ALG3 Antibody - #DF14333

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製品: ALG3 Antibody
カタログ: DF14333
タンパク質の説明: Rabbit polyclonal antibody to ALG3
アプリケーション: WB IHC IF/ICC
Cited expt.: WB
反応性: Human, Mouse
分子量: 55kD; 50kD(Calculated).
ユニプロット: Q92685

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
クローナリティ:
Polyclonal
特異性:
ALG3 Antibody detects endogenous levels of total ALG3.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Asparagine linked glycosylation protein 3 homolog; Asparagine-linked glycosylation 3, alpha-1,3- mannosyltransferase homolog (S. cerevisiae); Carbohydrate deficient glycoprotein syndrome type IV; CDG1D; CDGS4; D16Ertd36e; Dol-P-Man dependent alpha(1-3)-mannosyltransferase; Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase; Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase; Dolichyl-phosphate-mannose--glycolipid alpha-mannosyltransferase; NOT; Not56; Not56 like protein; NOT56L;

免疫原

免疫原:

A synthesized peptide derived from Human ALG3.

Uniprot:

Q92685(ALG3_HUMAN) >>Visit HPA database.

遺伝子(ID):
ALG3(10195)
タンパク質配列:
MAAGLRKRGRSGSAAQAEGLCKQWLQRAWQERRLLLREPRYTLLVAACLCLAEVGITFWVIHRVAYTEIDWKAYMAEVEGVINGTYDYTQLQGDTGPLVYPAGFVYIFMGLYYATSRGTDIRMAQNIFAVLYLATLLLVFLIYHQTCKVPPFVFFFMCCASYRVHSIFVLRLFNDPVAMVLLFLSINLLLAQRWGWGCCFFSLAVSVKMNVLLFAPGLLFLLLTQFGFRGALPKLGICAGLQVVLGLPFLLENPSGYLSRSFDLGRQFLFHWTVNWRFLPEALFLHRAFHLALLTAHLTLLLLFALCRWHRTGESILSLLRDPSKRKVPPQPLTPNQIVSTLFTSNFIGICFSRSLHYQFYVWYFHTLPYLLWAMPARWLTHLLRLLVLGLIELSWNTYPSTSCSSAALHICHAVILLQLWLGPQPFPKSTQHSKKAH

研究背景

機能:

Adds the first Dol-P-Man derived mannose in an alpha-1,3 linkage to Man5GlcNAc2-PP-Dol.

細胞の位置付け:

Endoplasmic reticulum membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the glycosyltransferase 58 family.

研究領域

· Metabolism > Glycan biosynthesis and metabolism > N-Glycan biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

参考文献

1). ɑ1,3-mannosyltransferase promotes the malignant progression of bladder cancer through activating TNF signaling pathway. European journal of medical research, 2025 (PubMed: 40312703) [IF=2.8]

Application: WB    Species: human    Sample: T24 and UMUC3 cells

Fig. 7 ALG3 promoted cell proliferation, migration, and invasion of bladder cancer cells. A, B Quantitative real-time PCR analysis of ALG3 expression in T24 and UMUC3 cells after transfection with ALG3 cDNA (A) or ALG3 siRNA (B). C, D Western blot analysis of ALG3 expression in T24 and UMUC3 cells after transfection with ALG3 cDNA (C) or ALG3 siRNA (D) for 48 h. E, F CCK8 assays assessing changes in cell proliferation ability after treatment with ALG3 cDNA (E) or ALG3 siRNA (F). G, H Colony formation assays evaluating cell proliferation ability after transfection with ALG3 cDNA (G) or ALG3 siRNA (H). I, J Transwell assays measuring cell migration and invasion abilities after treatment with ALG3 cDNA (I) or ALG3 siRNA (J). **P < 0.01, ***P < 0.001

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