylation | Uniprot | |
| Y609 | Phosphorylation | Uniprot |
研究背景
機能:
Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
PTMs:
Phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex.
サブユニット構造:
Self-associates and interacts with EIF3 p170 subunit.
研究領域
· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Genetic Information Processing > Translation > RNA transport.
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.