Transferrin Receptor Antibody | Affinity Biosciences

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製品:	Transferrin Receptor Antibody
カタログ:	AF6896
タンパク質の説明:	Rabbit polyclonal antibody to Transferrin Receptor
アプリケーション:	IF/ICC
反応性:	Human, Mouse
分子量:	90kD; 85kD(Calculated).
ユニプロット:	P02786
RRID:	AB_2847619

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サイズ	価格	在庫状況
100ul	$280	在庫あり
200ul	$350	在庫あり

プロトコル

製品説明

ソース:

Rabbit

アプリケーション:

IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:

Human,Mouse

クローナリティ:

Polyclonal

特異性:

Transferrin Receptor Antibody detects endogenous levels of total Transferrin Receptor.

RRID:

AB_2847619
引用形式: Affinity Biosciences Cat# AF6896, RRID:AB_2847619.

コンジュゲート:

Unconjugated.

精製:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

別名:

折りたたみ／展開

CD 71; CD71; CD71 antigen; IMD46; OTTHUMP00000208523; OTTHUMP00000208524; OTTHUMP00000208525; p90; sTfR; T9; TFR 1; TfR; TfR1; TFR1_HUMAN; TFRC; TR; Transferrin receptor (p90 CD71); Transferrin receptor protein 1, serum form; Trfr;

免疫原

免疫原:

A synthesized peptide derived from human Transferrin Receptor.

Uniprot:

P02786(TFR1_HUMAN) >>Visit HPA database.

遺伝子(ID):

TFRC(7037)

タンパク質配列:

P02786 TFR1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MMDQARSAFSNLFGGEPLSYTRFSLARQVDGDNSHVEMKLAVDEEENADNNTKANVTKPKRCSGSICYGTIAVIVFFLIGFMIGYLGYCKGVEPKTECERLAGTESPVREEPGEDFPAARRLYWDDLKRKLSEKLDSTDFTGTIKLLNENSYVPREAGSQKDENLALYVENQFREFKLSKVWRDQHFVKIQVKDSAQNSVIIVDKNGRLVYLVENPGGYVAYSKAATVTGKLVHANFGTKKDFEDLYTPVNGSIVIVRAGKITFAEKVANAESLNAIGVLIYMDQTKFPIVNAELSFFGHAHLGTGDPYTPGFPSFNHTQFPPSRSSGLPNIPVQTISRAAAEKLFGNMEGDCPSDWKTDSTCRMVTSESKNVKLTVSNVLKEIKILNIFGVIKGFVEPDHYVVVGAQRDAWGPGAAKSGVGTALLLKLAQMFSDMVLKDGFQPSRSIIFASWSAGDFGSVGATEWLEGYLSSLHLKAFTYINLDKAVLGTSNFKVSASPLLYTLIEKTMQNVKHPVTGQFLYQDSNWASKVEKLTLDNAAFPFLAYSGIPAVSFCFCEDTDYPYLGTTMDTYKELIERIPELNKVARAAAEVAGQFVIKLTHDVELNLDYERYNSQLLSFVRDLNQYRADIKEMGLSLQWLYSARGDFFRATSRLTTDFGNAEKTDRFVMKKLNDRVMRVEYHFLSPYVSPKESPFRHVFWGSGSHTLPALLENLKLRKQNNGAFNETLFRNQLALATWTIQGAANALSGDVWDIDNEF

PTMs - P02786 基板として

P02786

Site	PTM Type	Enzyme	Source
S7	Phosphorylation		Uniprot
S10	Phosphorylation		Uniprot
S19	Phosphorylation		Uniprot
Y20	Phosphorylation	P12931 (SRC)	Uniprot
T21	Phosphorylation		Uniprot
S24	Phosphorylation	P17252 (PRKCA)	Uniprot
S34	Phosphorylation		Uniprot
K39	Ubiquitination		Uniprot
T52	Phosphorylation		Uniprot
K53	Acetylation		Uniprot
K53	Ubiquitination		Uniprot
K58	Ubiquitination		Uniprot
K60	Ubiquitination		Uniprot
T104	O-Glycosylation		Uniprot
S106	Phosphorylation		Uniprot
Y123	Phosphorylation		Uniprot
K128	Acetylation		Uniprot
K128	Ubiquitination		Uniprot
K134	Ubiquitination		Uniprot
K145	Ubiquitination		Uniprot
K161	Ubiquitination		Uniprot
Y168	Phosphorylation		Uniprot
K177	Ubiquitination		Uniprot
K189	Ubiquitination		Uniprot
K193	Ubiquitination		Uniprot
S195	Phosphorylation		Uniprot
S199	Phosphorylation		Uniprot
K205	Ubiquitination		Uniprot
Y219	Phosphorylation		Uniprot
K224	Ubiquitination		Uniprot
K231	Ubiquitination		Uniprot
K240	Ubiquitination		Uniprot
K241	Ubiquitination		Uniprot
N251	N-Glycosylation		Uniprot
K261	Ubiquitination		Uniprot
Y282	Phosphorylation		Uniprot
T286	Phosphorylation		Uniprot
N317	N-Glycosylation		Uniprot
S326	Phosphorylation		Uniprot
S327	Phosphorylation		Uniprot
S338	Phosphorylation		Uniprot
K344	Ubiquitination		Uniprot
K358	Ubiquitination		Uniprot
K371	Ubiquitination		Uniprot
K374	Ubiquitination		Uniprot
K382	Ubiquitination		Uniprot
K385	Ubiquitination		Uniprot
K394	Acetylation		Uniprot
Y402	Phosphorylation		Uniprot
K418	Acetylation		Uniprot
K418	Ubiquitination		Uniprot
S419	Phosphorylation		Uniprot
Y481	Phosphorylation		Uniprot
K486	Ubiquitination		Uniprot
S499	Phosphorylation		Uniprot
Y503	Phosphorylation		Uniprot
K508	Ubiquitination		Uniprot
K514	Ubiquitination		Uniprot
Y523	Phosphorylation		Uniprot
K531	Ubiquitination		Uniprot
T572	Phosphorylation		Uniprot
Y573	Phosphorylation		Uniprot
K585	Ubiquitination		Uniprot
Y611	Phosphorylation		Uniprot
S616	Phosphorylation		Uniprot
S620	Phosphorylation		Uniprot
T658	Phosphorylation		Uniprot
K665	Ubiquitination		Uniprot
Y683	Phosphorylation		Uniprot
S687	Phosphorylation		Uniprot
S691	Phosphorylation		Uniprot
K693	Ubiquitination		Uniprot
K717	Ubiquitination		Uniprot
K720	Ubiquitination		Uniprot

研究背景

P02786_TFR1_HUMAN

機能:

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake.

(Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.

PTMs:

N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.

Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).

Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.

細胞の位置付け:

Cell membrane>Single-pass type II membrane protein. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Secreted.

サブユニット構造:

Homodimer; disulfide-linked. Binds one transferrin or HFE molecule per subunit. Binds the HLA class II histocompatibility antigen, DR1. Interacts with SH3BP3. Interacts with STEAP3; facilitates TFRC endocytosis in erythroid precursor cells.

(Microbial infection) Interacts with Guanarito, Junin and Machupo arenavirus glycoprotein complex.

タンパク質ファミリー:

Belongs to the peptidase M28 family. M28B subfamily.

研究領域

· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)

· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)

· Cellular Processes > Cell growth and death > Ferroptosis. (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)

· Organismal Systems > Immune system > Hematopoietic cell lineage. (View pathway)

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Transferrin Receptor Antibody - #AF6896

関連製品情報

関連ダウンロード

MSDS

説明書

COA

プロトコル

WB Handbook

IHC Protocol

IF/ICC Protocol

製品説明

免疫原

PTMs - P02786 基板として

研究背景

研究領域

Restrictive clause