IL23 Antibody - #DF13760

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製品: IL23 Antibody
カタログ: DF13760
タンパク質の説明: Rabbit polyclonal antibody to IL23
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
分子量: 23kD; 21kD(Calculated).
ユニプロット: Q9NPF7

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MS Report
HPLC Report
MSDS
説明書
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WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
IL23 Antibody detects endogenous levels of total IL23.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

免疫原

免疫原:

A synthesized peptide derived from Human IL23.

Uniprot:

Q9NPF7(IL23A_HUMAN) >>Visit HPA database.

遺伝子(ID):
IL23A(51561)
発現特異性:
Q9NPF7 IL23A_HUMAN:

Secreted by activated dendritic and phagocytic cells and keratinocytes. Also expressed by dermal Langerhans cells (at protein level).

タンパク質配列:
MLGSRAVMLLLLLPWTAQGRAVPGGSSPAWTQCQQLSQKLCTLAWSAHPLVGHMDLREEGDEETTNDVPHIQCGDGCDPQGLRDNSQFCLQRIHQGLIFYEKLLGSDIFTGEPSLLPDSPVGQLHASLLGLSQLLQPEGHHWETQQIPSLSPSQPWQRLLLRFKILRSLQAFVAVAARVFAHGAATLSP

研究背景

機能:

Associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.

細胞の位置付け:

Secreted.
Note: Secreted upon association with IL12B.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Secreted by activated dendritic and phagocytic cells and keratinocytes. Also expressed by dermal Langerhans cells (at protein level).

サブユニット構造:

Heterodimer with IL12B; disulfide-linked. The heterodimer is known as interleukin IL-23.

タンパク質ファミリー:

Belongs to the IL-6 superfamily.

研究領域

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

参考文献

1). Traditional Chinese Medicine Shi-Bi-Man ameliorates psoriasis via inhibiting IL-23/Th17 axis and CXCL16-mediated endothelial activation. Chinese medicine, 2024 (PubMed: 38429819) [IF=4.9]

Application: IF/ICC    Species: Human    Sample: T cells

Fig. 5 SBM inhibits the IL-23/Th17 axis. A Volcano plot for T cells, genes in red represented genes upregulated by IMQ, and genes in blue were downregulated by SBM. B Dotplot for the expression of genes related to IL-23/Th17 axis in T cells and keratinocytes. C Immunofluorescence images staining for CD3E (green), E-cadherin (green), IL-23 (red), IL-17A (red), and DAPI (blue) of skin tissue, scale bar = 20 μm
2). Histological Study on the Possible Ameliorating Consequence of Blocking STAT-3 Pathway on Psoriasis Model in Adult Male Albino rat. Egyptian Journal of Medical Research, 2023

Application: WB    Species: Rat    Sample:

Fig. 4: Photomicrographs of IL-23 immunostained sections in the skin [anti IL-23 immunohistochemical stain, x400]: 4a (the control group, group I): Scarce positive cytoplasmic immunoreaction (arrows) is noted in keratinocytes and dermal mononuclear cells. 4b (IMQ group, group II): Widely distributed positive cytoplasmic immunoreaction (arrows) in the keratinocytes and the dermal inflammatory cell infiltrate is seen. 4c (IMQ/STA-21 group, group III): Positive immunoreaction (arrows) can be seen confined to few keratinocytes and few mononuclear inflammatory cells in dermis. 4d: Showing the mean area percent of IL-23 positive immunoreaction.

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