IRS1 Antibody - #AF6273

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製品: IRS1 Antibody
カタログ: AF6273
タンパク質の説明: Rabbit polyclonal antibody to IRS1
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat, Monkey
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 180kDa; 132kD(Calculated).
ユニプロット: P35568
RRID: AB_2835128

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説明書
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WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat,Monkey
予測:
Pig(91%), Zebrafish(88%), Bovine(91%), Horse(100%), Sheep(91%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
IRS1 Antibody detects endogenous levels of total IRS1.
RRID:
AB_2835128
引用形式: Affinity Biosciences Cat# AF6273, RRID:AB_2835128.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

HIRS 1; HIRS1; Insulin receptor substrate 1; IRS 1; IRS-1; IRS1; IRS1_HUMAN; OTTHUMP00000164234;

免疫原

免疫原:
Uniprot:

P35568(IRS1_HUMAN) >>Visit HPA database.

遺伝子(ID):
IRS1(3667)
タンパク質の説明:
IRS-1 is an adaptor protein that is one of the major substrates of the insulin receptor kinase. Contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain-containing proteins including phosphatidylinositol 3-kinase p85 subunit and GRB-2.
タンパク質配列:
MASPPESDGFSDVRKVGYLRKPKSMHKRFFVLRAASEAGGPARLEYYENEKKWRHKSSAPKRSIPLESCFNINKRADSKNKHLVALYTRDEHFAIAADSEAEQDSWYQALLQLHNRAKGHHDGAAALGAGGGGGSCSGSSGLGEAGEDLSYGDVPPGPAFKEVWQVILKPKGLGQTKNLIGIYRLCLTSKTISFVKLNSEAAAVVLQLMNIRRCGHSENFFFIEVGRSAVTGPGEFWMQVDDSVVAQNMHETILEAMRAMSDEFRPRSKSQSSSNCSNPISVPLRRHHLNNPPPSQVGLTRRSRTESITATSPASMVGGKPGSFRVRASSDGEGTMSRPASVDGSPVSPSTNRTHAHRHRGSARLHPPLNHSRSIPMPASRCSPSATSPVSLSSSSTSGHGSTSDCLFPRRSSASVSGSPSDGGFISSDEYGSSPCDFRSSFRSVTPDSLGHTPPARGEEELSNYICMGGKGPSTLTAPNGHYILSRGGNGHRCTPGTGLGTSPALAGDEAASAADLDNRFRKRTHSAGTSPTITHQKTPSQSSVASIEEYTEMMPAYPPGGGSGGRLPGHRHSAFVPTRSYPEEGLEMHPLERRGGHHRPDSSTLHTDDGYMPMSPGVAPVPSGRKGSGDYMPMSPKSVSAPQQIINPIRRHPQRVDPNGYMMMSPSGGCSPDIGGGPSSSSSSSNAVPSGTSYGKLWTNGVGGHHSHVLPHPKPPVESSGGKLLPCTGDYMNMSPVGDSNTSSPSDCYYGPEDPQHKPVLSYYSLPRSFKHTQRPGEPEEGARHQHLRLSTSSGRLLYAATADDSSSSTSSDSLGGGYCGARLEPSLPHPHHQVLQPHLPRKVDTAAQTNSRLARPTRLSLGDPKASTLPRAREQQQQQQPLLHPPEPKSPGEYVNIEFGSDQSGYLSGPVAFHSSPSVRCPSQLQPAPREEETGTEEYMKMDLGPGRRAAWQESTGVEMGRLGPAPPGAASICRPTRAVPSSRGDYMTMQMSCPRQSYVDTSPAAPVSYADMRTGIAAEEVSLPRATMAAASSSSAASASPTGPQGAAELAAHSSLLGGPQGPGGMSAFTRVNLSPNRNQSAKVIRADPQGCRRRHSSETFSSTPSATRVGNTVPFGAGAAVGGGGGSSSSSEDVKRHSSASFENVWLRPGELGGAPKEPAKLCGAAGGLENGLNYIDLDLVKDFKQCPQECTPEPQPPPPPPPHQPLGSGESSSTRRSSEDLSAYASISFQKQPEDRQ

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Rabbit
100
Pig
91
Bovine
91
Sheep
91
Zebrafish
88
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P35568 基板として

Site PTM Type Enzyme
Phosphorylation
S3 Phosphorylation
S24 Phosphorylation P17252 (PRKCA) , P53350 (PLK1) , P67870 (CSNK2B)
Y46 Phosphorylation
Y47 Phosphorylation
K52 Acetylation
K61 Acetylation
Y87 Phosphorylation
T88 Phosphorylation P67870 (CSNK2B)
S99 Phosphorylation P67870 (CSNK2B)
K169 Ubiquitination
Y183 Phosphorylation
S268 Phosphorylation O14920 (IKBKB)
S270 Phosphorylation Q13535 (ATR) , P23443 (RPS6KB1) , O14920 (IKBKB)
S272 Phosphorylation O14920 (IKBKB)
S273 Phosphorylation
S274 Phosphorylation O14920 (IKBKB)
S303 Phosphorylation
T305 Phosphorylation
S307 Phosphorylation Q02750 (MAP2K1) , Q05655 (PRKCD) , P45984 (MAPK9) , P23443 (RPS6KB1) , O14920 (IKBKB) , P45983 (MAPK8) , P42345 (MTOR)
T309 Phosphorylation
T311 Phosphorylation
S312 Phosphorylation P28482 (MAPK1) , O14733 (MAP2K7) , O14920 (IKBKB) , P45983 (MAPK8)
S315 Phosphorylation P45983 (MAPK8) , P45984 (MAPK9)
S323 Phosphorylation Q05655 (PRKCD) , P05771 (PRKCB) , Q05513 (PRKCZ)
S329 Phosphorylation
S330 Phosphorylation P67870 (CSNK2B)
T335 Phosphorylation
S337 Phosphorylation
S341 Phosphorylation O14920 (IKBKB)
S345 Phosphorylation O14920 (IKBKB)
S348 Phosphorylation
S350 Phosphorylation
T351 Phosphorylation
S362 Phosphorylation Q05655 (PRKCD)
S372 Phosphorylation
R373 Methylation
S374 Phosphorylation
S383 Phosphorylation
S385 Phosphorylation
S388 Phosphorylation
S391 Phosphorylation
S417 Phosphorylation
S419 Phosphorylation
Y431 Phosphorylation
S441 Phosphorylation Q05655 (PRKCD) , P05771 (PRKCB) , Q05513 (PRKCZ)
S444 Phosphorylation
T446 Phosphorylation
S449 Phosphorylation
T453 Phosphorylation
S463 Phosphorylation
Y465 Phosphorylation P08069 (IGF1R)
Y483 Phosphorylation
S486 Phosphorylation
T495 Phosphorylation
T498 Phosphorylation
T502 Phosphorylation P68400 (CSNK2A1)
S503 Phosphorylation
T525 Phosphorylation
S527 Phosphorylation P23443 (RPS6KB1) , O14920 (IKBKB)
T530 Phosphorylation
S531 Phosphorylation O14920 (IKBKB)
T533 Phosphorylation
S574 Phosphorylation Q05655 (PRKCD)
S581 Phosphorylation
S603 Phosphorylation
S604 Phosphorylation
T605 Phosphorylation
T608 Phosphorylation
Y612 Phosphorylation P23458 (JAK1) , P08069 (IGF1R) , P45984 (MAPK9)
S616 Phosphorylation P27361 (MAPK3) , P28482 (MAPK1) , P45983 (MAPK8) , Q05655 (PRKCD)
S624 Phosphorylation
S629 Phosphorylation P31749 (AKT1)
Y632 Phosphorylation P45984 (MAPK9) , P23458 (JAK1) , P08069 (IGF1R)
S636 Phosphorylation P23443 (RPS6KB1) , O75116 (ROCK2) , P45983 (MAPK8) , P28482 (MAPK1)
S639 Phosphorylation P28482 (MAPK1)
Y662 Phosphorylation P08069 (IGF1R) , P23458 (JAK1)
S666 Phosphorylation
S668 Phosphorylation
S672 Phosphorylation
S680 Phosphorylation
S694 Phosphorylation
Y695 Phosphorylation
Y732 Phosphorylation P23458 (JAK1) , P08069 (IGF1R)
S736 Phosphorylation
S766 Phosphorylation
K772 Methylation
K772 Ubiquitination
S794 Phosphorylation Q9H0K1 (SIK2)
T811 Phosphorylation P67870 (CSNK2B)
T847 Phosphorylation
T859 Phosphorylation
S862 Phosphorylation
K867 Ubiquitination
S892 Phosphorylation
Y896 Phosphorylation P06241 (FYN) , P08069 (IGF1R) , P06213 (INSR)
T936 Phosphorylation
T938 Phosphorylation
Y941 Phosphorylation P08069 (IGF1R)
S974 Phosphorylation O75116 (ROCK2)
S984 O-Glycosylation
S984 Phosphorylation
S985 O-Glycosylation
Y989 Phosphorylation P06213 (INSR) , P08069 (IGF1R)
S1005 Phosphorylation
S1011 O-Glycosylation
Y1012 Phosphorylation
S1025 Phosphorylation
S1037 Phosphorylation
S1041 Phosphorylation
S1043 Phosphorylation
T1045 Phosphorylation
S1078 Phosphorylation
S1084 Phosphorylation
S1100 Phosphorylation P17612 (PRKACA)
S1101 Phosphorylation P05129 (PRKCG) , Q05513 (PRKCZ) , Q04759 (PRKCQ) , Q05655 (PRKCD) , P23443 (RPS6KB1)
T1103 Phosphorylation
S1105 Phosphorylation
T1107 Phosphorylation
S1109 Phosphorylation
T1111 Phosphorylation
T1116 Phosphorylation
S1142 Phosphorylation
S1143 Phosphorylation
S1145 Phosphorylation
Y1179 Phosphorylation P06213 (INSR) , P08069 (IGF1R) , P06241 (FYN)
S1222 Phosphorylation P17612 (PRKACA)
S1223 Phosphorylation P17612 (PRKACA)
Y1229 Phosphorylation P06213 (INSR) , P08069 (IGF1R)

研究背景

機能:

May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity).

PTMs:

Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor (By similarity). Phosphorylation of Tyr-896 is required for GRB2-binding (By similarity). Phosphorylated by ALK. Phosphorylated at Ser-270, Ser-307, Ser-636 and Ser-1101 by RPS6KB1; phosphorylation induces accelerated degradation of IRS1. Phosphorylated on tyrosine residues in response to insulin. In skeletal muscles, dephosphorylated on Tyr-612 by TNS2 under anabolic conditions; dephosphorylation results in the proteasomal degradation of IRS1.

Ubiquitinated by the Cul7-RING(FBXW8) complex in a mTOR-dependent manner, leading to its degradation: the Cul7-RING(FBXW8) complex recognizes and binds IRS1 previously phosphorylated by S6 kinase (RPS6KB1 or RPS6KB2).

サブユニット構造:

Interacts with UBTF and PIK3CA (By similarity). Interacts (via phosphorylated YXXM motifs) with PIK3R1 (By similarity). Interacts with ROCK1 and FER (By similarity). Interacts (via PH domain) with PHIP (By similarity). Interacts with GRB2 (By similarity). Interacts with SOCS7. Interacts (via IRS-type PTB domain) with IGF1R and INSR (via the tyrosine-phosphorylated NPXY motif). Interacts with ALK. Interacts with EIF2AK2/PKR (By similarity). Interacts with GKAP1 (By similarity). Interacts with DGKZ in the absence of insulin; insulin stimulation decreases this interaction (By similarity). Found in a ternary complex with DGKZ and PIP5K1A in the absence of insulin stimulation (By similarity).

研究領域

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Type II diabetes mellitus.

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway - multiple species.   (View pathway)

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

· Organismal Systems > Excretory system > Aldosterone-regulated sodium reabsorption.

参考文献

1). Arsenic induces hepatic insulin resistance via mtROS-NLRP3 inflammasome pathway. JOURNAL OF HAZARDOUS MATERIALS, 2020 (PubMed: 32544768) [IF=13.6]

Application: WB    Species: rat    Sample: liver

Fig.2 The effect of NaAsO2 on mitophagy, ox-mtDNA and NLRP3 inflammation in rats liver. Male SD rats were treated with 2.5, 5 mg/kg of NaAsO2 for 3 months. Liver coefficient (A). The level of serum ALT and AST were determined by commercial reagent kits (B-C). H&E staining of liver sections after NaAsO2 administration (D). scale bar = 500 μm. NAS in rat liver (E). Cytosolic fractions were analyzed by Western blot analysis. GAPDH was used as an internal control. The relative expression of MPO was shown as the percentage of GAPDH (F-G). The level of ox￾mtDNA was measured with an ELISA kit (H). Mitochondria fractions were analyzed by Western blot analysis. VDAC1, mitochondria marker protein, was used as an Journal Pre-proof internal control. The protein level and densitometric analyses of PINK1, Parkin, LC3B in rats liver tissues (I-L). The protein level and densitometric analyses of NLRP3, IL-1β, IL-18 expressed in rat liver tissues (M-T). Results are mean ± SEM of 5 rats. *P < 0.05 compare with the control group.
2). Insulin promotes the bone formation capability of human dental pulp stem cells through attenuating the IIS/PI3K/AKT/mTOR pathway axis. Stem cell research & therapy, 2024 (PubMed: 39075596) [IF=7.5]

Application: WB    Species: Human    Sample: DPSCs

Fig. 4 10− 6 M insulin inhibits the gene and protein expressions of the IIS-related receptors and substrates in human DPSCs. A 10− 6 M insulin down-regulated the mRNA levels of INSR, IGF1R, and IRS1 in DPSCs at day 3 and day 7. B 10− 6 M insulin inhibited the protein expressions of INSR, IGF1R, and IRS1 in DPSCs at day 7. Representative western blotting (left) and quantification analysis (right). Full-length blots/gels are presented in Supplementary Fig. 2. Data are expressed as the mean ± SD of n = 3.
3). Anti-diabetes effect of chronic intermittent hypobaric hypoxia through improving liver insulin resistance in diabetic rats. LIFE SCIENCES, 2016 (PubMed: 26883978) [IF=6.1]

Application: WB    Species:    Sample:

4). Probiotic Yogurt Alleviates High-Fat Diet-Induced Lipid Accumulation and Insulin Resistance in Mice via the Adiponectin Pathway. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2023 (PubMed: 36695046) [IF=6.1]
5). Silencing of ANGPTL8 Alleviates Insulin Resistance in Trophoblast Cells. Frontiers in Endocrinology, 2021 (PubMed: 34163433) [IF=5.2]

Application: WB    Species: mouse    Sample: placenta

FIGURE 1 | Angiopoietin like-8 (ANGPTL8) was increased in serum and placenta tissues of gestational diabetes mellitus (GDM) mice.(J) Western blot was used to determine the levels of insulin signaling related molecules, p-IRb(Tyr1361), IRb, p-IRS-1(Ser307), p-IRS-1(Tyr896), IRS-1, p-Akt and Akt in placenta tissues.

Application: WB    Species: Mice    Sample: serum and placenta tissue

Figure 1 Angiopoietin like-8 (ANGPTL8) was increased in serum and placenta tissues of gestational diabetes mellitus (GDM) mice. (A) The mice were treated as described in the chart. (B) The body weight of mice in normal fat diet (NFD) and high fat diet (HFD) groups. (C) Oral glucose tolerance test (OGTT) was performed at gestational day (GD)0.5, 11.5 and 16.5. (D, E) Fasting blood glucose and insulin levels were measured at GD18.5. (F) Homeostasis model assessment insulin resistance (HOMA-IR) was calculated as follow: HOMA-IR= blood glucose (mM)×blood insulin (mU/l)/22.5. (G) The contents of triglyceride (TG), total cholesterol (TC), high density lipoprotein (HDL-C) and low density lipoprotein (LDL-C) in serum were detected. (H) HE staining was performed to detect the pathological changes in labyrinth zone of placenta tissues. (I) Periodic acid Schiff (PAS) staining was carried out to detect the glycogen accumulation in labyrinth zone of placenta tissues. (J) Western blot was used to determine the levels of insulin signaling related molecules, p-IRβ(Tyr1361), IRβ, p-IRS-1(Ser307), p-IRS-1(Tyr896), IRS-1, p-Akt and Akt in placenta tissues. (K) The expression levels of glucose transporter 1 (GLUT1) and GLUT4 in placenta tissues. (L) The serum level of ANGPTL8 in mice. (M, N) The mRNA and protein levels of ANGPTL8 in placenta tissues. (the scale bar represents 100 μm; **p < 0.01, ***p < 0.001 vs. NFD).
6). Periplocymarin Induced Colorectal Cancer Cells Apoptosis Via Impairing PI3K/AKT Pathway. Frontiers in Oncology, 2021 (PubMed: 34900704) [IF=4.7]

Application: WB    Species: Human    Sample: HCT 116 RKO cells

Figure 6 Periplocymarin (PPM) modulates gene expression involved in PI3K/AKT signaling pathway. PPM treatment altered the expression levels of IRS1, PI3K, AKT, p-PI3K, and p-AKT in HCT 116 (A) and RKO cells (B). Values are shown as means ± SEM. *P < 0.05, **P < 0.01, vs. untreated control group.

Application: IHC    Species: Mice    Sample: tumors

Figure 7 The effect of periplocymarin (PPM) on colorectal cancer cells in nude mice. (A) Images of tumors derived from mice in each group. (B) Tumor volumes were calculated in each group every 3 days from days 1 to 21. (C) Tumor weights were measured on day 21. (D) Hematoxylin/eosin-stained sections of tumor tissues derived from mice in each treatment group were presented. Magnification: 200×. Scale bar, 150 μm. The expression of Bax, cleaved caspase-3, IRS1, p-PI3K, and p-AKT in tumor tissues of mice from each treatment group was examined by immunohistochemistry. Magnification: 400×. Scale bar, 70 μm. *P < 0.05, **P < 0.01, vs. untreated control group. # P < 0.05, vs. 5-FU treatment group. & P < 0.05, vs. PPM treatment group. n =6 per group.
7). Metabolomics Study of Whole-body Vibration on Lipid Metabolism of Skeletal Muscle in Aging Mice. INTERNATIONAL JOURNAL OF SPORTS MEDICINE, 2020 (PubMed: 33124015) [IF=2.5]
8). The Aqueous Extract of Gynura divaricata (L.) DC. Improves Glucose and Lipid Metabolism and Ameliorates Type 2 Diabetes Mellitus. Evidence-based Complementary and Alternative Medicine, 2018 (PubMed: 29599810)
9). Exploring the anti-diabetic effects and the underlying mechanisms of ethyl acetate extract from Sophora flavescens by integrating network pharmacology and pharmacological evaluation. Traditional Medicine Research, 2022

Application: WB    Species: Rat    Sample:

Figure 7 Western blot analysis of the proteins. (A) Representative bands of IRS-1, p-PI3K, PI3K, p-AKT, AKT and GLUT4 were shown.

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