Phospho-WWOX (Tyr33) Antibody - #AF3928
| 製品: | Phospho-WWOX (Tyr33) Antibody |
| カタログ: | AF3928 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-WWOX (Tyr33) |
| アプリケーション: | ELISA(peptide) |
| 反応性: | Human, Mouse |
| 分子量: | 47kD(Calculated). |
| ユニプロット: | Q9NZC7 |
| RRID: | AB_2847651 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF3928, RRID:AB_2847651.
免疫原
A synthesized peptide derived from human WWOX around the phosphorylation site of Tyr33.
Widely expressed. Strongly expressed in testis, prostate, and ovary. Overexpressed in cancer cell lines. Isoform 5 and isoform 6 may only be expressed in tumor cell lines.
- Q9NZC7 WWOX_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAALRYAGLDDTDSEDELPPGWEERTTKDGWVYYANHTEEKTQWEHPKTGKRKRVAGDLPYGWEQETDENGQVFFVDHINKRTTYLDPRLAFTVDDNPTKPTTRQRYDGSTTAMEILQGRDFTGKVVVVTGANSGIGFETAKSFALHGAHVILACRNMARASEAVSRILEEWHKAKVEAMTLDLALLRSVQHFAEAFKAKNVPLHVLVCNAATFALPWSLTKDGLETTFQVNHLGHFYLVQLLQDVLCRSAPARVIVVSSESHRFTDINDSLGKLDFSRLSPTKNDYWAMLAYNRSKLCNILFSNELHRRLSPRGVTSNAVHPGNMMYSNIHRSWWVYTLLFTLARPFTKSMQQGAATTVYCAAVPELEGLGGMYFNNCCRCMPSPEAQSEETARTLWALSERLIQERLGSQSG
PTMs - Q9NZC7 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y6 | Phosphorylation | Uniprot | |
| T12 | Phosphorylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| K28 | Ubiquitination | Uniprot | |
| Y33 | Phosphorylation | P12931 (SRC) | Uniprot |
| K48 | Ubiquitination | Uniprot | |
| Y61 | Phosphorylation | Uniprot | |
| K100 | Ubiquitination | Uniprot | |
| T111 | Phosphorylation | Uniprot | |
| S134 | Phosphorylation | Uniprot | |
| S162 | Phosphorylation | Uniprot | |
| K274 | Ubiquitination | Uniprot | |
| Y287 | Phosphorylation | Q07912 (TNK2) | Uniprot |
| S312 | Phosphorylation | Uniprot |
研究背景
Putative oxidoreductase. Acts as a tumor suppressor and plays a role in apoptosis. Required for normal bone development (By similarity). May function synergistically with p53/TP53 to control genotoxic stress-induced cell death. Plays a role in TGFB1 signaling and TGFB1-mediated cell death. May also play a role in tumor necrosis factor (TNF)-mediated cell death. Inhibits Wnt signaling, probably by sequestering DVL2 in the cytoplasm.
Phosphorylated upon genotoxic stress. Phosphorylation of Tyr-33 regulates interaction with TP53, TP73 and MAPK8. May also regulate proapoptotic activity. Phosphorylation by TNK2 is associated with polyubiquitination and degradation.
Ubiquitinated when phosphorylated by TNK2, leading to its degradation.
Cytoplasm. Nucleus. Mitochondrion. Golgi apparatus.
Note: Partially localizes to the mitochondria (PubMed:14695174). Translocates to the nucleus upon genotoxic stress or TNF stimulation (By similarity). Translocates to the nucleus in response to TGFB1 (PubMed:19366691). Isoform 5 and isoform 6 may localize in the nucleus.
Widely expressed. Strongly expressed in testis, prostate, and ovary. Overexpressed in cancer cell lines. Isoform 5 and isoform 6 may only be expressed in tumor cell lines.
Interacts with TP53, p73/TP73 and MAPK8. Interacts with MAPT/TAU, RUNX2 and HYAL2 (By similarity). Forms a ternary complex with TP53 and MDM2. Interacts with ERBB4, LITAF and WBP1. Interacts with DVL1, DVL2 and DVL3. May interact with FAM189B and SCOTIN. Interacts with TNK2. Interacts with TMEM207.
The WW 1 domain mediates interaction with TP53, and probably TP73, TFAP2C, LITAF and WBP1.
Belongs to the short-chain dehydrogenases/reductases (SDR) family.
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