SENP1 Antibody - #AF0275

Fig. 7: Deficiency of miR-30a-5p promotes the interaction of TP53INP1 with p53 and TP53INP1 SUMOylation. a Representative immunoprecipitation detection of the interaction between p53 and TP53INP1 in the heart tissues of WT and KO mice. b Representative immunofluorescence images of p53 (green) and TP53INP1 (red) in NRCMs transfected with the control or miR-30a-5p inhibitor (scale bars = 50 μm). DAPI (blue) was used to counterstain the nucleus. c Heatmap of SUMOylation-related genes from RNA-seq data of NRCMs transfected with control or miR-30a-5p mimics. d qRT‒PCR analysis of SUMOylation-related genes in NRCMs transfected with control or miR-30a-5p mimics (n = 6 in each group). e qRT‒PCR analysis of SUMOylation-related genes in heart samples from WT and KO mice (n = 5 in each group). Representative western blotting images (f) and quantitative analysis (g) of SENP1 in NRCMs transfected with control or miR-30a-5p inhibitor (n = 3 in each group). h Representative immunoprecipitation blot showing the interaction of TP53INP1 with SUMO1 and p53 in the hearts of WT and KO mice. The data are presented as the means ± standard errors. Statistical significance was assessed using one-way ANOVA. WT wild-type, KO knockout, NC negative control, NRCMs neonatal rat cardiomyocytes, qRT‒PCR quantitative real‒time polymerase chain reaction.

Figure 1 Dynamic levels of SENP1 during OM-MSC differentiation (A and B) qPCR and western blot were utilized to measure SENP1 level in OM-MSCs, astrocytes, and neurons. Data are represented as means ± SD. Statistical difference was determined using one-way analysis of variance test followed by Tukey multiple-comparison post hoc analysis. n = 3. ∗p < 0.05, ∗∗p < 0.01.

Figure 1. Expression of SENP1 in liver sinusoidal endothelial cells following H-R. (A and B) Western blotting analysis. (C) Reverse transcription-quantitative polymerase chain reaction analysis. Data were presented as the mean ± SD (n=3); ***P

Fig. 2. After hypoxic culture of mouse LSECs, the expressions of SENP1,HIF-1a,and VEGF are all up-regulated.(A)(B)(C)real-time PCR showing SENP1 or HIF-1a or VEGF mRNA after a certain period of hypoxia culture.The date are presented as mean ± SEM.**p < 0.01,***p < 0.001,****p < 0.0001,n ¼ 3per group.(D)Western results showing the expression level of SENP1 and HIF-1a after a certain time of hypoxia culture.(E)(F) ratio of SENP1 or HIF-1a vs b-actin in western results.The date are presented as mean ± SEM.*p < 0.05,**p < 0.01,***p < 0.001,n ¼ 3 per group.(G) ELISA assay of VEGF production in LSECs after a certain time of hypoxia culture.The date are presented as mean ± SEM.****p < 0.0001,n ¼ 3 per group.