製品: PAK2 Antibody
カタログ: AF2681
タンパク質の説明: Rabbit polyclonal antibody to PAK2
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 61-67kDa; 58kD(Calculated).
ユニプロット: Q13177
RRID: AB_2847783

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
PAK2 Antibody detects endogenous levels of PAK2.
RRID:
AB_2847783
引用形式: Affinity Biosciences Cat# AF2681, RRID:AB_2847783.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

C-t-PAK2; CB422; EC 2.7.11.1; Gamma PAK; Gamma-PAK; hPAK65; Kinase; p21 (CDKN1A) activated kinase 2; p21 (CDKN1A)-activated kinase 2a; p21 activated kinase 2; p21 protein (Cdc42/Rac)-activated kinase 2; p21 protein Cdc42 Rac activated kinase 2; p21-activated kinase 2; p21-activated kinase, 65-KD; p21-activated protein kinase I; p21CDKN1A activated kinase 2; p27; p34; p58; p65PAK; PAK 2; PAK-2; PAK-2p34; Pak2; PAK2_HUMAN; PAK65; PAKgamma; S6 H4 kinase; S6/H4 kinase; Serine threonine protein kinase PAK 2; Serine/threonine protein kinase PAK 2;

免疫原

免疫原:

A synthesized peptide derived from human PAK2.

Uniprot:
遺伝子(ID):
発現特異性:
Q13177 PAK2_HUMAN:

Ubiquitously expressed. Higher levels seen in skeletal muscle, ovary, thymus and spleen.

タンパク質配列:
MSDNGELEDKPPAPPVRMSSTIFSTGGKDPLSANHSLKPLPSVPEEKKPRHKIISIFSGTEKGSKKKEKERPEISPPSDFEHTIHVGFDAVTGEFTGMPEQWARLLQTSNITKLEQKKNPQAVLDVLKFYDSNTVKQKYLSFTPPEKDGFPSGTPALNAKGTEAPAVVTEEEDDDEETAPPVIAPRPDHTKSIYTRSVIDPVPAPVGDSHVDGAAKSLDKQKKKTKMTDEEIMEKLRTIVSIGDPKKKYTRYEKIGQGASGTVFTATDVALGQEVAIKQINLQKQPKKELIINEILVMKELKNPNIVNFLDSYLVGDELFVVMEYLAGGSLTDVVTETCMDEAQIAAVCRECLQALEFLHANQVIHRDIKSDNVLLGMEGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPELQNPEKLSPIFRDFLNRCLEMDVEKRGSAKELLQHPFLKLAKPLSSLTPLIMAAKEAMKSNR

PTMs - Q13177 基板として

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
S19 Phosphorylation Q13177 (PAK2)
S20 Phosphorylation P54646 (PRKAA2) , Q13177 (PAK2)
T21 Phosphorylation
S24 Phosphorylation
T25 Phosphorylation
K28 Acetylation
K28 Ubiquitination
S32 Phosphorylation
S36 Phosphorylation
K38 Acetylation
K38 Ubiquitination
S42 Phosphorylation
K52 Ubiquitination
S55 Phosphorylation Q13177 (PAK2)
S58 Phosphorylation
T60 Phosphorylation
K62 Acetylation
K62 Ubiquitination
S64 Phosphorylation
S75 Phosphorylation
T112 Phosphorylation
K113 Ubiquitination
K128 Acetylation
K128 Ubiquitination
Y130 Phosphorylation P12931 (SRC)
S132 Phosphorylation
T134 Phosphorylation
K136 Ubiquitination
Y139 Phosphorylation
S141 Phosphorylation Q13177 (PAK2)
T143 Phosphorylation
K147 Ubiquitination
S152 Phosphorylation
T154 Phosphorylation
T169 Phosphorylation
K191 Ubiquitination
S192 Phosphorylation Q13177 (PAK2)
Y194 Phosphorylation
T195 Phosphorylation
S197 Phosphorylation Q13177 (PAK2)
G213 Myristoylation
K216 Ubiquitination
S217 Phosphorylation
K226 Ubiquitination
T228 Phosphorylation
K235 Ubiquitination
K254 Ubiquitination
K278 Ubiquitination
K284 Ubiquitination
K299 Ubiquitination
K302 Ubiquitination
K370 Ubiquitination
S381 Phosphorylation
T394 Phosphorylation
K399 Acetylation
K399 Ubiquitination
S401 Phosphorylation
T402 Phosphorylation Q13177 (PAK2)
T406 Phosphorylation
Y443 Phosphorylation
Y453 Phosphorylation
K468 Ubiquitination
K487 Ubiquitination
S490 Phosphorylation
K492 Acetylation
K492 Ubiquitination
K501 Acetylation
K501 Ubiquitination
K504 Ubiquitination
S507 Phosphorylation
S522 Phosphorylation

PTMs - Q13177 酵素として

Substrate Site Source
P00519 (ABL1) S618 Uniprot
P00519 (ABL1) S619 Uniprot
P01106 (MYC) T358 Uniprot
P01106 (MYC) S373 Uniprot
P01106 (MYC) T400 Uniprot
P01236 (PRL) S179 Uniprot
P01236 (PRL) S207 Uniprot
P05412 (JUN) T2 Uniprot
P05412 (JUN) T8 Uniprot
P05412 (JUN) T89 Uniprot
P05412 (JUN) T93 Uniprot
P05412 (JUN) T286 Uniprot
P08670 (VIM) S26 Uniprot
P08670 (VIM) S39 Uniprot
P08670 (VIM) S51 Uniprot
P08670 (VIM) S66 Uniprot
P08670 (VIM) S73 Uniprot
P17600-2 (SYN1) S9 Uniprot
P19105 (MYL12A) S19 Uniprot
P24844 (MYL9) S20 Uniprot
P31152 (MAPK4) S186 Uniprot
P35240 (NF2) S518 Uniprot
P49023 (PXN) S272 Uniprot
P49023 (PXN) S274 Uniprot
P55210 (CASP7) S30 Uniprot
P55210 (CASP7) T173 Uniprot
P55210 (CASP7) S239 Uniprot
P62753 (RPS6) S235 Uniprot
P62753 (RPS6) S236 Uniprot
P62753 (RPS6) S240 Uniprot
P62753 (RPS6) S242 Uniprot
P62805 (HIST4H4) S48 Uniprot
Q04637 (EIF4G1) S895 Uniprot
Q05682 (CALD1) S714 Uniprot
Q05682 (CALD1) S744 Uniprot
Q13177 (PAK2) S19 Uniprot
Q13177 (PAK2) S20 Uniprot
Q13177 (PAK2) S55 Uniprot
Q13177 (PAK2) S141 Uniprot
Q13177 (PAK2) S192 Uniprot
Q13177 (PAK2) S197 Uniprot
Q13177 (PAK2) T402 Uniprot
Q15746 (MYLK) S1208 Uniprot
Q15746 (MYLK) S1759 Uniprot
Q15746-1 (MYLK) S1760 Uniprot
Q15796 (SMAD2) S417 Uniprot
Q16659 (MAPK6) S189 Uniprot
Q70Z35 (PREX2) S1107 Uniprot
Q9BUB5 (MKNK1) S39 Uniprot

研究背景

機能:

Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell motility, cell cycle progression, apoptosis or proliferation. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Full-length PAK2 stimulates cell survival and cell growth. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Phosphorylates JUN and plays an important role in EGF-induced cell proliferation. Phosphorylates many other substrates including histone H4 to promote assembly of H3.3 and H4 into nucleosomes, BAD, ribosomal protein S6, or MBP. Additionally, associates with ARHGEF7 and GIT1 to perform kinase-independent functions such as spindle orientation control during mitosis. On the other hand, apoptotic stimuli such as DNA damage lead to caspase-mediated cleavage of PAK2, generating PAK-2p34, an active p34 fragment that translocates to the nucleus and promotes cellular apoptosis involving the JNK signaling pathway. Caspase-activated PAK2 phosphorylates MKNK1 and reduces cellular translation.

PTMs:

Full-length PAK2 is autophosphorylated when activated by CDC42/p21. Following cleavage, both peptides, PAK-2p27 and PAK-2p34, become highly autophosphorylated, with PAK-2p27 being phosphorylated on serine and PAK-2p34 on threonine residues, respectively. Autophosphorylation of PAK-2p27 can occur in the absence of any effectors and is dependent on phosphorylation of Thr-402, because PAK-2p27 is acting as an exogenous substrate.

During apoptosis proteolytically cleaved by caspase-3 or caspase-3-like proteases to yield active PAK-2p34.

Ubiquitinated, leading to its proteasomal degradation.

PAK-2p34 is myristoylated.

細胞の位置付け:

Cytoplasm.
Note: MYO18A mediates the cellular distribution of the PAK2-ARHGEF7-GIT1 complex to the inner surface of the cell membrane.

Nucleus. Cytoplasm>Perinuclear region. Membrane>Lipid-anchor.
Note: Interaction with ARHGAP10 probably changes PAK-2p34 location to cytoplasmic perinuclear region. Myristoylation changes PAK-2p34 location to the membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Ubiquitously expressed. Higher levels seen in skeletal muscle, ovary, thymus and spleen.

サブユニット構造:

Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and RAC1. Interacts with SH3MD4. Interacts with SCRIB. Interacts with ARHGEF7 and GIT1. PAK-2p34 interacts with ARHGAP10. Interacts with RAC1.

(Microbial infection) Interacts with and activated by HIV-1 Nef.

タンパク質ファミリー:

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

研究領域

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Human Diseases > Cancers: Specific types > Renal cell carcinoma.   (View pathway)

· Organismal Systems > Development > Axon guidance.   (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway.   (View pathway)

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