STAT5A Antibody - #AF6303
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF6303, RRID:AB_2835152.
折りたたみ／展開
Mammary gland factor; MGF; Signal transducer and activator of transcription 5A; STA5A_HUMAN; STAT 5; STAT 5A; STAT5; STAT5A;
免疫原
- P42229 STA5A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGWIQAQQLQGDALRQMQVLYGQHFPIEVRHYLAQWIESQPWDAIDLDNPQDRAQATQLLEGLVQELQKKAEHQVGEDGFLLKIKLGHYATQLQKTYDRCPLELVRCIRHILYNEQRLVREANNCSSPAGILVDAMSQKHLQINQTFEELRLVTQDTENELKKLQQTQEYFIIQYQESLRIQAQFAQLAQLSPQERLSRETALQQKQVSLEAWLQREAQTLQQYRVELAEKHQKTLQLLRKQQTIILDDELIQWKRRQQLAGNGGPPEGSLDVLQSWCEKLAEIIWQNRQQIRRAEHLCQQLPIPGPVEEMLAEVNATITDIISALVTSTFIIEKQPPQVLKTQTKFAATVRLLVGGKLNVHMNPPQVKATIISEQQAKSLLKNENTRNECSGEILNNCCVMEYHQATGTLSAHFRNMSLKRIKRADRRGAESVTEEKFTVLFESQFSVGSNELVFQVKTLSLPVVVIVHGSQDHNATATVLWDNAFAEPGRVPFAVPDKVLWPQLCEALNMKFKAEVQSNRGLTKENLVFLAQKLFNNSSSHLEDYSGLSVSWSQFNRENLPGWNYTFWQWFDGVMEVLKKHHKPHWNDGAILGFVNKQQAHDLLINKPDGTFLLRFSDSEIGGITIAWKFDSPERNLWNLKPFTTRDFSIRSLADRLGDLSYLIYVFPDRPKDEVFSKYYTPVLAKAVDGYVKPQIKQVVPEFVNASADAGGSSATYMDQAPSPAVCPQAPYNMYPQNPDHVLDQDGEFDLDETMDVARHVEELLRRPMDSLDSRLSPPAGLFTSARGSLS
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P42229 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y22 | Phosphorylation | Uniprot | |
| K70 | Ubiquitination | Uniprot | |
| K71 | Ubiquitination | Uniprot | |
| K84 | Acetylation | Uniprot | |
| K84 | Ubiquitination | Uniprot | |
| K86 | Ubiquitination | Uniprot | |
| Y90 | Phosphorylation | Uniprot | |
| T92 | O-Glycosylation | Uniprot | |
| T92 | Phosphorylation | Uniprot | |
| Y114 | Phosphorylation | Uniprot | |
| S127 | Phosphorylation | Uniprot | |
| S128 | Phosphorylation | Uniprot | |
| K163 | Ubiquitination | Uniprot | |
| K164 | Ubiquitination | Uniprot | |
| S193 | Phosphorylation | Uniprot | |
| K207 | Ubiquitination | Uniprot | |
| K232 | Ubiquitination | Uniprot | |
| K235 | Ubiquitination | Uniprot | |
| K242 | Ubiquitination | Uniprot | |
| T245 | Phosphorylation | Uniprot | |
| K256 | Sumoylation | Uniprot | |
| K256 | Ubiquitination | Uniprot | |
| K336 | Sumoylation | Uniprot | |
| K343 | Ubiquitination | Uniprot | |
| K359 | Ubiquitination | Uniprot | |
| K370 | Ubiquitination | Uniprot | |
| T372 | Phosphorylation | Uniprot | |
| S375 | Phosphorylation | Uniprot | |
| K380 | Ubiquitination | Uniprot | |
| K384 | Acetylation | Uniprot | |
| K384 | Sumoylation | Uniprot | |
| K384 | Ubiquitination | Uniprot | |
| S473 | Phosphorylation | Uniprot | |
| K516 | Sumoylation | Uniprot | |
| K516 | Ubiquitination | Uniprot | |
| K527 | Ubiquitination | Uniprot | |
| K586 | Ubiquitination | Uniprot | |
| K644 | Ubiquitination | Uniprot | |
| T647 | Phosphorylation | Uniprot | |
| T648 | Phosphorylation | Uniprot | |
| Y668 | Phosphorylation | Uniprot | |
| K675 | Ubiquitination | Uniprot | |
| K681 | Ubiquitination | Uniprot | |
| Y682 | Phosphorylation | Uniprot | |
| Y683 | Phosphorylation | Uniprot | |
| K689 | Ubiquitination | Uniprot | |
| Y694 | Phosphorylation | Q06187 (BTK) , P12931 (SRC) , P52333 (JAK3) , P06239 (LCK) , P06213 (INSR) , P07948 (LYN) , P43405 (SYK) , Q13882 (PTK6) , O60674 (JAK2) , P08631 (HCK) | Uniprot |
| K696 | Acetylation | Uniprot | |
| K696 | Sumoylation | Uniprot | |
| K696 | Ubiquitination | Uniprot | |
| K700 | Acetylation | Uniprot | |
| Y720 | Phosphorylation | Uniprot | |
| S726 | Phosphorylation | Uniprot | |
| T757 | Phosphorylation | Uniprot | |
| S774 | Phosphorylation | Uniprot | |
| S777 | Phosphorylation | Uniprot | |
| S780 | Phosphorylation | Q13153 (PAK1) , P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
| S792 | Phosphorylation | Uniprot | |
| S794 | Phosphorylation | Uniprot |
研究背景
Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.
Tyrosine phosphorylated in response to KITLG/SCF, IL2, IL3, IL7, IL15, CSF2/GMCSF, GH1, PRL, EPO and THPO (By similarity). Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity). Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity (By similarity). Tyrosine phosphorylated in response to signaling via activated FLT3; wild-type FLT3 results in much weaker phosphorylation than constitutively activated mutant FLT3. Alternatively, can be phosphorylated by JAK2 at Tyr-694.
ISGylated.
Cytoplasm. Nucleus.
Note: Translocated into the nucleus in response to phosphorylation.
Forms a homodimer or a heterodimer with a related family member. Binds NR3C1 (By similarity). Interacts with NCOA1 and SOCS7. Interacts with ERBB4.
Belongs to the transcription factor STAT family.
研究領域
· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)
· Environmental Information Processing > Signal transduction > ErbB signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Viral > Hepatitis B.
· Human Diseases > Infectious diseases: Viral > Measles.
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia. (View pathway)
· Human Diseases > Cancers: Specific types > Acute myeloid leukemia. (View pathway)
· Human Diseases > Cancers: Specific types > Non-small cell lung cancer. (View pathway)
· Organismal Systems > Immune system > Th1 and Th2 cell differentiation. (View pathway)
· Organismal Systems > Immune system > Th17 cell differentiation. (View pathway)
· Organismal Systems > Endocrine system > Prolactin signaling pathway. (View pathway)
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