製品: PTP alpha Antibody
カタログ: AF2692
タンパク質の説明: Rabbit polyclonal antibody to PTP alpha
アプリケーション: WB
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 90kDa; 91kD(Calculated).
ユニプロット: P18433
RRID: AB_2847787

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
PTP Antibody detects endogenous levels of PTP .
RRID:
AB_2847787
引用形式: Affinity Biosciences Cat# AF2692, RRID:AB_2847787.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

HEPTP; HLPR; HPTP alpha; HPTPA; HPTPalpha; Leukocyte common antigen related peptide; LRP; Protein tyrosine phosphatase alpha; Protein tyrosine phosphatase receptor type A; Protein tyrosine phosphatase receptor type alpha polypeptide; Protein tyrosine phosphate; Protein-tyrosine phosphatase alpha; PTPA; PTPalpha; PTPase alpha; PTPLCA related phosphatase; Ptpra; PTPRA_HUMAN; PTPRL 2; PTPRL2; PTRA; R PTP alpha; R-PTP-alpha; Receptor type tyrosine protein phosphatase alpha; Receptor-type tyrosine-protein phosphatase alpha; RPTPA; Tyrosine phosphatase alpha;

免疫原

免疫原:

A synthesized peptide derived from human PTP alpha , corresponding to a region within the internal amino acids.

Uniprot:
遺伝子(ID):
タンパク質配列:
MDSWFILVLLGSGLICVSANNATTVAPSVGITRLINSSTAEPVKEEAKTSNPTSSLTSLSVAPTFSPNITLGPTYLTTVNSSDSDNGTTRTASTNSIGITISPNGTWLPDNQFTDARTEPWEGNSSTAATTPETFPPSGNSDSKDRRDETPIIAVMVALSSLLVIVFIIIVLYMLRFKKYKQAGSHSNSFRLSNGRTEDVEPQSVPLLARSPSTNRKYPPLPVDKLEEEINRRMADDNKLFREEFNALPACPIQATCEAASKEENKEKNRYVNILPYDHSRVHLTPVEGVPDSDYINASFINGYQEKNKFIAAQGPKEETVNDFWRMIWEQNTATIVMVTNLKERKECKCAQYWPDQGCWTYGNIRVSVEDVTVLVDYTVRKFCIQQVGDMTNRKPQRLITQFHFTSWPDFGVPFTPIGMLKFLKKVKACNPQYAGAIVVHCSAGVGRTGTFVVIDAMLDMMHTERKVDVYGFVSRIRAQRCQMVQTDMQYVFIYQALLEHYLYGDTELEVTSLETHLQKIYNKIPGTSNNGLEEEFKKLTSIKIQNDKMRTGNLPANMKKNRVLQIIPYEFNRVIIPVKRGEENTDYVNASFIDGYRQKDSYIASQGPLLHTIEDFWRMIWEWKSCSIVMLTELEERGQEKCAQYWPSDGLVSYGDITVELKKEEECESYTVRDLLVTNTRENKSRQIRQFHFHGWPEVGIPSDGKGMISIIAAVQKQQQQSGNHPITVHCSAGAGRTGTFCALSTVLERVKAEGILDVFQTVKSLRLQRPHMVQTLEQYEFCYKVVQEYIDAFSDYANFK

PTMs - P18433 基板として

Site PTM Type Enzyme
S18 Phosphorylation
S187 Phosphorylation
S189 Phosphorylation Q05655 (PRKCD)
S204 Phosphorylation Q9UQM7 (CAMK2A) , Q05655 (PRKCD)
S211 Phosphorylation
S213 Phosphorylation Q05655 (PRKCD)
T214 Phosphorylation
Y218 Phosphorylation
K225 Ubiquitination
Y271 Phosphorylation
S293 Phosphorylation
K307 Ubiquitination
S407 Phosphorylation
Y522 Phosphorylation
K524 Ubiquitination
S542 Phosphorylation
T552 Phosphorylation
K560 Ubiquitination
Y588 Phosphorylation
K765 Acetylation
S766 Phosphorylation
Y791 Phosphorylation
S796 Phosphorylation
Y798 Phosphorylation P12931 (SRC)

研究背景

機能:

Tyrosine protein phosphatase which is involved in integrin-mediated focal adhesion formation (By similarity). Following integrin engagement, specifically recruits BCAR3, BCAR1 and CRK to focal adhesions thereby promoting SRC-mediated phosphorylation of BRAC1 and the subsequent activation of PAK and small GTPase RAC1 and CDC42 (By similarity).

PTMs:

Integrin binding to extracellular matrix induces phosphorylation at Tyr-798 which induces PTPRA localization and recruitment of BCAR3, BCAR1 and CRK to focal adhesions.

細胞の位置付け:

Cell membrane>Single-pass type I membrane protein. Cell junction>Focal adhesion.
Note: Localizes to focal adhesion sites following integrin engagement.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Part of a complex comprised of PTPRA, BCAR1, BCAR3 (via SH2 domain), and SRC. Within the complex, interacts (when phosphorylated on Tyr-798) with BCAR3 (via SH2 domain). Interacts with GRB2.

タンパク質ファミリー:

Belongs to the protein-tyrosine phosphatase family. Receptor class 4 subfamily.

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