Phospho-Ras-GRF1 (Ser916) Antibody - #AF3948
| 製品: | Phospho-Ras-GRF1 (Ser916) Antibody |
| カタログ: | AF3948 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-Ras-GRF1 (Ser916) |
| アプリケーション: | IHC |
| 反応性: | Human, Mouse, Rat |
| 分子量: | 145kD(Calculated). |
| ユニプロット: | Q13972 |
| RRID: | AB_2847671 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF3948, RRID:AB_2847671.
折りたたみ／展開
CDC25; CDC25L; GNRP; GRF1; GRF55; Guanine nucleotide exchange factor; Guanine nucleotide releasing factor 55 kD; Guanine nucleotide releasing protein; guanine nucleotide-releasing factor 1; Guanine nucleotide-releasing protein; H GRF55; HGRF55; PP13187; Ras protein specific guanine nucleotide releasing factor 1; Ras specific guanine nucleotide releasing factor; Ras specific guanine nucleotide releasing factor CDC25 homolog; Ras specific nucleotide exchange factor CDC25; Ras-GRF1; Ras-specific guanine nucleotide-releasing factor 1; Ras-specific nucleotide exchange factor CDC25; RASGRF1; RGRF1_HUMAN;
免疫原
A synthesized peptide derived from human Ras-GRF1 around the phosphorylation site of Ser916.
- Q13972 RGRF1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MQKGIRLNDGHVASLGLLARKDGTRKGYLSKRSSDNTKWQTKWFALLQNLLFYFESDSSSRPSGLYLLEGCVCDRAPSPKPALSAKEPLEKQHYFTVNFSHENQKALELRTEDAKDCDEWVAAIAHASYRTLATEHEALMQKYLHLLQIVETEKTVAKQLRQQIEDGEIEIERLKAEITSLLKDNERIQSTQTVAPNDEDSDIKKIKKVQSFLRGWLCRRKWKTIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKHYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMIIEGCEILLDTSQTFVRQGSLIQVPMSEKGKITRGRLGSLSLKKEGERQCFLFSKHLIICTRGSGGKLHLTKNGVISLIDCTLLEEPESTEEEAKGSGQDIDHLDFKIGVEPKDSPPFTVILVASSRQEKAAWTSDISQCVDNIRCNGLMMNAFEENSKVTVPQMIKRTREGTREAEMSRSDASLYCDDVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTTAIVVLDKLITIYKKPISAIPARWLRSLELLFASGQNNKLLYGEPPKSPRATRKFSSPPPLSITKTSSPSRRRKLSLNIPIITGGKALDLAALSCNSNGYTSMYSAMSPFSKATLDTSKLYVSSSFTNKIPDEGDTTPEKPEDPSALSKQSSEVSMREESDIDQNQSDDGDTETSPTKSPTTPKSVKNKNSSEFPLFSYNNGVVMTSCRELDNNRSALSAASAFAIATAGANEGTPNKEKYRRMSLASAGFPPDQRNGDKEFVIRRAATNRVLNVLRHWVSKHSQDFETNDELKCKVIGFLEEVMHDPELLTQERKAAANIIRTLTQEDPGDNQITLEEITQMAEGVKAEPFENHSALEIAEQLTLLDHLVFKKIPYEEFFGQGWMKLEKNERTPYIMKTTKHFNDISNLIASEIIRNEDINARVSAIEKWVAVADICRCLHNYNAVLEITSSMNRSAIFRLKKTWLKVSKQTKALIDKLQKLVSSEGRFKNLREALKNCDPPCVPYLGMYLTDLAFIEEGTPNYTEDGLVNFSKMRMISHIIREIRQFQQTAYKIEHQAKVTQYLLDQSFVMDEESLYESSLRIEPKLPT
PTMs - Q13972 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S14 | Phosphorylation | Uniprot | |
| S287 | Phosphorylation | O96017 (CHEK2) | Uniprot |
| S481 | Phosphorylation | Uniprot | |
| S709 | Phosphorylation | Uniprot | |
| S730 | Phosphorylation | Uniprot | |
| S738 | Phosphorylation | Uniprot | |
| S750 | Phosphorylation | Uniprot | |
| S752 | Phosphorylation | Uniprot | |
| S758 | Phosphorylation | Uniprot | |
| S787 | Phosphorylation | Uniprot | |
| S927 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| T1023 | Phosphorylation | Uniprot | |
| S1090 | Phosphorylation | Uniprot | |
| S1095 | Phosphorylation | Uniprot | |
| S1252 | Phosphorylation | Uniprot |
研究背景
Promotes the exchange of Ras-bound GDP by GTP.
Phosphorylated by PLK2, leading to ubiquitination and degradation by the proteasome.
Ubiquitinated and degraded following phosphorylation by PLK2.
Phosphorylated by SRC and LCK. Phosphorylation by LCK increases its capacity to stimulate the GDP/GTP exchange on Ras, whereas its phosphorylation by SRC seems not to have an effect on stimulation activity.
Homooligomer and heterooligomer with RASGRF2. Interacts with USP8, thereby regulating its stability (By similarity).
The DH (DBL-homology) domain mediates interaction with RASGRF2.
研究領域
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)
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