ZEB2 Antibody - #AF6840

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製品: ZEB2 Antibody
カタログ: AF6840
タンパク質の説明: Rabbit polyclonal antibody to ZEB2
アプリケーション: IF/ICC
反応性: Human, Mouse, Rat
分子量: 136~200kD; 136kD(Calculated).
ユニプロット: O60315
RRID: AB_2847563

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関連ダウンロード
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
ZEB2 Antibody detects endogenous levels of total ZEB2.
RRID:
AB_2847563
引用形式: Affinity Biosciences Cat# AF6840, RRID:AB_2847563.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

FLJ42816; HSPC082; KIAA0569; SIP 1; SIP1; Smad Interacting Protein 1; Smad-interacting protein 1; SMADIP 1; SMADIP1; ZEB 2; Zeb2; ZEB2_HUMAN; Zfhx1b; ZFHX1B protein; Zfx1b; Zinc finger E box binding protein 2; Zinc finger E-box-binding homeobox 2; Zinc finger homeobox 1b; zinc finger homeobox protein 1; Zinc finger homeobox protein 1b;

免疫原

免疫原:

A synthesized peptide derived from human ZEB2.

Uniprot:

O60315(ZEB2_HUMAN) >>Visit HPA database.

遺伝子(ID):
ZEB2(9839)
タンパク質配列:
MKQPIMADGPRCKRRKQANPRRKNVVNYDNVVDTGSETDEEDKLHIAEDDGIANPLDQETSPASVPNHESSPHVSQALLPREEEEDEIREGGVEHPWHNNEILQASVDGPEEMKEDYDTMGPEATIQTAINNGTVKNANCTSDFEEYFAKRKLEERDGHAVSIEEYLQRSDTAIIYPEAPEELSRLGTPEANGQEENDLPPGTPDAFAQLLTCPYCDRGYKRLTSLKEHIKYRHEKNEENFSCPLCSYTFAYRTQLERHMVTHKPGTDQHQMLTQGAGNRKFKCTECGKAFKYKHHLKEHLRIHSGEKPYECPNCKKRFSHSGSYSSHISSKKCIGLISVNGRMRNNIKTGSSPNSVSSSPTNSAITQLRNKLENGKPLSMSEQTGLLKIKTEPLDFNDYKVLMATHGFSGTSPFMNGGLGATSPLGVHPSAQSPMQHLGVGMEAPLLGFPTMNSNLSEVQKVLQIVDNTVSRQKMDCKAEEISKLKGYHMKDPCSQPEEQGVTSPNIPPVGLPVVSHNGATKSIIDYTLEKVNEAKACLQSLTTDSRRQISNIKKEKLRTLIDLVTDDKMIENHNISTPFSCQFCKESFPGPIPLHQHERYLCKMNEEIKAVLQPHENIVPNKAGVFVDNKALLLSSVLSEKGMTSPINPYKDHMSVLKAYYAMNMEPNSDELLKISIAVGLPQEFVKEWFEQRKVYQYSNSRSPSLERSSKPLAPNSNPPTKDSLLPRSPVKPMDSITSPSIAELHNSVTNCDPPLRLTKPSHFTNIKPVEKLDHSRSNTPSPLNLSSTSSKNSHSSSYTPNSFSSEELQAEPLDLSLPKQMKEPKSIIATKNKTKASSISLDHNSVSSSSENSDEPLNLTFIKKEFSNSNNLDNKSTNPVFSMNPFSAKPLYTALPPQSAFPPATFMPPVQTSIPGLRPYPGLDQMSFLPHMAYTYPTGAATFADMQQRRKYQRKQGFQGELLDGAQDYMSGLDDMTDSDSCLSRKKIKKTESGMYACDLCDKTFQKSSSLLRHKYEHTGKRPHQCQICKKAFKHKHHLIEHSRLHSGEKPYQCDKCGKRFSHSGSYSQHMNHRYSYCKREAEEREAAEREAREKGHLEPTELLMNRAYLQSITPQGYSDSEERESMPRDGESEKEHEKEGEDGYGKLGRQDGDEEFEEEEEESENKSMDTDPETIRDEEETGDHSMDDSSEDGKMETKSDHEEDNMEDGM

PTMs - O60315 基板として

Site PTM Type Enzyme
R11 Methylation
T34 Phosphorylation
S36 Phosphorylation
T38 Phosphorylation
S64 Phosphorylation
T119 Phosphorylation
S142 Phosphorylation
Y147 Phosphorylation
S162 Phosphorylation
S184 Phosphorylation
T188 Phosphorylation
T254 Phosphorylation
T274 Phosphorylation
T285 Phosphorylation
S305 Phosphorylation
S324 Phosphorylation
S327 Phosphorylation
S330 Phosphorylation
S331 Phosphorylation
T350 Phosphorylation
S352 Phosphorylation
S353 Phosphorylation
S356 Phosphorylation
S358 Phosphorylation
S359 Phosphorylation
S360 Phosphorylation
T362 Phosphorylation
K377 Acetylation
K391 Sumoylation
S410 Phosphorylation
S431 Phosphorylation
T470 Phosphorylation
S505 Phosphorylation
K632 Ubiquitination
S641 Phosphorylation
S647 Phosphorylation
S703 Phosphorylation
S705 Phosphorylation
S707 Phosphorylation
S726 Phosphorylation
S731 Phosphorylation
S741 Phosphorylation
S778 Phosphorylation
S780 Phosphorylation
T782 Phosphorylation
S784 Phosphorylation
S796 Phosphorylation
T802 Phosphorylation
S805 Phosphorylation
S819 Phosphorylation
S829 Phosphorylation
T837 Phosphorylation
S840 Phosphorylation
S841 Phosphorylation
S843 Phosphorylation
S848 Phosphorylation
S853 Phosphorylation
K866 Sumoylation
S1050 Phosphorylation
S1115 Phosphorylation
T1117 Phosphorylation
S1122 Phosphorylation
S1124 Phosphorylation
S1129 Phosphorylation
S1136 Phosphorylation
S1167 Phosphorylation
T1185 Phosphorylation
S1189 Phosphorylation
S1193 Phosphorylation
S1194 Phosphorylation
S1203 Phosphorylation

研究背景

機能:

Transcriptional inhibitor that binds to DNA sequence 5'-CACCT-3' in different promoters. Represses transcription of E-cadherin.

PTMs:

Sumoylation on Lys-391 and Lys-866 is promoted by the E3 SUMO-protein ligase CBX4, and impairs interaction with CTBP1 and transcription repression activity.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Binds activated SMAD1, activated SMAD2 and activated SMAD3; binding with SMAD4 is not detected (By similarity). Interacts with CBX4 and CTBP1.

タンパク質ファミリー:

Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.

研究領域

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

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