Phospho-BRCA2 (Ser3291) Antibody - #AF3914

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製品: Phospho-BRCA2 (Ser3291) Antibody
カタログ: AF3914
タンパク質の説明: Rabbit polyclonal antibody to Phospho-BRCA2 (Ser3291)
アプリケーション: IF/ICC
反応性: Human, Mouse, Rat
分子量: 384kD(Calculated).
ユニプロット: P51587
RRID: AB_2847637

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製品説明

ソース:
Rabbit
アプリケーション:
IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
Phospho-BRCA2 (Ser3291) Antibody detects endogenous levels of BRCA2 only when phosphorylated at Ser3291.
RRID:
AB_2847637
引用形式: Affinity Biosciences Cat# AF3914, RRID:AB_2847637.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

BRCA 2; BRCA1/BRCA2 containing complex subunit 2; Brca2; BRCA2, DNA repair associated; BRCA2_HUMAN; BRCC 2; BRCC2; Breast and ovarian cancer susceptibility gene early onset; breast and ovarian cancer susceptibility protein 2; Breast cancer 2 early onset; Breast Cancer 2 tumor suppressor; Breast cancer susceptibility protein BRCA2; Breast cancer type 2 susceptibility protein; BROVCA2; FACD; FAD 1; FAD; FAD1; FANCB; FANCD 1; FANCD; FANCD1; FANCD1 gene; Fanconi anemia complementation group D1; Fanconi anemia group D1 protein; GLM3; mutant BRCA2; OTTHUMP00000018803; OTTHUMP00000042401; PNCA2; XRCC11;

免疫原

免疫原:

A synthesized peptide derived from human BRCA2 around the phosphorylation site of Ser3291.

Uniprot:

P51587(BRCA2_HUMAN) >>Visit HPA database.

遺伝子(ID):
BRCA2(675)
発現特異性:
P51587 BRCA2_HUMAN:

Highest levels of expression in breast and thymus, with slightly lower levels in lung, ovary and spleen.

タンパク質配列:
MPIGSKERPTFFEIFKTRCNKADLGPISLNWFEELSSEAPPYNSEPAEESEHKNNNYEPNLFKTPQRKPSYNQLASTPIIFKEQGLTLPLYQSPVKELDKFKLDLGRNVPNSRHKSLRTVKTKMDQADDVSCPLLNSCLSESPVVLQCTHVTPQRDKSVVCGSLFHTPKFVKGRQTPKHISESLGAEVDPDMSWSSSLATPPTLSSTVLIVRNEEASETVFPHDTTANVKSYFSNHDESLKKNDRFIASVTDSENTNQREAASHGFGKTSGNSFKVNSCKDHIGKSMPNVLEDEVYETVVDTSEEDSFSLCFSKCRTKNLQKVRTSKTRKKIFHEANADECEKSKNQVKEKYSFVSEVEPNDTDPLDSNVANQKPFESGSDKISKEVVPSLACEWSQLTLSGLNGAQMEKIPLLHISSCDQNISEKDLLDTENKRKKDFLTSENSLPRISSLPKSEKPLNEETVVNKRDEEQHLESHTDCILAVKQAISGTSPVASSFQGIKKSIFRIRESPKETFNASFSGHMTDPNFKKETEASESGLEIHTVCSQKEDSLCPNLIDNGSWPATTTQNSVALKNAGLISTLKKKTNKFIYAIHDETSYKGKKIPKDQKSELINCSAQFEANAFEAPLTFANADSGLLHSSVKRSCSQNDSEEPTLSLTSSFGTILRKCSRNETCSNNTVISQDLDYKEAKCNKEKLQLFITPEADSLSCLQEGQCENDPKSKKVSDIKEEVLAAACHPVQHSKVEYSDTDFQSQKSLLYDHENASTLILTPTSKDVLSNLVMISRGKESYKMSDKLKGNNYESDVELTKNIPMEKNQDVCALNENYKNVELLPPEKYMRVASPSRKVQFNQNTNLRVIQKNQEETTSISKITVNPDSEELFSDNENNFVFQVANERNNLALGNTKELHETDLTCVNEPIFKNSTMVLYGDTGDKQATQVSIKKDLVYVLAEENKNSVKQHIKMTLGQDLKSDISLNIDKIPEKNNDYMNKWAGLLGPISNHSFGGSFRTASNKEIKLSEHNIKKSKMFFKDIEEQYPTSLACVEIVNTLALDNQKKLSKPQSINTVSAHLQSSVVVSDCKNSHITPQMLFSKQDFNSNHNLTPSQKAEITELSTILEESGSQFEFTQFRKPSYILQKSTFEVPENQMTILKTTSEECRDADLHVIMNAPSIGQVDSSKQFEGTVEIKRKFAGLLKNDCNKSASGYLTDENEVGFRGFYSAHGTKLNVSTEALQKAVKLFSDIENISEETSAEVHPISLSSSKCHDSVVSMFKIENHNDKTVSEKNNKCQLILQNNIEMTTGTFVEEITENYKRNTENEDNKYTAASRNSHNLEFDGSDSSKNDTVCIHKDETDLLFTDQHNICLKLSGQFMKEGNTQIKEDLSDLTFLEVAKAQEACHGNTSNKEQLTATKTEQNIKDFETSDTFFQTASGKNISVAKESFNKIVNFFDQKPEELHNFSLNSELHSDIRKNKMDILSYEETDIVKHKILKESVPVGTGNQLVTFQGQPERDEKIKEPTLLGFHTASGKKVKIAKESLDKVKNLFDEKEQGTSEITSFSHQWAKTLKYREACKDLELACETIEITAAPKCKEMQNSLNNDKNLVSIETVVPPKLLSDNLCRQTENLKTSKSIFLKVKVHENVEKETAKSPATCYTNQSPYSVIENSALAFYTSCSRKTSVSQTSLLEAKKWLREGIFDGQPERINTADYVGNYLYENNSNSTIAENDKNHLSEKQDTYLSNSSMSNSYSYHSDEVYNDSGYLSKNKLDSGIEPVLKNVEDQKNTSFSKVISNVKDANAYPQTVNEDICVEELVTSSSPCKNKNAAIKLSISNSNNFEVGPPAFRIASGKIVCVSHETIKKVKDIFTDSFSKVIKENNENKSKICQTKIMAGCYEALDDSEDILHNSLDNDECSTHSHKVFADIQSEEILQHNQNMSGLEKVSKISPCDVSLETSDICKCSIGKLHKSVSSANTCGIFSTASGKSVQVSDASLQNARQVFSEIEDSTKQVFSKVLFKSNEHSDQLTREENTAIRTPEHLISQKGFSYNVVNSSAFSGFSTASGKQVSILESSLHKVKGVLEEFDLIRTEHSLHYSPTSRQNVSKILPRVDKRNPEHCVNSEMEKTCSKEFKLSNNLNVEGGSSENNHSIKVSPYLSQFQQDKQQLVLGTKVSLVENIHVLGKEQASPKNVKMEIGKTETFSDVPVKTNIEVCSTYSKDSENYFETEAVEIAKAFMEDDELTDSKLPSHATHSLFTCPENEEMVLSNSRIGKRRGEPLILVGEPSIKRNLLNEFDRIIENQEKSLKASKSTPDGTIKDRRLFMHHVSLEPITCVPFRTTKERQEIQNPNFTAPGQEFLSKSHLYEHLTLEKSSSNLAVSGHPFYQVSATRNEKMRHLITTGRPTKVFVPPFKTKSHFHRVEQCVRNINLEENRQKQNIDGHGSDDSKNKINDNEIHQFNKNNSNQAAAVTFTKCEEEPLDLITSLQNARDIQDMRIKKKQRQRVFPQPGSLYLAKTSTLPRISLKAAVGGQVPSACSHKQLYTYGVSKHCIKINSKNAESFQFHTEDYFGKESLWTGKGIQLADGGWLIPSNDGKAGKEEFYRALCDTPGVDPKLISRIWVYNHYRWIIWKLAAMECAFPKEFANRCLSPERVLLQLKYRYDTEIDRSRRSAIKKIMERDDTAAKTLVLCVSDIISLSANISETSSNKTSSADTQKVAIIELTDGWYAVKAQLDPPLLAVLKNGRLTVGQKIILHGAELVGSPDACTPLEAPESLMLKISANSTRPARWYTKLGFFPDPRPFPLPLSSLFSDGGNVGCVDVIIQRAYPIQWMEKTSSGLYIFRNEREEEKEAAKYVEAQQKRLEALFTKIQEEFEEHEENTTKPYLPSRALTRQQVRALQDGAELYEAVKNAADPAYLEGYFSEEQLRALNNHRQMLNDKKQAQIQLEIRKAMESAEQKEQGLSRDVTTVWKLRIVSYSKKEKDSVILSIWRPSSDLYSLLTEGKRYRIYHLATSKSKSKSERANIQLAATKKTQYQQLPVSDEILFQIYQPREPLHFSKFLDPDFQPSCSEVDLIGFVVSVVKKTGLAPFVYLSDECYNLLAIKFWIDLNEDIIKPHMLIAASNLQWRPESKSGLLTLFAGDFSVFSASPKEGHFQETFNKMKNTVENIDILCNEAENKLMHILHANDPKWSTPTKDCTSGPYTAQIIPGTGNKLLMSSPNCEIYYQSPLSLCMAKRKSVSTPVSAQMTSKSCKGEKEIDDQKNCKKRRALDFLSRLPLPPPVSPICTFVSPAAQKAFQPPRSCGTKYETPIKKKELNSPQMTPFKKFNEISLLESNSIADEELALINTQALLSGSTGEKQFISVSESTRTAPTSSEDYLRLKRRCTTSLIKEQESSQASTEECEKNKQDTITTKKYI

PTMs - P51587 基板として

Site PTM Type Enzyme
Y57 Phosphorylation
K63 Ubiquitination
K68 Ubiquitination
S70 Phosphorylation
Y71 Phosphorylation
S76 Phosphorylation
T77 Phosphorylation
S93 Phosphorylation
K96 Ubiquitination
T167 Phosphorylation
S193 Phosphorylation P53350 (PLK1)
T203 Phosphorylation P53350 (PLK1)
S205 Phosphorylation P53350 (PLK1)
S206 Phosphorylation P53350 (PLK1)
T207 Phosphorylation P53350 (PLK1)
K230 Ubiquitination
S239 Phosphorylation P53350 (PLK1)
K241 Ubiquitination
S253 Phosphorylation
K268 Methylation
K268 Ubiquitination
S273 Phosphorylation
K275 Methylation
K343 Ubiquitination
T363 Phosphorylation
K382 Ubiquitination
S384 Phosphorylation
K426 Ubiquitination
T431 Phosphorylation
S445 Phosphorylation
S451 Phosphorylation
K454 Acetylation
K457 Ubiquitination
K485 Ubiquitination
S492 Phosphorylation
K502 Ubiquitination
S511 Phosphorylation
S519 Phosphorylation
S547 Phosphorylation
S581 Phosphorylation
T582 Phosphorylation
Y592 Phosphorylation
K601 Ubiquitination
K610 Ubiquitination
T675 Phosphorylation
S677 Phosphorylation
T680 Phosphorylation
S683 Phosphorylation
Y688 Phosphorylation
K745 Ubiquitination
S755 Phosphorylation
K757 Ubiquitination
K799 Ubiquitination
S805 Phosphorylation
K811 Ubiquitination
K817 Ubiquitination
K829 Ubiquitination
K838 Ubiquitination
K848 Ubiquitination
K862 Ubiquitination
S879 Phosphorylation
S884 Phosphorylation
K907 Ubiquitination
K945 Ubiquitination
Y949 Phosphorylation
K964 Ubiquitination
S973 Phosphorylation
S976 Phosphorylation
K981 Ubiquitination
K985 Ubiquitination
K992 Ubiquitination
K1094 Ubiquitination
T1104 Phosphorylation
K1108 Ubiquitination
K1132 Ubiquitination
Y1135 Phosphorylation
K1139 Ubiquitination
K1153 Ubiquitination
K1197 Ubiquitination
K1202 Ubiquitination
K1226 Ubiquitination
K1239 Ubiquitination
K1264 Ubiquitination
T1282 Phosphorylation
K1323 Ubiquitination
S1331 Phosphorylation
K1343 Ubiquitination
K1374 Ubiquitination
K1381 Ubiquitination
K1394 Ubiquitination
K1406 Ubiquitination
K1413 Sumoylation
K1413 Ubiquitination
K1419 Ubiquitination
K1434 Ubiquitination
K1440 Ubiquitination
K1453 Ubiquitination
K1474 Acetylation
K1474 Ubiquitination
Y1480 Phosphorylation
K1492 Ubiquitination
K1515 Ubiquitination
K1517 Ubiquitination
S1528 Phosphorylation
K1533 Acetylation
K1536 Acetylation
K1543 Ubiquitination
K1549 Ubiquitination
K1565 Ubiquitination
K1614 Ubiquitination
T1624 Phosphorylation
K1631 Ubiquitination
S1632 Phosphorylation
K1678 Ubiquitination
S1680 Phosphorylation
K1690 Ubiquitination
K1729 Ubiquitination
K1765 Ubiquitination
K1767 Ubiquitination
S1770 Phosphorylation
K1777 Ubiquitination
K1783 Ubiquitination
K1789 Ubiquitination
S1818 Phosphorylation
K1860 Ubiquitination
K1863 Ubiquitination
S1869 Phosphorylation
K1872 Ubiquitination
S1926 Phosphorylation
K1941 Ubiquitination
S1943 Phosphorylation
K1944 Ubiquitination
S1946 Phosphorylation
S1951 Phosphorylation
K1959 Ubiquitination
K1967 Ubiquitination
S1970 Phosphorylation
K1984 Ubiquitination
K2008 Ubiquitination
K2013 Ubiquitination
K2017 Methylation
K2017 Ubiquitination
T2031 Phosphorylation
T2035 Phosphorylation
S2041 Phosphorylation
S2072 Phosphorylation
K2075 Ubiquitination
K2077 Ubiquitination
S2091 Phosphorylation
S2095 Phosphorylation
K2104 Ubiquitination
K2124 Ubiquitination
K2150 Ubiquitination
S2152 Phosphorylation
S2156 Phosphorylation Q13535 (ATR)
K2162 Ubiquitination
S2186 Phosphorylation
K2191 Ubiquitination
K2196 Ubiquitination
K2206 Ubiquitination
K2244 Ubiquitination
K2286 Ubiquitination
K2302 Ubiquitination
K2308 Ubiquitination
K2359 Ubiquitination
Y2363 Phosphorylation
K2404 Ubiquitination
K2411 Methylation
K2411 Ubiquitination
K2413 Methylation
K2448 Ubiquitination
K2459 Ubiquitination
K2472 Ubiquitination
K2498 Acetylation
K2514 Ubiquitination
K2524 Methylation
K2538 Ubiquitination
K2570 Ubiquitination
K2577 Ubiquitination
K2594 Ubiquitination
K2597 Ubiquitination
K2640 Ubiquitination
K2657 Ubiquitination
K2741 Ubiquitination
K2882 Ubiquitination
Y2905 Phosphorylation
K2950 Methylation
S2954 Phosphorylation
K2958 Ubiquitination
T2967 Phosphorylation
T2968 Phosphorylation
K3031 Ubiquitination
K3179 Ubiquitination
T3193 Phosphorylation
K3196 Ubiquitination
T3199 Phosphorylation
T3211 Phosphorylation
S3231 Phosphorylation
K3238 Ubiquitination
S3239 Phosphorylation
T3242 Phosphorylation
S3250 Phosphorylation
S3284 Phosphorylation
S3291 Phosphorylation P06493 (CDK1)
T3306 Phosphorylation
S3319 Phosphorylation
T3323 Phosphorylation
T3387 Phosphorylation PR:O14757 (hCHEK1) , PR:O96017 (hCHEK2)
S3397 Phosphorylation

研究背景

機能:

Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA). Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. Part of a PALB2-scaffolded HR complex containing RAD51C and which is thought to play a role in DNA repair by HR. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks; together with PALB2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity. In concert with NPM1, regulates centrosome duplication. Interacts with the TREX-2 complex (transcription and export complex 2) subunits PCID2 and SEM1, and is required to prevent R-loop-associated DNA damage and thus transcription-associated genomic instability. Silencing of BRCA2 promotes R-loop accumulation at actively transcribed genes in replicating and non-replicating cells, suggesting that BRCA2 mediates the control of R-loop associated genomic instability, independently of its known role in homologous recombination.

PTMs:

Phosphorylated by ATM upon irradiation-induced DNA damage. Phosphorylation by CHEK1 and CHEK2 regulates interaction with RAD51. Phosphorylation at Ser-3291 by CDK1 and CDK2 is low in S phase when recombination is active, but increases as cells progress towards mitosis; this phosphorylation prevents homologous recombination-dependent repair during S phase and G2 by inhibiting RAD51 binding.

Ubiquitinated in the absence of DNA damage; this does not lead to proteasomal degradation. In contrast, ubiquitination in response to DNA damage leads to proteasomal degradation.

細胞の位置付け:

Nucleus. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Highest levels of expression in breast and thymus, with slightly lower levels in lung, ovary and spleen.

サブユニット構造:

Monomer and dimer. Interacts with RAD51; regulates RAD51 recruitment and function at sites of DNA repair. Interacts with WDR16, USP11, DMC1, ROCK2 and NPM1. Interacts with SEM1; the interaction masks a nuclear export signal in BRCA2. Interacts with both nonubiquitinated and monoubiquitinated FANCD2; this complex also includes XRCC3 and phosphorylated FANCG. Part of a BRCA complex containing BRCA1, BRCA2 and PALB2. Interacts directly with PALB2 which may serve as a scaffold for a HR complex containing PALB2, BRCA2, RAD51C, RAD51 and XRCC3. Interacts with BRCA1 only in the presence of PALB2 which serves as the bridging protein. Interacts with POLH; the interaction is direct. Interacts with the TREX-2 complex subunits PCID2 and SEM1.

研究領域

· Genetic Information Processing > Replication and repair > Homologous recombination.

· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

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