WEE1 Antibody - #AF6815
| 製品: | WEE1 Antibody |
| カタログ: | AF6815 |
| タンパク質の説明: | Rabbit polyclonal antibody to WEE1 |
| アプリケーション: | IF/ICC |
| 反応性: | Human, Mouse, Rat |
| 分子量: | 55kD(isoform), 75~100kD; 72kD(Calculated). |
| ユニプロット: | P30291 |
| RRID: | AB_2847538 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF6815, RRID:AB_2847538.
折りたたみ／展開
DKFZp686I18166; EC 2.7.10.2; FLJ16446; MGC105683; OTTHUMP00000231338; OTTHUMP00000231339; Wee 1; WEE 1 homolog 1 (S. pombe); WEE1; WEE1 homolog (S. pombe); Wee1 homolog; WEE1 homolog S. pombe; Wee1 like protein kinase; Wee1 tyrosine kinase; Wee1+ homolog; Wee1+ S. pombe homolog; WEE1, S. pombe, homolog of; WEE1, somatic; Wee1-like protein kinase; WEE1_HUMAN; WEE1A; Wee1A kinase; WEE1hu;
免疫原
A synthesized peptide derived from human WEE1.
- P30291 WEE1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSFLSRQQPPPPRRAGAACTLRQKLIFSPCSDCEEEEEEEEEEGSGHSTGEDSAFQEPDSPLPPARSPTEPGPERRRSPGPAPGSPGELEEDLLLPGACPGADEAGGGAEGDSWEEEGFGSSSPVKSPAAPYFLGSSFSPVRCGGPGDASPRGCGARRAGEGRRSPRPDHPGTPPHKTFRKLRLFDTPHTPKSLLSKARGIDSSSVKLRGSSLFMDTEKSGKREFDVRQTPQVNINPFTPDSLLLHSSGQCRRRKRTYWNDSCGEDMEASDYELEDETRPAKRITITESNMKSRYTTEFHELEKIGSGEFGSVFKCVKRLDGCIYAIKRSKKPLAGSVDEQNALREVYAHAVLGQHSHVVRYFSAWAEDDHMLIQNEYCNGGSLADAISENYRIMSYFKEAELKDLLLQVGRGLRYIHSMSLVHMDIKPSNIFISRTSIPNAASEEGDEDDWASNKVMFKIGDLGHVTRISSPQVEEGDSRFLANEVLQENYTHLPKADIFALALTVVCAAGAEPLPRNGDQWHEIRQGRLPRIPQVLSQEFTELLKVMIHPDPERRPSAMALVKHSVLLSASRKSAEQLRIELNAEKFKNSLLQKELKKAQMAKAAAEERALFTDRMATRSTTQSNRTSRLIGKKMNRSVSLTIY
PTMs - P30291 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Phosphorylation | Uniprot | ||
| R14 | Methylation | Uniprot | |
| S48 | Phosphorylation | Uniprot | |
| T49 | Phosphorylation | Uniprot | |
| S53 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S67 | Phosphorylation | Uniprot | |
| T69 | Phosphorylation | Uniprot | |
| S78 | Phosphorylation | Uniprot | |
| S85 | Phosphorylation | Uniprot | |
| S121 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S123 | Phosphorylation | P53350 (PLK1) , P06493 (CDK1) , P24941 (CDK2) | Uniprot |
| S127 | Phosphorylation | Uniprot | |
| Y132 | Phosphorylation | Uniprot | |
| S137 | Phosphorylation | Uniprot | |
| S139 | Phosphorylation | Uniprot | |
| S150 | Phosphorylation | Uniprot | |
| S165 | Phosphorylation | Uniprot | |
| T173 | Phosphorylation | Uniprot | |
| T187 | Phosphorylation | Uniprot | |
| T190 | Phosphorylation | Uniprot | |
| S193 | Phosphorylation | Uniprot | |
| S196 | Phosphorylation | Uniprot | |
| R199 | Methylation | Uniprot | |
| K207 | Ubiquitination | Uniprot | |
| S211 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| S212 | Phosphorylation | P48730 (CSNK1D) | Uniprot |
| T217 | Phosphorylation | Uniprot | |
| T239 | Phosphorylation | Uniprot | |
| S242 | Phosphorylation | Uniprot | |
| Y258 | Phosphorylation | Uniprot | |
| S262 | Phosphorylation | Uniprot | |
| S270 | Phosphorylation | Uniprot | |
| Y272 | Phosphorylation | Uniprot | |
| K292 | Ubiquitination | Uniprot | |
| S293 | Phosphorylation | Uniprot | |
| K304 | Ubiquitination | Uniprot | |
| S307 | Phosphorylation | Uniprot | |
| S312 | Phosphorylation | Uniprot | |
| K315 | Ubiquitination | Uniprot | |
| S444 | Phosphorylation | Uniprot | |
| K460 | Ubiquitination | Uniprot | |
| S472 | Phosphorylation | Uniprot | |
| S480 | Phosphorylation | Uniprot | |
| K547 | Ubiquitination | Uniprot | |
| S571 | Phosphorylation | Uniprot | |
| S576 | Phosphorylation | Uniprot | |
| K588 | Ubiquitination | Uniprot | |
| K590 | Sumoylation | Uniprot | |
| K590 | Ubiquitination | Uniprot | |
| K596 | Ubiquitination | Uniprot | |
| K600 | Ubiquitination | Uniprot | |
| R617 | Methylation | Uniprot | |
| S642 | Phosphorylation | Q8IWQ3 (BRSK2) , Q8TDC3 (BRSK1) , P31749 (AKT1) | Uniprot |
| Y646 | Phosphorylation | Uniprot |
PTMs - P30291 酵素として
研究背景
Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
Phosphorylated during M and G1 phases. Also autophosphorylated. Phosphorylation at Ser-642 by BRSK1 and BRSK2 in post-mitotic neurons, leads to down-regulate WEE1 activity in polarized neurons. Phosphorylated at Ser-53 and Ser-123 by PLK1 and CDK1, respectively, generating an signal for degradation that can be recognized by the SCF(BTRC) complex, leading to its ubiquitination and degradation at the onset of G2/M phase.
Dephosphorylated at Thr-239 by CTDP1.
Ubiquitinated and degraded at the onset of G2/M phase.
Nucleus.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.
研究領域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
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