製品: Mitofusin 2 Antibody
カタログ: AF6760
タンパク質の説明: Rabbit polyclonal antibody to Mitofusin 2
アプリケーション: WB
反応性: Human, Mouse, Rat
分子量: 86kD; 86kD(Calculated).
ユニプロット: O95140
RRID: AB_2847483

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
Mitofusin 2 Antibody detects endogenous levels of total Mitofusin 2.
RRID:
AB_2847483
引用形式: Affinity Biosciences Cat# AF6760, RRID:AB_2847483.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

CMT2A; CMT2A2; CPRP 1; CPRP1; EC 3.6.5.-; Fzo; HSG; hyperplasia suppressor gene; Hypertension related protein 1; KIAA0214; MARF; MFN 2; Mfn2; MFN2_HUMAN; Mitochondrial assembly regulatory factor; Mitofusin-2; Mitofusin2; Transmembrane GTPase MFN2;

免疫原

免疫原:

A synthesized peptide derived from human Mitofusin 2.

Uniprot:
遺伝子(ID):
発現特異性:
O95140 MFN2_HUMAN:

Ubiquitous; expressed at low level. Highly expressed in heart and kidney.

タンパク質配列:
MSLLFSRCNSIVTVKKNKRHMAEVNASPLKHFVTAKKKINGIFEQLGAYIQESATFLEDTYRNAELDPVTTEEQVLDVKGYLSKVRGISEVLARRHMKVAFFGRTSNGKSTVINAMLWDKVLPSGIGHTTNCFLRVEGTDGHEAFLLTEGSEEKRSAKTVNQLAHALHQDKQLHAGSLVSVMWPNSKCPLLKDDLVLMDSPGIDVTTELDSWIDKFCLDADVFVLVANSESTLMQTEKHFFHKVSERLSRPNIFILNNRWDASASEPEYMEEVRRQHMERCTSFLVDELGVVDRSQAGDRIFFVSAKEVLNARIQKAQGMPEGGGALAEGFQVRMFEFQNFERRFEECISQSAVKTKFEQHTVRAKQIAEAVRLIMDSLHMAAREQQVYCEEMREERQDRLKFIDKQLELLAQDYKLRIKQITEEVERQVSTAMAEEIRRLSVLVDDYQMDFHPSPVVLKVYKNELHRHIEEGLGRNMSDRCSTAITNSLQTMQQDMIDGLKPLLPVSVRSQIDMLVPRQCFSLNYDLNCDKLCADFQEDIEFHFSLGWTMLVNRFLGPKNSRRALMGYNDQVQRPIPLTPANPSMPPLPQGSLTQEEFMVSMVTGLASLTSRTSMGILVVGGVVWKAVGWRLIALSFGLYGLLYVYERLTWTTKAKERAFKRQFVEHASEKLQLVISYTGSNCSHQVQQELSGTFAHLCQQVDVTRENLEQEIAAMNKKIEVLDSLQSKAKLLRNKAGWLDSELNMFTHQYLQPSR

PTMs - O95140 基板として

Site PTM Type Enzyme
Ubiquitination
S10 Phosphorylation
S27 Phosphorylation P45984 (MAPK9)
K30 Ubiquitination
K36 Acetylation
K36 Ubiquitination
K79 Ubiquitination
K84 Ubiquitination
K98 Ubiquitination
T111 Phosphorylation
K154 Ubiquitination
K158 Ubiquitination
K171 Ubiquitination
K192 Ubiquitination
K243 Acetylation
K243 Ubiquitination
K307 Ubiquitination
K316 Ubiquitination
K355 Acetylation
K355 Ubiquitination
K357 Ubiquitination
K366 Ubiquitination
K406 Ubiquitination
K416 Ubiquitination
K420 Ubiquitination
T423 Phosphorylation
S442 Phosphorylation
K460 Ubiquitination
K463 Ubiquitination
K560 Ubiquitination
K662 Ubiquitination
K719 Ubiquitination
K720 Ubiquitination
K730 Ubiquitination
K737 Ubiquitination
S756 Phosphorylation

研究背景

機能:

Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion. Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events. Overexpression induces the formation of mitochondrial networks. Membrane clustering requires GTPase activity and may involve a major rearrangement of the coiled coil domains (Probable). Plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes (By similarity). Plays an important role in the regulation of vascular smooth muscle cell proliferation (By similarity). Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PRKN recruitment to dysfunctional mitochondria. Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress (By similarity). Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions (By similarity).

PTMs:

Phosphorylated by PINK1.

Ubiquitinated by non-degradative ubiquitin by PRKN, promoting mitochondrial fusion; deubiquitination by USP30 inhibits mitochondrial fusion.

細胞の位置付け:

Mitochondrion outer membrane>Multi-pass membrane protein.
Note: Colocalizes with BAX during apoptosis.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Ubiquitous; expressed at low level. Highly expressed in heart and kidney.

サブユニット構造:

Forms homomultimers and heteromultimers with MFN1. Oligomerization is essential for mitochondrion fusion (Probable). Interacts with VAT1 (By similarity). Interacts with STOML2; may form heterooligomers. Interacts (phosphorylated) with PRKN. Interacts with EIF2AK3 (By similarity).

タンパク質ファミリー:

A helix bundle is formed by helices from the N-terminal and the C-terminal part of the protein. The GTPase domain cannot be expressed by itself, without the helix bundle. Rearrangement of the helix bundle and/or of the coiled coil domains may bring membranes from adjacent mitochondria into close contact, and thereby play a role in mitochondrial fusion.

Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. Mitofusin subfamily.

研究領域

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

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