STAT3 Antibody | Affinity Biosciences

製品:	STAT3 Antibody
カタログ:	AF6294
タンパク質の説明:	Rabbit polyclonal antibody to STAT3
アプリケーション:	WB IHC IF/ICC
Cited expt.:	WB, IHC, IF/ICC
反応性:	Human, Mouse, Rat
予測:	Pig, Bovine, Horse, Sheep, Rabbit, Chicken
分子量:	86kDa; 88kD(Calculated).
ユニプロット:	P40763
RRID:	AB_2835144

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サイズ	価格	在庫状況
100ul	$280	在庫あり
200ul	$350	在庫あり

プロトコル

製品説明

ソース:

Rabbit

アプリケーション:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:

Human,Mouse,Rat

予測:

Pig(91%), Bovine(91%), Horse(91%), Sheep(82%), Rabbit(91%), Chicken(91%)

クローナリティ:

Polyclonal

特異性:

STAT3 Antibody detects endogenous levels of total STAT3.

RRID:

AB_2835144
引用形式: Affinity Biosciences Cat# AF6294, RRID:AB_2835144.

コンジュゲート:

Unconjugated.

精製:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

別名:

折りたたみ／展開

1110034C02Rik; Acute Phase Response Factor; Acute-phase response factor; ADMIO; APRF; AW109958; DNA binding protein APRF; FLJ20882; HIES; MGC16063; Signal transducer and activator of transcription 3 (acute phase response factor); Signal transducer and activator of transcription 3; STAT 3; Stat3; STAT3_HUMAN;

免疫原

免疫原:

A synthesized peptide derived from human STAT3, corresponding to a region within C-terminal amino acids.

Uniprot:

P40763(STAT3_HUMAN) >>Visit HPA database.

遺伝子(ID):

STAT3(6774)

発現特異性:

P40763 STAT3_HUMAN:

Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

タンパク質の説明:

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators.

タンパク質配列:

P40763 STAT3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAQWNQLQQLDTRYLEQLHQLYSDSFPMELRQFLAPWIESQDWAYAASKESHATLVFHNLLGEIDQQYSRFLQESNVLYQHNLRRIKQFLQSRYLEKPMEIARIVARCLWEESRLLQTAATAAQQGGQANHPTAAVVTEKQQMLEQHLQDVRKRVQDLEQKMKVVENLQDDFDFNYKTLKSQGDMQDLNGNNQSVTRQKMQQLEQMLTALDQMRRSIVSELAGLLSAMEYVQKTLTDEELADWKRRQQIACIGGPPNICLDRLENWITSLAESQLQTRQQIKKLEELQQKVSYKGDPIVQHRPMLEERIVELFRNLMKSAFVVERQPCMPMHPDRPLVIKTGVQFTTKVRLLVKFPELNYQLKIKVCIDKDSGDVAALRGSRKFNILGTNTKVMNMEESNNGSLSAEFKHLTLREQRCGNGGRANCDASLIVTEELHLITFETEVYHQGLKIDLETHSLPVVVISNICQMPNAWASILWYNMLTNNPKNVNFFTKPPIGTWDQVAEVLSWQFSSTTKRGLSIEQLTTLAEKLLGPGVNYSGCQITWAKFCKENMAGKGFSFWVWLDNIIDLVKKYILALWNEGYIMGFISKERERAILSTKPPGTFLLRFSESSKEGGVTFTWVEKDISGKTQIQSVEPYTKQQLNNMSFAEIIMGYKIMDATNILVSPLVYLYPDIPKEEAFGKYCRPESQEHPEADPGSAAPYLKTKFICVTPTTCSNTIDLPMSPRTLDSLMQFGNNGEGAEPSAGGQFESLTFDMELTSECATSPM

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Bovine

Chicken

Rabbit

Sheep

Zebrafish

Dog

Xenopus

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

P40763_STAT3_HUMAN

機能:

Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors. Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. Acts as a regulator of inflammatory response by regulating differentiation of naive CD4(+) T-cells into T-helper Th17 or regulatory T-cells (Treg): deacetylation and oxidation of lysine residues by LOXL3, leads to disrupt STAT3 dimerization and inhibit its transcription activity. Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1. Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation (By similarity). May play an apoptotic role by transctivating BIRC5 expression under LEP activation. Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity. Plays a crucial role in basal beta cell functions, such as regulation of insulin secretion (By similarity).

PTMs:

Tyrosine phosphorylated upon stimulation with EGF. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity). Activated through tyrosine phosphorylation by BMX. Tyrosine phosphorylated in response to IL6, IL11, LIF, CNTF, KITLG/SCF, CSF1, EGF, PDGF, IFN-alpha, LEP and OSM. Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity. ARL2BP may participate in keeping the phosphorylated state of STAT3 within the nucleus. Upon LPS challenge, phosphorylated within the nucleus by IRAK1. Upon erythropoietin treatment, phosphorylated on Ser-727 by RPS6KA5. Phosphorylation at Tyr-705 by PTK6 or FER leads to an increase of its transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates IL6/interleukin-6 signaling.

Acetylated on lysine residues by CREBBP. Deacetylation by LOXL3 leads to disrupt STAT3 dimerization and inhibit STAT3 transcription activity. Oxidation of lysine residues to allysine on STAT3 preferentially takes place on lysine residues that are acetylated.

Some lysine residues are oxidized to allysine by LOXL3, leading to disrupt STAT3 dimerization and inhibit STAT3 transcription activity. Oxidation of lysine residues to allysine on STAT3 preferentially takes place on lysine residues that are acetylated.

(Microbial infection) Phosphorylated on Tyr-705 in the presence of S.typhimurium SarA.

S-palmitoylated by ZDHHC19 in SH2 putative lipid-binding pockets, leading to homodimerization. Nuclear STAT3 is highly palmitoylated (about 75%) compared with cytoplasmic STAT3 (about 20%).

S-stearoylated, probably by ZDHHC19.

細胞の位置付け:

Cytoplasm. Nucleus.
Note: Shuttles between the nucleus and the cytoplasm. Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3. Identified in a complex with LYN and PAG1.

組織特異性:

Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

タンパク質ファミリー:

Belongs to the transcription factor STAT family.

研究領域

· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells. (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Pancreatic cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia. (View pathway)

· Human Diseases > Cancers: Specific types > Non-small cell lung cancer. (View pathway)

· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).

· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation. (View pathway)

· Organismal Systems > Endocrine system > Prolactin signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

参考文献

1). Gut microbial metabolite 4-hydroxybenzeneacetic acid drives colorectal cancer progression via accumulation of immunosuppressive PMN-MDSCs. The Journal of clinical investigation, 2025 (PubMed: 40179015) [IF=13.3]

Application: IF/ICC Species: human Sample: SW480 cells

Figure 6 4-HPA promotes the transcription of CXCL3 regulated by STAT3 in CRC cells. (A) Chemical structures of 4-HPA, FITC, and FITC-labeled -HPA. (B) Detection of FITC-labeled 4-HPA by fluorescence confocal microscopy in RKO and SW480 cells. Scale bar: 50 μm. (C) Predicted transcription factors for CXCL3. (D) WB analysis of the effects of 4-HPA on transcription factors in CRC cell lines. (E) The binding sites of STAT3 and CXCL3 were confirmed using a dual-luciferase reporter assay (n = 3). (F) Transcriptional regulation of CXCL3 by p-STAT3 was detected using ChIP assays. (G) WB analysis of the JAK2/STAT3 signaling pathway and CXCL3 in SW480 cells. (H–J) WB analysis of CXCL3 in SW480 cells. (K and L) IF assays visualizing the subcellular localization of STAT3 and p-STAT3 in SW480 cells treated with 4-HPA. Scale bar: 50 μm. Data represent the mean ± SD of 3 independent experiments. Statistical analyses were conducted using Student’s t test. ***P < 0.0005. MUT, mutation; NC, negative control.

2). ITGA5-expressing tumor cells interact with Schwann cells to drive nerve growth factor-mediated immunosuppression of NK cells. Molecular therapy : the journal of the American Society of Gene Therapy, 2025 (PubMed: 40734271) [IF=12.1]

Application: WB Species: Mouse Sample: Schwann cells

Figure 4 ITGA5-induced NGF secretion in Schwann cells (A) Representative figures of GFAP (red) and p75NTR (green) staining in tumor tissues. Scale bar: 20 μm. (B) Quantification of GFAP+ cells and GFAP+p75NTR+ cells. (C) Flow cytometry analysis of GFAP and c-Jun expression in Schwann cells (SCs). (D) Relative expression of Ngf mRNA. The y axis represents the relative expression levels using the housekeeping gene Gapdh as the standard. CCs, cancer cells. n = 3 technical replicates. (E) Quantification of secreted NGF in CCs and SCs by ELISA (n = 3 technical replicates). (F) Predicted interaction of fibronectin to protein itga5. Nodes represent individual proteins, with ITGA5/Itga5 as the central node. Edges indicate predicted interactions, with line thickness reflecting confidence scores. Line colors denote evidence types: curated databases (sky blue), experimental interactions (purple), co-expression (black), etc. Functional partners are labeled with gene symbols. (G) Molecular model of the interaction between ITGA5 (blue) and FN1 (red). At right is an enlarged view of interacting amino acid residues. (H) Expression levels of Fn1 mRNA in NC and OE groups. (I) Relative expression of Ngf mRNA. The y axis represents the relative expression levels using the housekeeping gene Gapdh as the standard. NC, negative control. (J) Quantification of secreted NGF in si-Ctrl and si-Fn1 SCs detected by ELISA (n = 3 technical replicates). (K) Transcript levels of reparative SC markers in samples from each group. (L) KEGG pathway enrichment analysis of genes significantly downregulated by si-Fn1 treatment. (M) Representative western blot images showing the expression of STAT3, p-STAT3, JAK2, p-JAK2, and GAPDH as the loading control. Quantitative analysis of band intensity normalized to GAPDH. CM, conditioned medium. Each bar represents the mean (SEM) from three independent experiments. ∗p < 0.05; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001. Statistical comparisons were performed using two-tailed independent samples t tests.

3). Targeting CDCP1 boost CD8+ T cells-mediated cytotoxicity in cervical cancer via the JAK/STAT signaling pathway. Journal for immunotherapy of cancer, 2024 (PubMed: 39455095) [IF=10.9]

4). Lacc1-engineered extracellular vesicles reprogram mitochondrial metabolism to alleviate inflammation and cartilage degeneration in TMJ osteoarthritis. Journal of nanobiotechnology, 2025 (PubMed: 40186254) [IF=10.2]

5). Aligned electrospun poly(l-lactide) nanofibers facilitate wound healing by inhibiting macrophage M1 polarization via the JAK-STAT and NF-κB pathways. JOURNAL OF NANOBIOTECHNOLOGY, 2022 (PubMed: 35883095) [IF=10.2]

Application: WB Species: Mice Sample:

Fig. 3 The underlying mechanism by which aligned fibers affected macrophage polarization. A Venn diagram showing differentially expressed genes. B KEGG pathway analysis between the A20 and R20 groups. C Heatmap of differentially expressed genes among the three groups. D Heatmap of macrophage polarization-related genes between the A20 and R20 groups. E Volcano diagram of differentially expressed genes. F Western blot analysis of the NF-κB signaling pathway. G Immunofluorescence staining showing the nuclear translocation of NF-κB p65. The nucleus is stained blue, and NF-κB p65 protein is stained red. H Western blot images and semiquantitative analysis of the JAK-STAT signaling pathway (*p < 0.05, **p < 0.01, n = 3)

6). Cancer-associated fibroblasts secrete CSF3 to promote TNBC progression via enhancing PGM2L1-dependent glycolysis reprogramming. Cell Death & Disease, 2025 [IF=9.6]

7). CD11b-modified ROS/pH-responsive nanoparticles co-deliver Dioscin and siRNA to improve cardiac repair after myocardial infarction by reducing neutrophil recruitment. Materials today. Bio, 2025 (PubMed: 40977834) [IF=8.7]

Application: WB Species: Mouse Sample:

Fig. 3. Intracellular uptake of Dio/siICAM-1@MSN@PDA-CD11b in vitro. (A–B) Uptake of Dio/siICAM-1@MSN@PDA-CD11b by neutrophils and cardiomyocytes at different time points was analyzed by CLSM (A) and flow cytometry (B). Scale bar: 10 μm. (C) The mRNA expression levels of ICAM-1 in neutrophils under different treatment groups. (D) The protein expression of NF-κB/STAT3/p38-MAPK pathway in neutrophils under different treatment groups. ∗P < 0.05, ∗∗P < 0.01. N = 3 for each experiment.

8). Tofacitinib Promotes Functional Recovery after Spinal Cord Injury by Regulating Microglial Polarization via JAK/STAT Signaling Pathway. International Journal of Biological Sciences, 2023 (PubMed: 37781508) [IF=8.2]

9). CYSLTR1 antagonist inhibits Th17 cell differentiation by regulating the NF-κB signaling for the treatment of psoriasis. International journal of biological sciences, 2024 (PubMed: 38617532) [IF=8.2]

10). Alhagi camelorum seed polysaccharide alleviates methylglyoxal-induced skin damage via antioxidant and anti-inflammatory actions. International journal of biological macromolecules, 2025 (PubMed: 40311972) [IF=7.7]

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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STAT3 Antibody - #AF6294

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