HAT Antibody - #AF0269
(1)
| 製品: | HAT Antibody |
| カタログ: | AF0269 |
| タンパク質の説明: | Rabbit polyclonal antibody to HAT |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 49kDa; 50kD(Calculated). |
| ユニプロット: | O14929 |
| RRID: | AB_2833443 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF0269, RRID:AB_2833443.
折りたたみ／展開
HAT 1; hat1; HAT1_HUMAN; Histidine aminotransferase 1; Histone acetyltransferase 1; Histone acetyltransferase 1; Histone acetyltransferase type B catalytic subunit; KAT1;
免疫原
- O14929 HAT1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGFGAMEKFLVEYKSAVEKKLAEYKCNTNTAIELKLVRFPEDLENDIRTFFPEYTHQLFGDDETAFGYKGLKILLYYIAGSLSTMFRVEYASKVDENFDCVEADDVEGKIRQIIPPGFCTNTNDFLSLLEKEVDFKPFGTLLHTYSVLSPTGGENFTFQIYKADMTCRGFREYHERLQTFLMWFIETASFIDVDDERWHYFLVFEKYNKDGATLFATVGYMTVYNYYVYPDKTRPRVSQMLILTPFQGQGHGAQLLETVHRYYTEFPTVLDITAEDPSKSYVKLRDFVLVKLCQDLPCFSREKLMQGFNEDMAIEAQQKFKINKQHARRVYEILRLLVTDMSDAEQYRSYRLDIKRRLISPYKKKQRDLAKMRKCLRPEELTNQMNQIEISMQHEQLEESFQELVEDYRRVIERLAQE
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O14929 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| K9 | Ubiquitination | Uniprot | |
| K15 | Acetylation | Uniprot | |
| K15 | Ubiquitination | Uniprot | |
| K26 | Ubiquitination | Uniprot | |
| K36 | Ubiquitination | Uniprot | |
| T50 | Phosphorylation | Uniprot | |
| Y55 | Phosphorylation | Uniprot | |
| Y69 | Phosphorylation | Uniprot | |
| K70 | Ubiquitination | Uniprot | |
| Y78 | Phosphorylation | Uniprot | |
| S93 | Phosphorylation | Uniprot | |
| C101 | S-Nitrosylation | Uniprot | |
| K110 | Ubiquitination | Uniprot | |
| S190 | Phosphorylation | Uniprot | |
| T245 | Phosphorylation | Uniprot | |
| K280 | Acetylation | Uniprot | |
| K280 | Ubiquitination | Uniprot | |
| R286 | Methylation | Uniprot | |
| K304 | Ubiquitination | Uniprot | |
| K320 | Ubiquitination | Uniprot | |
| S343 | Phosphorylation | Uniprot | |
| S350 | Phosphorylation | Uniprot | |
| Y351 | Phosphorylation | Uniprot | |
| S361 | Phosphorylation | Uniprot | |
| Y363 | Phosphorylation | Uniprot |
研究背景
Acetylates soluble but not nucleosomal histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, acetylates histone H2A at 'Lys-5' (H2AK5ac). Has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG. May be involved in nucleosome assembly during DNA replication and repair as part of the histone H3.1 and H3.3 complexes. May play a role in DNA repair in response to free radical damage.
Nucleus matrix.
Cytoplasm. Nucleus. Nucleus matrix. Nucleus>Nucleoplasm.
Note: Localization is predominantly nuclear in normal cells. Treatment with hydrogen peroxide or ionizing radiation enhances nuclear localization through redistribution of existing protein.
Catalytic subunit of the type B histone acetyltransferase (HAT) complex, composed of RBBP7 and HAT1. Interacts with histones H4 and H2A. The interaction is dependent of the ability of RBBP7 to bind to the N-terminus of histones. Component of the histone H3.1 and H3.3 complexes.
Belongs to the HAT1 family.
研究領域
· Human Diseases > Substance dependence > Alcoholism.
参考文献
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