EPHA3/4/5 Antibody - #AF6967
製品: | EPHA3/4/5 Antibody |
カタログ: | AF6967 |
タンパク質の説明: | Rabbit polyclonal antibody to EPHA3/4/5 |
アプリケーション: | ELISA(peptide) |
反応性: | Human, Mouse, Rat |
分子量: | 110kD,115kD(Calculated). |
ユニプロット: | P29320 | P54764 | P54756 |
RRID: | AB_2847757 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF6967, RRID:AB_2847757.
折りたたみ/展開
AW492086; Cek4; EC 2.7.10.1; EK4; End3; Eph receptor A3; EPH-like kinase 4; EPH-like tyrosine kinase 1; EPHA3; EPHA3_HUMAN; Ephrin receptor EphA3; Ephrin type-A receptor 3; ETK 1; ETK; ETK1; HEK 4; HEK; HEK4; Human embryo kinase 1; Human embryo kinase; Mek4; MGC109882; Receptor tyrosine kinase HEK; Testicular tissue protein Li 64; Tyro 4; Tyro4; TYRO4 protein tyrosine kinase; Tyrosine protein kinase receptor ETK 1; Tyrosine-protein kinase receptor ETK1; Tyrosine-protein kinase TYRO4;
免疫原
A synthesized peptide derived from human EPHA3/4/5.
Widely expressed. Highest level in placenta.
P54764 EPHA4_HUMAN:Ubiquitous.
P54756 EPHA5_HUMAN:Almost exclusively expressed in the nervous system in cortical neurons, cerebellar Purkinje cells and pyramidal neurons within the cortex and hippocampus. Display an increasing gradient of expression from the forebrain to hindbrain and spinal cord.
- P29320 EPHA3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDCQLSILLLLSCSVLDSFGELIPQPSNEVNLLDSKTIQGELGWISYPSHGWEEISGVDEHYTPIRTYQVCNVMDHSQNNWLRTNWVPRNSAQKIYVELKFTLRDCNSIPLVLGTCKETFNLYYMESDDDHGVKFREHQFTKIDTIAADESFTQMDLGDRILKLNTEIREVGPVNKKGFYLAFQDVGACVALVSVRVYFKKCPFTVKNLAMFPDTVPMDSQSLVEVRGSCVNNSKEEDPPRMYCSTEGEWLVPIGKCSCNAGYEERGFMCQACRPGFYKALDGNMKCAKCPPHSSTQEDGSMNCRCENNYFRADKDPPSMACTRPPSSPRNVISNINETSVILDWSWPLDTGGRKDVTFNIICKKCGWNIKQCEPCSPNVRFLPRQFGLTNTTVTVTDLLAHTNYTFEIDAVNGVSELSSPPRQFAAVSITTNQAAPSPVLTIKKDRTSRNSISLSWQEPEHPNGIILDYEVKYYEKQEQETSYTILRARGTNVTISSLKPDTIYVFQIRARTAAGYGTNSRKFEFETSPDSFSISGESSQVVMIAISAAVAIILLTVVIYVLIGRFCGYKSKHGADEKRLHFGNGHLKLPGLRTYVDPHTYEDPTQAVHEFAKELDATNISIDKVVGAGEFGEVCSGRLKLPSKKEISVAIKTLKVGYTEKQRRDFLGEASIMGQFDHPNIIRLEGVVTKSKPVMIVTEYMENGSLDSFLRKHDAQFTVIQLVGMLRGIASGMKYLSDMGYVHRDLAARNILINSNLVCKVSDFGLSRVLEDDPEAAYTTRGGKIPIRWTSPEAIAYRKFTSASDVWSYGIVLWEVMSYGERPYWEMSNQDVIKAVDEGYRLPPPMDCPAALYQLMLDCWQKDRNNRPKFEQIVSILDKLIRNPGSLKIITSAAARPSNLLLDQSNVDITTFRTTGDWLNGVWTAHCKEIFTGVEYSSCDTIAKISTDDMKKVGVTVVGPQKKIISSIKALETQSKNGPVPV
- P54764 EPHA4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGIFYFALFSCLFGICDAVTGSRVYPANEVTLLDSRSVQGELGWIASPLEGGWEEVSIMDEKNTPIRTYQVCNVMEPSQNNWLRTDWITREGAQRVYIEIKFTLRDCNSLPGVMGTCKETFNLYYYESDNDKERFIRENQFVKIDTIAADESFTQVDIGDRIMKLNTEIRDVGPLSKKGFYLAFQDVGACIALVSVRVFYKKCPLTVRNLAQFPDTITGADTSSLVEVRGSCVNNSEEKDVPKMYCGADGEWLVPIGNCLCNAGHEERSGECQACKIGYYKALSTDATCAKCPPHSYSVWEGATSCTCDRGFFRADNDAASMPCTRPPSAPLNLISNVNETSVNLEWSSPQNTGGRQDISYNVVCKKCGAGDPSKCRPCGSGVHYTPQQNGLKTTKVSITDLLAHTNYTFEIWAVNGVSKYNPNPDQSVSVTVTTNQAAPSSIALVQAKEVTRYSVALAWLEPDRPNGVILEYEVKYYEKDQNERSYRIVRTAARNTDIKGLNPLTSYVFHVRARTAAGYGDFSEPLEVTTNTVPSRIIGDGANSTVLLVSVSGSVVLVVILIAAFVISRRRSKYSKAKQEADEEKHLNQGVRTYVDPFTYEDPNQAVREFAKEIDASCIKIEKVIGVGEFGEVCSGRLKVPGKREICVAIKTLKAGYTDKQRRDFLSEASIMGQFDHPNIIHLEGVVTKCKPVMIITEYMENGSLDAFLRKNDGRFTVIQLVGMLRGIGSGMKYLSDMSYVHRDLAARNILVNSNLVCKVSDFGMSRVLEDDPEAAYTTRGGKIPIRWTAPEAIAYRKFTSASDVWSYGIVMWEVMSYGERPYWDMSNQDVIKAIEEGYRLPPPMDCPIALHQLMLDCWQKERSDRPKFGQIVNMLDKLIRNPNSLKRTGTESSRPNTALLDPSSPEFSAVVSVGDWLQAIKMDRYKDNFTAAGYTTLEAVVHVNQEDLARIGITAITHQNKILSSVQAMRTQMQQMHGRMVPV
- P54756 EPHA5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRGSGPRGAGRRRPPSGGGDTPITPASLAGCYSAPRRAPLWTCLLLCAALRTLLASPSNEVNLLDSRTVMGDLGWIAFPKNGWEEIGEVDENYAPIHTYQVCKVMEQNQNNWLLTSWISNEGASRIFIELKFTLRDCNSLPGGLGTCKETFNMYYFESDDQNGRNIKENQYIKIDTIAADESFTELDLGDRVMKLNTEVRDVGPLSKKGFYLAFQDVGACIALVSVRVYYKKCPSVVRHLAVFPDTITGADSSQLLEVSGSCVNHSVTDEPPKMHCSAEGEWLVPIGKCMCKAGYEEKNGTCQVCRPGFFKASPHIQSCGKCPPHSYTHEEASTSCVCEKDYFRRESDPPTMACTRPPSAPRNAISNVNETSVFLEWIPPADTGGRKDVSYYIACKKCNSHAGVCEECGGHVRYLPRQSGLKNTSVMMVDLLAHTNYTFEIEAVNGVSDLSPGARQYVSVNVTTNQAAPSPVTNVKKGKIAKNSISLSWQEPDRPNGIILEYEIKYFEKDQETSYTIIKSKETTITAEGLKPASVYVFQIRARTAAGYGVFSRRFEFETTPVFAASSDQSQIPVIAVSVTVGVILLAVVIGVLLSGSCCECGCGRASSLCAVAHPSLIWRCGYSKAKQDPEEEKMHFHNGHIKLPGVRTYIDPHTYEDPNQAVHEFAKEIEASCITIERVIGAGEFGEVCSGRLKLPGKRELPVAIKTLKVGYTEKQRRDFLGEASIMGQFDHPNIIHLEGVVTKSKPVMIVTEYMENGSLDTFLKKNDGQFTVIQLVGMLRGISAGMKYLSDMGYVHRDLAARNILINSNLVCKVSDFGLSRVLEDDPEAAYTTRGGKIPIRWTAPEAIAFRKFTSASDVWSYGIVMWEVVSYGERPYWEMTNQDVIKAVEEGYRLPSPMDCPAALYQLMLDCWQKERNSRPKFDEIVNMLDKLIRNPSSLKTLVNASCRVSNLLAEHSPLGSGAYRSVGEWLEAIKMGRYTEIFMENGYSSMDAVAQVTLEDLRRLGVTLVGHQKKIMNSLQEMKVQLVNGMVPL
PTMs - P29320/P54764/P54756 基板として
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S294 | Phosphorylation | Uniprot | |
T432 | Phosphorylation | Uniprot | |
T442 | Phosphorylation | Uniprot | |
T485 | Phosphorylation | Uniprot | |
S497 | Phosphorylation | Uniprot | |
S498 | Phosphorylation | Uniprot | |
Y561 | Phosphorylation | Uniprot | |
Y570 | Phosphorylation | Uniprot | |
T595 | Phosphorylation | Uniprot | |
Y596 | Phosphorylation | P29320 (EPHA3) | Uniprot |
T601 | Phosphorylation | Uniprot | |
Y602 | Phosphorylation | P29320 (EPHA3) | Uniprot |
K625 | Ubiquitination | Uniprot | |
T654 | Phosphorylation | Uniprot | |
K656 | Ubiquitination | Uniprot | |
Y659 | Phosphorylation | Uniprot | |
Y701 | Phosphorylation | P29320 (EPHA3) | Uniprot |
Y736 | Phosphorylation | Uniprot | |
Y742 | Phosphorylation | Uniprot | |
S768 | Phosphorylation | Uniprot | |
Y779 | Phosphorylation | P29320 (EPHA3) | Uniprot |
T781 | Phosphorylation | Uniprot | |
Y937 | Phosphorylation | Uniprot | |
T974 | Phosphorylation | Uniprot | |
S976 | Phosphorylation | Uniprot |
Site | PTM Type | Enzyme | Source |
---|---|---|---|
T117 | Phosphorylation | Uniprot | |
T168 | Phosphorylation | Uniprot | |
S349 | Phosphorylation | Uniprot | |
S350 | Phosphorylation | Uniprot | |
T354 | Phosphorylation | Uniprot | |
T595 | Phosphorylation | Uniprot | |
Y596 | Phosphorylation | P54764 (EPHA4) | Uniprot |
T601 | Phosphorylation | Uniprot | |
Y602 | Phosphorylation | P54764 (EPHA4) | Uniprot |
S637 | Phosphorylation | Uniprot | |
K693 | Acetylation | Uniprot | |
K735 | Ubiquitination | Uniprot | |
S741 | Phosphorylation | Uniprot | |
Y779 | Phosphorylation | Uniprot | |
T781 | Phosphorylation | Uniprot | |
Y798 | Phosphorylation | Uniprot | |
S887 | Phosphorylation | Uniprot | |
T957 | Phosphorylation | Uniprot |
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S4 | Phosphorylation | Uniprot | |
T197 | Phosphorylation | Uniprot | |
Y295 | Phosphorylation | Uniprot | |
T301 | Phosphorylation | Uniprot | |
S313 | Phosphorylation | Uniprot | |
S318 | Phosphorylation | Uniprot | |
K387 | Ubiquitination | Uniprot | |
T649 | Phosphorylation | Uniprot | |
Y650 | Phosphorylation | Uniprot | |
T655 | Phosphorylation | Uniprot | |
Y656 | Phosphorylation | Uniprot | |
S673 | Phosphorylation | Uniprot | |
T676 | Phosphorylation | Uniprot | |
K699 | Ubiquitination | Uniprot | |
T708 | Phosphorylation | Uniprot | |
K710 | Ubiquitination | Uniprot | |
Y713 | Phosphorylation | Uniprot | |
Y790 | Phosphorylation | Uniprot | |
Y796 | Phosphorylation | Uniprot | |
S822 | Phosphorylation | Uniprot | |
Y833 | Phosphorylation | Uniprot | |
T835 | Phosphorylation | Uniprot | |
T856 | Phosphorylation | Uniprot | |
S873 | Phosphorylation | Uniprot | |
S953 | Phosphorylation | Uniprot | |
S960 | Phosphorylation | Uniprot | |
S964 | Phosphorylation | Uniprot | |
Y967 | Phosphorylation | Uniprot |
PTMs - P29320/P54764/P54756 酵素として
Substrate | Site | Source |
---|---|---|
P29320 (EPHA3) | Y596 | Uniprot |
P29320 (EPHA3) | Y602 | Uniprot |
P29320 (EPHA3) | Y701 | Uniprot |
P29320 (EPHA3) | Y779 | Uniprot |
P40763-1 (STAT3) | Y705 | Uniprot |
Substrate | Site | Source |
---|---|---|
P19174 (PLCG1) | Y783 | Uniprot |
P54764-1 (EPHA4) | Y596 | Uniprot |
P54764 (EPHA4) | Y602 | Uniprot |
Q00535 (CDK5) | Y15 | Uniprot |
Substrate | Site | Source |
---|
研究背景
Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous for ephrin-A ligands it binds preferentially EFNA5. Upon activation by EFNA5 regulates cell-cell adhesion, cytoskeletal organization and cell migration. Plays a role in cardiac cells migration and differentiation and regulates the formation of the atrioventricular canal and septum during development probably through activation by EFNA1. Involved in the retinotectal mapping of neurons. May also control the segregation but not the guidance of motor and sensory axons during neuromuscular circuit development.
Autophosphorylates upon activation by EFNA5. Phosphorylation on Tyr-602 mediates interaction with NCK1. Dephosphorylated by PTPN1.
Cell membrane>Single-pass type I membrane protein.
Secreted.
Widely expressed. Highest level in placenta.
Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses. Forms a ternary EFNA5-EPHA3-ADAM10 complex mediating EFNA5 extracellular domain shedding by ADAM10 which regulates the EFNA5-EPHA3 complex internalization and function. Interacts with NCK1 (via SH2 domain); mediates EFNA5-EPHA3 signaling (By similarity). Interacts (phosphorylated) with PTPN1; dephosphorylates EPHA3 and may regulate its trafficking and function. Interacts (phosphorylated) with CRK; mediates EFNA5-EPHA3 signaling through RHOA GTPase activation.
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Receptor tyrosine kinase which binds membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous, it has the unique property among Eph receptors to bind and to be physiologically activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands including EFNA1 and EFNB3. Upon activation by ephrin ligands, modulates cell morphology and integrin-dependent cell adhesion through regulation of the Rac, Rap and Rho GTPases activity. Plays an important role in the development of the nervous system controlling different steps of axonal guidance including the establishment of the corticospinal projections. May also control the segregation of motor and sensory axons during neuromuscular circuit development. In addition to its role in axonal guidance plays a role in synaptic plasticity. Activated by EFNA1 phosphorylates CDK5 at 'Tyr-15' which in turn phosphorylates NGEF regulating RHOA and dendritic spine morphogenesis. In the nervous system, plays also a role in repair after injury preventing axonal regeneration and in angiogenesis playing a role in central nervous system vascular formation. Additionally, its promiscuity makes it available to participate in a variety of cell-cell signaling regulating for instance the development of the thymic epithelium. During development of the cochlear organ of Corti, regulates pillar cell separation by forming a ternary complex with ADAM10 and CADH1 which facilitates the cleavage of CADH1 by ADAM10 and disruption of adherens junctions (By similarity).
Cell membrane>Single-pass type I membrane protein. Cell projection>Axon. Cell projection>Dendrite. Cell junction>Synapse>Postsynaptic density membrane. Early endosome. Cell junction>Adherens junction.
Note: Clustered upon activation and targeted to early endosome.
Ubiquitous.
Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses. Interacts (phosphorylated at position Tyr-602) with FYN. Interacts with CDK5, CDK5R1 and NGEF; upon activation by EFNA1 induces NGEF phosphorylation by the kinase CDK5. Interacts with CHN1; effector of EPHA4 in axon guidance linking EPHA4 activation to RAC1 regulation (By similarity). Interacts (via PDZ motif) with SIPA1L1 (via PDZ domain); controls neuronal morphology through regulation of the RAP1 (RAP1A or RAP1B) and RAP2 (RAP2A, RAP2B or RAP2C) GTPases. Forms a ternary complex composed of ADAM10, CADH1 and EPHA4; within the complex, CADH1 is cleaved by ADAM10 which disrupts adherens junctions (By similarity).
The protein kinase domain mediates interaction with NGEF.
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 most probably constitutes the cognate/functional ligand for EPHA5. Functions as an axon guidance molecule during development and may be involved in the development of the retinotectal, entorhino-hippocampal and hippocamposeptal pathways. Together with EFNA5 plays also a role in synaptic plasticity in adult brain through regulation of synaptogenesis. In addition to its function in the nervous system, the interaction of EPHA5 with EFNA5 mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion (By similarity).
Phosphorylated. Phosphorylation is stimulated by the ligand EFNA5. Dephosphorylation upon stimulation by glucose, inhibits EPHA5 forward signaling and results in insulin secretion (By similarity).
Cell membrane>Single-pass type I membrane protein. Cell projection>Axon. Cell projection>Dendrite.
Almost exclusively expressed in the nervous system in cortical neurons, cerebellar Purkinje cells and pyramidal neurons within the cortex and hippocampus. Display an increasing gradient of expression from the forebrain to hindbrain and spinal cord.
Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses (By similarity). Interacts (via SAM domain) with SAMD5 (via SAM domain) (By similarity).
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
研究領域
· Organismal Systems > Development > Axon guidance. (View pathway)
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