p27 Kip1 Antibody - #AF6324

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製品: p27 Kip1 Antibody
カタログ: AF6324
タンパク質の説明: Rabbit polyclonal antibody to p27 Kip1
アプリケーション: WB IF/ICC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Bovine, Sheep, Rabbit, Dog, Chicken
分子量: 27kDa; 22kD(Calculated).
ユニプロット: P46527
RRID: AB_2835182

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(90%)
クローナリティ:
Polyclonal
特異性:
p27 Kip1 Antibody detects endogenous levels of total p27 Kip1.
RRID:
AB_2835182
引用形式: Affinity Biosciences Cat# AF6324, RRID:AB_2835182.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

AA408329; AI843786; Cdki1b; CDKN 1B; CDKN 4; CDKN1B; CDKN4; CDN1B_HUMAN; Cyclin Dependent Kinase Inhibitor 1B; Cyclin dependent kinase inhibitor p27; Cyclin-dependent kinase inhibitor 1B (p27, Kip1); Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; Cyclin-dependent kinase inhibitor p27 Kip1; KIP 1; KIP1; MEN1B; MEN4; OTTHUMP00000195098; OTTHUMP00000195099; p27; p27 Kip1; P27-like cyclin-dependent kinase inhibitor; p27Kip1;

免疫原

免疫原:

A synthesized peptide derived from human p27 Kip1, corresponding to a region within C-terminal amino acids.

Uniprot:

P46527(CDN1B_HUMAN) >>Visit HPA database.

遺伝子(ID):
CDKN1B(1027)
発現特異性:
P46527 CDN1B_HUMAN:

Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.

タンパク質の説明:
This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1.
タンパク質配列:
MSNVRVSNGSPSLERMDARQAEHPKPSACRNLFGPVDHEELTRDLEKHCRDMEEASQRKWNFDFQNHKPLEGKYEWQEVEKGSLPEFYYRPPRPPKGACKVPAQESQDVSGSRPAAPLIGAPANSEDTHLVDPKTDPSDSQTGLAEQCAGIRKRPATDDSSTQNKRANRTEENVSDGSPNAGSVEQTPKKPGLRRRQT

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
90
Pig
0
Horse
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.

PTMs:

Phosphorylated; phosphorylation occurs on serine, threonine and tyrosine residues. Phosphorylation on Ser-10 is the major site of phosphorylation in resting cells, takes place at the G(0)-G(1) phase and leads to protein stability. Phosphorylation on other sites is greatly enhanced by mitogens, growth factors, cMYC and in certain cancer cell lines. The phosphorylated form found in the cytoplasm is inactivate. Phosphorylation on Thr-198 is required for interaction with 14-3-3 proteins. Phosphorylation on Thr-187, by CDK1 and CDK2 leads to protein ubiquitination and proteasomal degradation. Tyrosine phosphorylation promotes this process. Phosphorylation by PKB/AKT1 can be suppressed by LY294002, an inhibitor of the catalytic subunit of PI3K. Phosphorylation on Tyr-88 and Tyr-89 has no effect on binding CDK2, but is required for binding CDK4. Dephosphorylated on tyrosine residues by G-CSF.

Ubiquitinated; in the cytoplasm by the KPC complex (composed of RNF123/KPC1 and UBAC1/KPC2) and, in the nucleus, by SCF(SKP2). The latter requires prior phosphorylation on Thr-187. Ubiquitinated; by a TRIM21-containing SCF(SKP2)-like complex; leads to its degradation.

Subject to degradation in the lysosome. Interaction with SNX6 promotes lysosomal degradation (By similarity).

細胞の位置付け:

Nucleus. Cytoplasm. Endosome.
Note: Nuclear and cytoplasmic in quiescent cells. AKT- or RSK-mediated phosphorylation on Thr-198, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression. Mitogen-activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression. Phosphorylation on Ser-10 facilitates nuclear export. Translocates to the nucleus on phosphorylation of Tyr-88 and Tyr-89. Colocalizes at the endosome with SNX6; this leads to lysosomal degradation (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.

タンパク質ファミリー:

A peptide sequence containing only AA 28-79 retains substantial Kip1 cyclin A/CDK2 inhibitory activity.

Belongs to the CDI family.

研究領域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

参考文献

1). Skp2 modulates proliferation, senescence and tumorigenesis of glioma. Cancer Cell International, 2020 (PubMed: 32165861) [IF=5.8]

Application: WB    Species: mouse    Sample: U87, U138, and LNZ308 cells

Fig. 2| Downregulation of Skp2 attenuated in vitro and in vivo cell proliferation. a On Western blot analyses, the protein level of Skp2 in glioma cell lines (A172, U87, U118, U373, LNZ308, U138 and U343), as compared to normal astrocytes (AST), was enhanced. b Skp2 was successfully knocked-down by two shRNA fragments in U87, U138, and LNZ308 cells compared with the negative control shLuc. The protein levels of the downstream molecules ­p21Cip1/Waf1 and ­p27Kip1 were increased upon the knockdown of Skp2 in all three cell lines.
2). Compound Kushen injection attenuates angiotensin II‑mediated heart failure by inhibiting the PI3K/Akt pathway. International Journal of Molecular Medicine, 2023 (PubMed: 36734284) [IF=5.7]

Application: WB    Species: Rat    Sample: H9C2 cells

Figure 7 Expression of apoptosis-related proteins in vitro. (A) The results of western blot analysis for each protein. (B-I) The expression of representative proteins. (B) p-Akt expression, (C) t-Akt expression, (D) p-Akt/t-Akt ratio, (E) Bcl-2 expression, (F) cyclin D1 expression, (G) PI3K expression, (H) Bax expression, and (I) p27 expression in the different groups. Data are presented as the mean ± SEM. One-way ANOVA and post-hoc Tukey's multiple comparisons test were used to determine statistically significant differences (n=3 per group). *P
3). Protective effect of Huanglian Pingwei San on DSS-induced ulcerative colitis in mice through amelioration of the inflammatory response and oxidative stress. Frontiers in pharmacology, 2024 (PubMed: 39697546) [IF=5.6]

Application: WB    Species: Mouse    Sample: colon tissues

FIGURE 5. HLPWS inhibited apoptosis-related proteins. (A) Protein expression of P53, P21, cleaved caspase 3, p-H2AX, and P27 in colon tissues was determined by Western blotting. (B) Quantitative analysis of the Western blot results (n = 3). (C) Expression of cleaved caspase three and p-H2AX in the colon tissues of UC mice detected by IHC (original magnifications, ×200; scale bar = 100 μm). The results are expressed as the means ± SEM. ## p < 0.01, # p < 0.05 vs. the control group; ** p < 0.01, * p < 0.05 vs. the DSS group.
4). Over-Expression and Prognostic Significance of FATP5, as a New Biomarker, in Colorectal Carcinoma. Frontiers in Molecular Biosciences, 2022 (PubMed: 35155561) [IF=5.0]

Application: WB    Species: Human    Sample: CRC cells

FIGURE 6 siFATP5 facilitates cell growth and cell cycle G2/M phase transition. FATP5 expression was detected after transfection in CRC cells by qRT-PCR (A) and western blotting (B,C) CCK-8 assays were used to detect cell viability of CRC cells after transfection. (D,E) Flow cytometric analysis was used to detect cell cycle distribution of CRC cells after transfection. (F) The expression of cycle-related protein. *p < 0.05, **p < 0.01.
5). Ligustrazine Suppresses Platelet-Derived Growth Factor-BB-Induced Pulmonary Artery Smooth Muscle Cell Proliferation and Inflammation by Regulating the PI3K/AKT Signaling Pathway. The American journal of Chinese medicine, 2021 (PubMed: 33622214) [IF=4.8]
6). Regulation of the PTEN/PI3K/AKT pathway in RCC using the active compounds of natural products in vitro. Molecular Medicine Reports, 2021 (PubMed: 34490473) [IF=3.4]

Application: WB    Species: Human    Sample: RCC cells

Figure 3. Naringenin arrests cell cycle progression of renal cell carcinoma cells in the G2 phase. 786-O and OS-RC-2 cells were treated with naringenin (0, 4 or 8 µM) for 48 h. (A) Cell cycle distribution was determined using flow cytometry. (B) Western blot analysis of cyclin E1, cyclin A2, cyclin B1, cyclin D1, P27 and P21 expression levels. Data are presented as the mean ± SD. *P<0.05, **P<0.01 vs. Abs. Abs, absolute ethanol.

Application: WB    Species: human    Sample: 786‑O and OS‑RC‑2 cells

Figure 3.| Naringenin arrests cell cycle progression of renal cell carcinoma cells in the G2 phase. 786‑O and OS‑RC‑2 cells were treated with naringenin (0, 4 or 8 µM) for 48 h. (A) Cell cycle distribution was determined using flow cytometry. (B) Western blot analysis of cyclin E1, cyclin A2, cyclin B1, cyclin D1, P27 and P21 expression levels. Data are presented as the mean ± SD. * P<0.05, **P<0.01 vs. Abs. Abs, absolute ethanol.
7). IDH1 gene mutation activates Smad signaling molecules to regulate the expression levels of cell cycle and biological rhythm genes in human glioma U87‑MG cells. Molecular Medicine Reports, 2021 (PubMed: 33760141) [IF=3.4]
8). Dihydroartemisinin inhibits the tumorigenesis and invasion of gastric cancer by regulating STAT1/KDR/MMP9 and P53/BCL2L1/CASP3/7 pathways. PATHOLOGY RESEARCH AND PRACTICE, 2021 (PubMed: 33370709) [IF=2.9]

Application: WB    Species: mice    Sample: GC cells

Fig. 6. DHA regulated p53/BCL2L1/Caspase-3/-7 and STAT1/KDR/MMP9 signaling pathways. (A) qRT-PCR analysis of the effects of DHA on the expression levels of BCL2, BAX, CKD4, p53/BCL2L1/Caspase-3/-7 and STAT1/KDR/MMP9 in GC cells. (B) WB analysis of the effects of DHA on the expression of apoptosis and cycle-related proteins including cleaved-caspase 3, caspase 3, caspase 7, BCL2L1, p27 and p53. (C) WB analysis of the effects of DHA on the expression of p-STAT1, STAT1, p-KDR, KDR and MMP9.
9). LncRNA GACAT3 acts as a competing endogenous RNA of HMGA1 and alleviates cucurbitacin B-induced apoptosis of gastric cancer cells. GENE, 2018 (PubMed: 30098426) [IF=2.6]

Application: WB    Species: human    Sample: SGC-7901 cells

Fig. 3. |GACAT3 and HMGA1 promote cell growth by inhibiting p21 and p27.qRT-PCR and western blot analyses of p27 and p21 levels in gastric cancer cells after GACAT3 or HMGA1 siRNA-mediated knockdown. The mRNA expression levels of p27 and p21 in SGC-7901 (A) and BGC-823 (B) cells. Western blotting results and relative protein levels of p27, p21, and HMGA1 in SGC-7901 (C) and BGC-823 (D)cells. Data represent the mean ± S.D. of three biological replicates. *p < 0.05; **p < 0.01.
10). Fusobacterium nucleatum Caused DNA Damage and Promoted Cell Proliferation by the Ku70/p53 Pathway in Oral Cancer Cells. DNA AND CELL BIOLOGY, 2020 (PubMed: 31765243) [IF=2.6]

Application: WB    Species: human    Sample: Tca8113 tongue squamous cell

FIG. 3.| The protein expression of p27, wild p53, and Ku70 in response to F. nucleatum infection (A).
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