ADD1 Antibody - #AF6276
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF6276, RRID:AB_2835129.
折りたたみ／展開
ADD A; ADD1; ADDA; ADDA_HUMAN; Adducin 1 (alpha); Alpha adducin; Alpha-adducin; Erythrocyte adducin alpha subunit; Erythrocyte adducin subunit alpha; MGC3339; MGC44427; OTTHUMP00000151163; OTTHUMP00000151164; OTTHUMP00000151165; OTTHUMP00000218664; OTTHUMP00000218665; ADD1; ADD2; ADD3; ADDA; ADDB; ADDL; Adducin 1 (alpha); Adducin 1; Adducin 2 (beta); Adducin 2; Adducin 3 (gamma); Adducin 3; Adducin like; Alpha adducin; Beta adducin;
免疫原
Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.
P35612 ADDB_HUMAN:Expressed mainly in brain, spleen, kidney cortex and medulla, and heart. Also expressed in human umbilical vein endothelial cells, human vascular smooth muscle cells, kidney tubular cells and K-562 cell line.
- P35611 ADDA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNGDSRAAVVTSPPPTTAPHKERYFDRVDENNPEYLRERNMAPDLRQDFNMMEQKKRVSMILQSPAFCEELESMIQEQFKKGKNPTGLLALQQIADFMTTNVPNVYPAAPQGGMAALNMSLGMVTPVNDLRGSDSIAYDKGEKLLRCKLAAFYRLADLFGWSQLIYNHITTRVNSEQEHFLIVPFGLLYSEVTASSLVKINLQGDIVDRGSTNLGVNQAGFTLHSAIYAARPDVKCVVHIHTPAGAAVSAMKCGLLPISPEALSLGEVAYHDYHGILVDEEEKVLIQKNLGPKSKVLILRNHGLVSVGESVEEAFYYIHNLVVACEIQVRTLASAGGPDNLVLLNPEKYKAKSRSPGSPVGEGTGSPPKWQIGEQEFEALMRMLDNLGYRTGYPYRYPALREKSKKYSDVEVPASVTGYSFASDGDSGTCSPLRHSFQKQQREKTRWLNSGRGDEASEEGQNGSSPKSKTKWTKEDGHRTSTSAVPNLFVPLNTNPKEVQEMRNKIREQNLQDIKTAGPQSQVLCGVVMDRSLVQGELVTASKAIIEKEYQPHVIVSTTGPNPFTTLTDRELEEYRREVERKQKGSEENLDEAREQKEKSPPDQPAVPHPPPSTPIKLEEDLVPEPTTGDDSDAATFKPTLPDLSPDEPSEALGFPMLEKEEEAHRPPSPTEAPTEASPEPAPDPAPVAEEAAPSAVEEGAAADPGSDGSPGKSPSKKKKKFRTPSFLKKSKKKSDS
- P35612 ADDB_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSEETVPEAASPPPPQGQPYFDRFSEDDPEYMRLRNRAADLRQDFNLMEQKKRVTMILQSPSFREELEGLIQEQMKKGNNSSNIWALRQIADFMASTSHAVFPTSSMNVSMMTPINDLHTADSLNLAKGERLMRCKISSVYRLLDLYGWAQLSDTYVTLRVSKEQDHFLISPKGVSCSEVTASSLIKVNILGEVVEKGSSCFPVDTTGFCLHSAIYAARPDVRCIIHLHTPATAAVSAMKWGLLPVSHNALLVGDMAYYDFNGEMEQEADRINLQKCLGPTCKILVLRNHGVVALGDTVEEAFYKIFHLQAACEIQVSALSSAGGVENLILLEQEKHRPHEVGSVQWAGSTFGPMQKSRLGEHEFEALMRMLDNLGYRTGYTYRHPFVQEKTKHKSEVEIPATVTAFVFEEDGAPVPALRQHAQKQQKEKTRWLNTPNTYLRVNVADEVQRSMGSPRPKTTWMKADEVEKSSSGMPIRIENPNQFVPLYTDPQEVLEMRNKIREQNRQDVKSAGPQSQLLASVIAEKSRSPSTESQLMSKGDEDTKDDSEETVPNPFSQLTDQELEEYKKEVERKKLELDGEKETAPEEPGSPAKSAPASPVQSPAKEAETKSPLVSPSKSLEEGTKKTETSKAATTEPETTQPEGVVVNGREEEQTAEEILSKGLSQMTTSADTDVDTSKDKTESVTSGPMSPEGSPSKSPSKKKKKFRTPSFLKKSKKKEKVES
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P35611/P35612 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S5 | Phosphorylation | Uniprot | |
| T11 | Phosphorylation | Uniprot | |
| S12 | Phosphorylation | P06493 (CDK1) | Uniprot |
| T16 | Phosphorylation | Uniprot | |
| K21 | Ubiquitination | Uniprot | |
| Y24 | Phosphorylation | Uniprot | |
| R27 | Methylation | Uniprot | |
| Y35 | Phosphorylation | Uniprot | |
| K55 | Ubiquitination | Uniprot | |
| S59 | Phosphorylation | Uniprot | |
| S64 | Phosphorylation | Uniprot | |
| K140 | Acetylation | Uniprot | |
| K140 | Ubiquitination | Uniprot | |
| K143 | Ubiquitination | Uniprot | |
| K148 | Ubiquitination | Uniprot | |
| K288 | Ubiquitination | Uniprot | |
| T331 | Phosphorylation | Uniprot | |
| S334 | Phosphorylation | Uniprot | |
| K348 | Ubiquitination | Uniprot | |
| S353 | Phosphorylation | Uniprot | |
| S355 | Phosphorylation | P06493 (CDK1) | Uniprot |
| S358 | Phosphorylation | Uniprot | |
| T364 | Phosphorylation | Uniprot | |
| S366 | Phosphorylation | Uniprot | |
| Y393 | Phosphorylation | Uniprot | |
| Y395 | Phosphorylation | Uniprot | |
| K406 | Ubiquitination | Uniprot | |
| S408 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S423 | Phosphorylation | Uniprot | |
| S427 | Phosphorylation | Uniprot | |
| T429 | Phosphorylation | Uniprot | |
| S431 | Phosphorylation | Uniprot | |
| S436 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| K439 | Ubiquitination | Uniprot | |
| T445 | Phosphorylation | Q9Y5S2 (CDC42BPB) , Q13464 (ROCK1) , O75116 (ROCK2) | Uniprot |
| S457 | Phosphorylation | Uniprot | |
| S464 | Phosphorylation | Uniprot | |
| S465 | Phosphorylation | Uniprot | |
| T480 | Phosphorylation | Q13464 (ROCK1) , O75116 (ROCK2) | Uniprot |
| S481 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| T482 | Phosphorylation | Uniprot | |
| S483 | Phosphorylation | Uniprot | |
| K515 | Ubiquitination | Uniprot | |
| C525 | S-Nitrosylation | Uniprot | |
| R531 | Methylation | Uniprot | |
| S532 | Phosphorylation | Uniprot | |
| Y550 | Phosphorylation | Uniprot | |
| T559 | Phosphorylation | Uniprot | |
| Y575 | Phosphorylation | Uniprot | |
| S586 | Phosphorylation | Uniprot | |
| S600 | Phosphorylation | Uniprot | |
| S613 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T614 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S645 | Phosphorylation | Uniprot | |
| S669 | Phosphorylation | Uniprot | |
| T671 | Phosphorylation | Uniprot | |
| S678 | Phosphorylation | Uniprot | |
| S707 | Phosphorylation | Uniprot | |
| S710 | Phosphorylation | Uniprot | |
| S714 | Phosphorylation | Uniprot | |
| S716 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| T724 | Phosphorylation | Uniprot | |
| S726 | Phosphorylation | Q05513 (PRKCZ) , O14757 (CHEK1) , P17252 (PRKCA) , Q05655 (PRKCD) , P17612 (PRKACA) | Uniprot |
| S731 | Phosphorylation | Uniprot | |
| S735 | Phosphorylation | Uniprot | |
| S737 | Phosphorylation | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Phosphorylation | Uniprot | |
| T5 | Phosphorylation | Uniprot | |
| S11 | Phosphorylation | Uniprot | |
| Y31 | Phosphorylation | Uniprot | |
| T55 | Phosphorylation | Uniprot | |
| S60 | Phosphorylation | Uniprot | |
| S62 | Phosphorylation | Uniprot | |
| Y377 | Phosphorylation | Uniprot | |
| S396 | Phosphorylation | Uniprot | |
| Y440 | Phosphorylation | Uniprot | |
| S455 | Phosphorylation | Uniprot | |
| K464 | Acetylation | Uniprot | |
| S472 | Phosphorylation | Uniprot | |
| Y489 | Phosphorylation | P06241 (FYN) | Uniprot |
| S512 | Phosphorylation | Uniprot | |
| S522 | Phosphorylation | Uniprot | |
| S528 | Phosphorylation | Uniprot | |
| S530 | Phosphorylation | Uniprot | |
| S532 | Phosphorylation | Uniprot | |
| T533 | Phosphorylation | Uniprot | |
| S535 | Phosphorylation | Uniprot | |
| K576 | Acetylation | Uniprot | |
| K583 | Acetylation | Uniprot | |
| T585 | Phosphorylation | Uniprot | |
| S592 | Phosphorylation | Uniprot | |
| K595 | Acetylation | Uniprot | |
| S596 | Phosphorylation | Uniprot | |
| S600 | Phosphorylation | Uniprot | |
| S604 | Phosphorylation | Q00535 (CDK5) | Uniprot |
| T611 | Phosphorylation | Uniprot | |
| S613 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| S617 | Phosphorylation | Q00535 (CDK5) | Uniprot |
| S619 | Phosphorylation | Uniprot | |
| S621 | Phosphorylation | Uniprot | |
| T626 | Phosphorylation | Uniprot | |
| T631 | Phosphorylation | Uniprot | |
| S632 | Phosphorylation | Uniprot | |
| T675 | Phosphorylation | Uniprot | |
| K681 | Acetylation | Uniprot | |
| K683 | Acetylation | Uniprot | |
| T684 | Phosphorylation | Uniprot | |
| S693 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| S697 | Phosphorylation | Uniprot | |
| S699 | Phosphorylation | Uniprot | |
| S701 | Phosphorylation | Q00535 (CDK5) | Uniprot |
| S703 | Phosphorylation | Uniprot | |
| T711 | Phosphorylation | Uniprot | |
| S713 | Phosphorylation | O14757 (CHEK1) , P17612 (PRKACA) , Q05513 (PRKCZ) , P17252 (PRKCA) | Uniprot |
| S718 | Phosphorylation | Uniprot | |
| S726 | Phosphorylation | P17252 (PRKCA) | Uniprot |
研究背景
Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
Cytoplasm>Cytoskeleton. Cell membrane>Peripheral membrane protein>Cytoplasmic side.
Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.
Heterodimer of an alpha and a beta subunit or an alpha and a gamma subunit.
Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.
Belongs to the aldolase class II family. Adducin subfamily.
Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to the erythrocyte membrane receptor SLC2A1/GLUT1 and may therefore provide a link between the spectrin cytoskeleton to the plasma membrane. Binds to calmodulin. Calmodulin binds preferentially to the beta subunit.
The N-terminus is blocked.
Cytoplasm>Cytoskeleton. Cell membrane>Peripheral membrane protein>Cytoplasmic side.
Expressed mainly in brain, spleen, kidney cortex and medulla, and heart. Also expressed in human umbilical vein endothelial cells, human vascular smooth muscle cells, kidney tubular cells and K-562 cell line.
Heterodimer of an alpha and a beta subunit. Found in a complex with ADD2, DMTN and SLC2A1. Interacts with SLC2A1.
Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.
Belongs to the aldolase class II family. Adducin subfamily.
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