Gli2 Antibody - #AF6733
| 製品: | Gli2 Antibody |
| カタログ: | AF6733 |
| タンパク質の説明: | Rabbit polyclonal antibody to Gli2 |
| アプリケーション: | ELISA(peptide) |
| 反応性: | Human, Mouse |
| 分子量: | 132kD,168kD,240kD; 168kD(Calculated). |
| ユニプロット: | P10070 |
| RRID: | AB_2847456 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF6733, RRID:AB_2847456.
折りたたみ／展開
CJS; Gli 2; GLI family zinc finger 2; GLI Kruppel family member GLI2; GLI2; GLI2_HUMAN; Glioma associated oncogene family zinc finger; HPE9; Oncogene GLI2; PHS2; Tax helper protein 1; Tax helper protein 2; Tax helper protein; Tax responsive element 2 holding protein; Tax responsive element 25 bp sequence binding protein; THP; THP1; THP2; Zinc finger protein GLI2;
免疫原
A synthesized peptide derived from human Gli2.
Expressed in breast cancers (at protein level) (PubMed:26565916). Isoform 1 and isoform 4 are expressed in HTLV-1-infected T-cell lines (at protein level) (PubMed:9557682). Isoform 1 and isoform 2 are strongly expressed in HTLV-1-infected T-cell lines (PubMed:9557682). Isoform 3 and isoform 4 are weakly expressed in HTLV-1-infected T-cell lines (PubMed:9557682).
- P10070 GLI2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
METSASATASEKQEAKSGILEAAGFPDPGKKASPLVVAAAAAAAVAAQGVPQHLLPPFHAPLPIDMRHQEGRYHYEPHSVHGVHGPPALSGSPVISDISLIRLSPHPAGPGESPFNAPHPYVNPHMEHYLRSVHSSPTLSMISAARGLSPADVAQEHLKERGLFGLPAPGTTPSDYYHQMTLVAGHPAPYGDLLMQSGGAASAPHLHDYLNPVDVSRFSSPRVTPRLSRKRALSISPLSDASLDLQRMIRTSPNSLVAYINNSRSSSAASGSYGHLSAGALSPAFTFPHPINPVAYQQILSQQRGLGSAFGHTPPLIQPSPTFLAQQPMALTSINATPTQLSSSSNCLSDTNQNKQSSESAVSSTVNPVAIHKRSKVKTEPEGLRPASPLALTQGQVSGHGSCGCALPLSQEQLADLKEDLDRDDCKQEAEVVIYETNCHWEDCTKEYDTQEQLVHHINNEHIHGEKKEFVCRWQACTREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYICKIPGCTKRYTDPSSLRKHVKTVHGPDAHVTKKQRNDVHLRTPLLKENGDSEAGTEPGGPESTEASSTSQAVEDCLHVRAIKTESSGLCQSSPGAQSSCSSEPSPLGSAPNNDSGVEMPGTGPGSLGDLTALDDTPPGADTSALAAPSAGGLQLRKHMTTMHRFEQLKKEKLKSLKDSCSWAGPTPHTRNTKLPPLPGSGSILENFSGSGGGGPAGLLPNPRLSELSASEVTMLSQLQERRDSSTSTVSSAYTVSRRSSGISPYFSSRRSSEASPLGAGRPHNASSADSYDPISTDASRRSSEASQCSGGSGLLNLTPAQQYSLRAKYAAATGGPPPTPLPGLERMSLRTRLALLDAPERTLPAGCPRPLGPRRGSDGPTYGHGHAGAAPAFPHEAPGGGARRASDPVRRPDALSLPRVQRFHSTHNVNPGPLPPCADRRGLRLQSHPSTDGGLARGAYSPRPPSISENVAMEAVAAGVDGAGPEADLGLPEDDLVLPDDVVQYIKAHASGALDEGTGQVYPTESTGFSDNPRLPSPGLHGQRRMVAADSNVGPSAPMLGGCQLGFGAPSSLNKNNMPVQWNEVSSGTVDALASQVKPPPFPQGNLAVVQQKPAFGQYPGYSPQGLQASPGGLDSTQPHLQPRSGAPSQGIPRVNYMQQLRQPVAGSQCPGMTTTMSPHACYGQVHPQLSPSTISGALNQFPQSCSNMPAKPGHLGHPQQTEVAPDPTTMGNRHRELGVPDSALAGVPPPHPVQSYPQQSHHLAASMSQEGYHQVPSLLPARQPGFMEPQTGPMGVATAGFGLVQPRPPLEPSPTGRHRGVRAVQQQLAYARATGHAMAAMPSSQETAEAVPKGAMGNMGSVPPQPPPQDAGGAPDHSMLYYYGQIHMYEQDGGLENLGSCQVMRSQPPQPQACQDSIQPQPLPSPGVNQVSSTVDSQLLEAPQIDFDAIMDDGDHSSLFSGALSPSLLHSLSQNSSRLTTPRNSLTLPSIPAGISNMAVGDMSSMLTSLAEESKFLNMMT
PTMs - P10070 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T8 | Phosphorylation | Uniprot | |
| S10 | Phosphorylation | Uniprot | |
| R131 | Methylation | Uniprot | |
| S132 | Phosphorylation | Uniprot | |
| S135 | Phosphorylation | Uniprot | |
| S136 | Phosphorylation | Uniprot | |
| S143 | Phosphorylation | Uniprot | |
| R146 | Methylation | Uniprot | |
| S149 | Phosphorylation | Uniprot | |
| T224 | Phosphorylation | Uniprot | |
| S234 | Phosphorylation | Uniprot | |
| S236 | Phosphorylation | Uniprot | |
| S239 | Phosphorylation | Uniprot | |
| S242 | Phosphorylation | Uniprot | |
| S252 | Phosphorylation | Uniprot | |
| S255 | Phosphorylation | Uniprot | |
| S388 | Phosphorylation | Q92630 (DYRK2) | Uniprot |
| K521 | Ubiquitination | Uniprot | |
| K757 | Acetylation | Uniprot | |
| S808 | Phosphorylation | Uniprot | |
| T818 | Phosphorylation | Uniprot | |
| S820 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| S824 | Phosphorylation | Uniprot | |
| S831 | Phosphorylation | Uniprot | |
| S832 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| S836 | Phosphorylation | Uniprot | |
| S839 | Phosphorylation | Uniprot | |
| S859 | Phosphorylation | Uniprot | |
| T860 | Phosphorylation | Uniprot | |
| S863 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| S870 | Phosphorylation | Uniprot | |
| T903 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| R910 | Methylation | Uniprot | |
| R916 | Methylation | Uniprot | |
| R939 | Methylation | Uniprot | |
| S941 | Phosphorylation | Uniprot | |
| S970 | Phosphorylation | Uniprot | |
| S989 | Phosphorylation | Uniprot | |
| T990 | Phosphorylation | Uniprot | |
| R1008 | Methylation | Uniprot | |
| S1011 | Phosphorylation | Q92630 (DYRK2) | Uniprot |
| S1014 | Phosphorylation | Uniprot | |
| R1021 | Methylation | Uniprot | |
| S1030 | Phosphorylation | Uniprot | |
| R1098 | Methylation | Uniprot | |
| S1101 | Phosphorylation | Uniprot | |
| S1378 | Phosphorylation | Uniprot | |
| R1387 | Methylation | Uniprot | |
| R1397 | Methylation | Uniprot |
研究背景
Functions as transcription regulator in the hedgehog (Hh) pathway. Functions as transcriptional activator. May also function as transcriptional repressor (By similarity). Requires STK36 for full transcriptional activator activity. Required for normal embryonic development.
Isoform 1, isoform 2, isoform 3 and isoform 4: Act as transcriptional activators in T-cell leukemia virus type 1 (HTLV-1)-infected cells in a Tax-dependent manner. Bind to the DNA sequence 5'-GAACCACCCA-3' which is part of the Tax-responsive element (TRE-2S) regulatory element that augments the Tax-dependent enhancer of HTLV-1. Are involved in the smoothened (SHH) signaling pathway.
Acts as a transcriptional repressor.
Phosphorylated in vitro by ULK3. Phosphorylated by DYRK2; this inhibits GLI2 transcription factor activity and promotes proteasomal degradation of GLI2.
Acetylation at Lys-757 inhibits Hh target gene expression, probably by impeding entry into chromatin thus preventing promoter occupancy.
Nucleus. Cytoplasm. Cell projection>Cilium.
Note: STK36 promotes translocation to the nucleus. In keratinocytes, it is sequestered in the cytoplasm by SUFU. In the absence of SUFU, it translocates to the nucleus.
Nucleus.
Nucleus.
Expressed in breast cancers (at protein level). Isoform 1 and isoform 4 are expressed in HTLV-1-infected T-cell lines (at protein level). Isoform 1 and isoform 2 are strongly expressed in HTLV-1-infected T-cell lines. Isoform 3 and isoform 4 are weakly expressed in HTLV-1-infected T-cell lines.
Interaction with ZIC1 and ZIC2 (By similarity). Interacts with STK36. Interacts with SUFU; this inhibits transcriptional activation mediated by GLI2. Interacts (via C-terminal internal region) with FOXC1 (via N-terminus); this interaction is direct and increases GLI2 DNA-binding and transcriptional activity through a smoothened (SMO)-independent Hedgehog (Hh) signaling pathway.
The N-terminal domain confers transcriptional repressor activity, while the C-terminal domain mediates transcriptional activation.
Belongs to the GLI C2H2-type zinc-finger protein family.
研究領域
· Environmental Information Processing > Signal transduction > Hedgehog signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Hippo signaling pathway. (View pathway)
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Basal cell carcinoma. (View pathway)
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