Phospho-EPB41 (Tyr660/Tyr418) Antibody - #AF3943
| 製品: | Phospho-EPB41 (Tyr660/Tyr418) Antibody |
| カタログ: | AF3943 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-EPB41 (Tyr660/Tyr418) |
| アプリケーション: | ELISA(peptide) |
| 反応性: | Human, Mouse |
| 分子量: | 97kD(Calculated). |
| ユニプロット: | P11171 |
| RRID: | AB_2847666 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF3943, RRID:AB_2847666.
折りたたみ／展開
4.1R; 41_HUMAN; Band 4.1; E41P; EL 1; EL1; EL1 gene; Elliptocytosis 1; Elliptocytosis 1 RH linked; EPB 4.1; EPB 41; EPB4.1; Epb41; Erythrocyte membrane protein band 4.1 (elliptocytosis 1 RH linked); Erythrocyte membrane protein band 4.1; Erythrocyte surface protein band 4.1; HE; P4.1; Protein 4.1; Protein 4.1, red blood cell type;
免疫原
A synthesized peptide derived from human EPB41 around the phosphorylation site of Tyr660/418.
- P11171 41_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTTEKSLVTEAENSQHQQKEEGEEAINSGQQEPQQEESCQTAAEGDNWCEQKLKASNGDTPTHEDLTKNKERTSESRGLSRLFSSFLKRPKSQVSEEEGKEVESDKEKGEGGQKEIEFGTSLDEEIILKAPIAAPEPELKTDPSLDLHSLSSAETQPAQEELREDPDFEIKEGEGLEECSKIEVKEESPQSKAETELKASQKPIRKHRNMHCKVSLLDDTVYECVVEKHAKGQDLLKRVCEHLNLLEEDYFGLAIWDNATSKTWLDSAKEIKKQVRGVPWNFTFNVKFYPPDPAQLTEDITRYYLCLQLRQDIVAGRLPCSFATLALLGSYTIQSELGDYDPELHGVDYVSDFKLAPNQTKELEEKVMELHKSYRSMTPAQADLEFLENAKKLSMYGVDLHKAKDLEGVDIILGVCSSGLLVYKDKLRINRFPWPKVLKISYKRSSFFIKIRPGEQEQYESTIGFKLPSYRAAKKLWKVCVEHHTFFRLTSTDTIPKSKFLALGSKFRYSGRTQAQTRQASALIDRPAPHFERTASKRASRSLDGAAAVDSADRSPRPTSAPAITQGQVAEGGVLDASAKKTVVPKAQKETVKAEVKKEDEPPEQAEPEPTEAWKVEKTHIEVTVPTSNGDQTQKLAEKTEDLIRMRKKKRERLDGENIYIRHSNLMLEDLDKSQEEIKKHHASISELKKNFMESVPEPRPSEWDKRLSTHSPFRTLNINGQIPTGEGPPLVKTQTVTISDNANAVKSEIPTKDVPIVHTETKTITYEAAQTDDNSGDLDPGVLLTAQTITSETPSSTTTTQITKTVKGGISETRIEKRIVITGDADIDHDQVLVQAIKEAKEQHPDMSVTKVVVHQETEIADE
PTMs - P11171 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S6 | Phosphorylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| S56 | Phosphorylation | Uniprot | |
| T60 | Phosphorylation | Uniprot | |
| T62 | Phosphorylation | Uniprot | |
| R77 | Methylation | Uniprot | |
| S80 | Phosphorylation | Uniprot | |
| S84 | Phosphorylation | Uniprot | |
| S85 | Phosphorylation | Uniprot | |
| S92 | Phosphorylation | Uniprot | |
| S95 | Phosphorylation | Uniprot | |
| S104 | Phosphorylation | Uniprot | |
| S144 | Phosphorylation | Uniprot | |
| S149 | Phosphorylation | Uniprot | |
| S151 | Phosphorylation | Uniprot | |
| S152 | Phosphorylation | Uniprot | |
| T155 | Phosphorylation | Uniprot | |
| S180 | Phosphorylation | Uniprot | |
| S188 | Phosphorylation | Uniprot | |
| S191 | Phosphorylation | Uniprot | |
| T195 | Phosphorylation | Uniprot | |
| S200 | Phosphorylation | Uniprot | |
| T220 | Phosphorylation | Uniprot | |
| Y222 | Phosphorylation | Uniprot | |
| K228 | Ubiquitination | Uniprot | |
| K237 | Ubiquitination | Uniprot | |
| K269 | Ubiquitination | Uniprot | |
| K361 | Acetylation | Uniprot | |
| K361 | Methylation | Uniprot | |
| K361 | Ubiquitination | Uniprot | |
| K366 | Acetylation | Uniprot | |
| K366 | Ubiquitination | Uniprot | |
| K372 | Methylation | Uniprot | |
| K372 | Ubiquitination | Uniprot | |
| Y374 | Phosphorylation | Uniprot | |
| T378 | Phosphorylation | Uniprot | |
| Y459 | Phosphorylation | Uniprot | |
| K466 | Acetylation | Uniprot | |
| K466 | Ubiquitination | Uniprot | |
| S491 | O-Glycosylation | Uniprot | |
| T494 | Phosphorylation | Uniprot | |
| K499 | Ubiquitination | Uniprot | |
| S505 | Phosphorylation | Uniprot | |
| K506 | Ubiquitination | Uniprot | |
| S510 | Phosphorylation | Uniprot | |
| S521 | Phosphorylation | Uniprot | |
| R526 | Methylation | Uniprot | |
| S540 | Phosphorylation | Uniprot | |
| S542 | Phosphorylation | Uniprot | |
| S551 | Phosphorylation | Uniprot | |
| S555 | Phosphorylation | Uniprot | |
| T559 | Phosphorylation | Uniprot | |
| S560 | Phosphorylation | Uniprot | |
| T565 | Phosphorylation | Uniprot | |
| S578 | Phosphorylation | Uniprot | |
| Y660 | Phosphorylation | P00533 (EGFR) | Uniprot |
| S664 | Phosphorylation | Uniprot | |
| S674 | Phosphorylation | Uniprot | |
| S684 | Phosphorylation | Uniprot | |
| K689 | Ubiquitination | Uniprot | |
| S709 | Phosphorylation | Uniprot | |
| T710 | Phosphorylation | Uniprot | |
| S712 | Phosphorylation | Uniprot | |
| T716 | Phosphorylation | Uniprot | |
| T736 | Phosphorylation | Uniprot | |
| K747 | Ubiquitination | Uniprot | |
| Y767 | Phosphorylation | Uniprot | |
| T786 | Phosphorylation | Uniprot | |
| T794 | Phosphorylation | Uniprot | |
| T806 | Phosphorylation | Uniprot | |
| K808 | Ubiquitination | Uniprot | |
| S812 | Phosphorylation | Uniprot | |
| T823 | Phosphorylation | Uniprot | |
| K842 | Ubiquitination | Uniprot | |
| S849 | Phosphorylation | Uniprot | |
| T851 | Phosphorylation | Uniprot | |
| T859 | Phosphorylation | Uniprot |
研究背景
Protein 4.1 is a major structural element of the erythrocyte membrane skeleton. It plays a key role in regulating membrane physical properties of mechanical stability and deformability by stabilizing spectrin-actin interaction. Recruits DLG1 to membranes. Required for dynein-dynactin complex and NUMA1 recruitment at the mitotic cell cortex during anaphase.
Phosphorylated at multiple sites by different protein kinases and each phosphorylation event selectively modulates the protein's functions.
Phosphorylation on Tyr-660 reduces the ability of 4.1 to promote the assembly of the spectrin/actin/4.1 ternary complex.
O-glycosylated; contains N-acetylglucosamine side chains in the C-terminal domain.
Cytoplasm>Cytoskeleton. Cytoplasm>Cell cortex. Nucleus.
Binds with a high affinity to glycophorin and with lower affinity to band III protein. Associates with the nuclear mitotic apparatus. Interacts with calmodulin. Interacts with CENPJ. Interacts with DLG1. Also found to associate with contractile apparatus and tight junctions. Interacts with NUMA1; this interaction is negatively regulated by CDK1 during metaphase and promotes anaphase-specific localization of NUMA1 in symmetrically dividing cells. Interacts with ATP2B1; regulates small intestinal calcium absorption through regulation of membrane expression of ATP2B1 (By similarity).
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