NMDAR1 Antibody - #AF6406

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製品: NMDAR1 Antibody
カタログ: AF6406
タンパク質の説明: Rabbit polyclonal antibody to NMDAR1
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Dog, Chicken, Xenopus
分子量: 130kDa; 105kD(Calculated).
ユニプロット: Q05586
RRID: AB_2835236

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説明書
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プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Dog(100%), Chicken(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
NMDAR1 Antibody detects endogenous levels of total NMDAR1.
RRID:
AB_2835236
引用形式: Affinity Biosciences Cat# AF6406, RRID:AB_2835236.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

GluN1; Glutamate [NMDA] receptor subunit zeta-1; Glutamate receptor ionotropic N methyl D aspartate 1; Glutamate receptor ionotropic, N-methyl-D aspartate, subunit 1; glutamate receptor ionotropic, NMDA 1; Grin1; MRD8; N methyl D aspartate receptor; N methyl D aspartate receptor channel subunit zeta 1; N methyl D aspartate receptor subunit NR1; N-methyl-D-aspartate receptor subunit NR1; NMD-R1; NMDA 1; NMDA R1; NMDA receptor 1; NMDA1; NMDAR; NMDZ1_HUMAN; NR1;

免疫原

免疫原:
Uniprot:

Q05586(NMDZ1_HUMAN) >>Visit HPA database.

遺伝子(ID):
GRIN1(2902)
タンパク質の説明:
The protein encoded by this gene is a critical subunit of N-methyl-D-aspartate receptors, members of the glutamate receptor channel superfamily which are heteromeric protein complexes with multiple subunits arranged to form a ligand-gated ion channel. These subunits play a key role in the plasticity of synapses, which is believed to underlie memory and learning. The gene consists of 21 exons and is alternatively spliced, producing transcript variants differing in the C-terminus.
タンパク質配列:
MSTMRLLTLALLFSCSVARAACDPKIVNIGAVLSTRKHEQMFREAVNQANKRHGSWKIQLNATSVTHKPNAIQMALSVCEDLISSQVYAILVSHPPTPNDHFTPTPVSYTAGFYRIPVLGLTTRMSIYSDKSIHLSFLRTVPPYSHQSSVWFEMMRVYSWNHIILLVSDDHEGRAAQKRLETLLEERESKAEKVLQFDPGTKNVTALLMEAKELEARVIILSASEDDAATVYRAAAMLNMTGSGYVWLVGEREISGNALRYAPDGILGLQLINGKNESAHISDAVGVVAQAVHELLEKENITDPPRGCVGNTNIWKTGPLFKRVLMSSKYADGVTGRVEFNEDGDRKFANYSIMNLQNRKLVQVGIYNGTHVIPNDRKIIWPGGETEKPRGYQMSTRLKIVTIHQEPFVYVKPTLSDGTCKEEFTVNGDPVKKVICTGPNDTSPGSPRHTVPQCCYGFCIDLLIKLARTMNFTYEVHLVADGKFGTQERVNNSNKKEWNGMMGELLSGQADMIVAPLTINNERAQYIEFSKPFKYQGLTILVKKEIPRSTLDSFMQPFQSTLWLLVGLSVHVVAVMLYLLDRFSPFGRFKVNSEEEEEDALTLSSAMWFSWGVLLNSGIGEGAPRSFSARILGMVWAGFAMIIVASYTANLAAFLVLDRPEERITGINDPRLRNPSDKFIYATVKQSSVDIYFRRQVELSTMYRHMEKHNYESAAEAIQAVRDNKLHAFIWDSAVLEFEASQKCDLVTTGELFFRSGFGIGMRKDSPWKQNVSLSILKSHENGFMEDLDKTWVRYQECDSRSNAPATLTFENMAGVFMLVAGGIVAGIFLIFIEIAYKRHKDARRKQMQLAFAAVNVWRKNLQDRKSGRAEPDPKKKATFRAITSTLASSFKRRRSSKDTSTGGGRGALQNQKDTVLPRRAIEREEGQLQLCSRHRES

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Xenopus
100
Chicken
100
Sheep
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q05586 基板として

Site PTM Type Enzyme
K51 Acetylation
S446 Phosphorylation
T683 Phosphorylation
C744 S-Nitrosylation
C798 S-Nitrosylation
Y837 Phosphorylation P12931 (SRC)
S889 Phosphorylation
S890 Phosphorylation P05129 (PRKCG) , Q9HC98 (NEK6)
S896 Phosphorylation
S897 Phosphorylation
T900 Phosphorylation
S901 Phosphorylation
T902 Phosphorylation

研究背景

機能:

Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+). Sensitivity to glutamate and channel kinetics depend on the subunit composition.

PTMs:

NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein. Cell junction>Synapse>Postsynaptic cell membrane. Cell junction>Synapse>Postsynaptic density.
Note: Enriched in postsynaptic plasma membrane and postsynaptic densities.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Heterotetramer. Forms heterotetrameric channels composed of two zeta subunits (GRIN1), and two epsilon subunits (GRIN2A, GRIN2B, GRIN2C or GRIN2D) (in vitro). Can also form heterotetrameric channels that contain at least one zeta subunit (GRIN1), an epsilon subunit, plus GRIN3A or GRIN3B (in vitro). In vivo, the subunit composition may vary in function of the expression levels of the different subunits. Found in a complex with GRIN2A or GRIN2B, GRIN3A and PPP2CB (By similarity). Found in a complex with GRIN2A or GRIN2B and GRIN3B (By similarity). Interacts with SNX27 (via PDZ domain); the interaction is required for recycling to the plasma membrane when endocytosed and prevent degradation in lysosomes (By similarity). Interacts with DLG4 and MPDZ. Interacts with LRFN1 and LRFN2 (By similarity). Interacts with MYZAP. Found in a complex with DLG4 and PRR7 (By similarity). Found in a complex with GRIN2B and PRR7. Interacts with PRR7; the interaction is reduced following NMDA receptor activity.

タンパク質ファミリー:

A hydrophobic region that gives rise to the prediction of a transmembrane span does not cross the membrane, but is part of a discontinuously helical region that dips into the membrane and is probably part of the pore and of the selectivity filter.

Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR1/GRIN1 subfamily.

研究領域

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Substance dependence > Cocaine addiction.

· Human Diseases > Substance dependence > Amphetamine addiction.

· Human Diseases > Substance dependence > Alcoholism.

· Organismal Systems > Environmental adaptation > Circadian entrainment.

· Organismal Systems > Nervous system > Long-term potentiation.

· Organismal Systems > Nervous system > Glutamatergic synapse.

参考文献

1). STK24 modulates excitatory synaptic transmission in epileptic hippocampal neurons. CNS Neuroscience & Therapeutics, 2020 (PubMed: 32436359) [IF=5.5]

Application: WB    Species: mouse    Sample: hippocampus

FIGURE 5| STK24 is a potential binding partner of the NMDAR complex in the hippocampus of PTZ-kindled mice. A, STK24 interacts with the NMDAR complex, but not with the AMPAR complex. Hippocampal lysates from PTZkindled mice were immunoprecipitated with STK24 antibody and incubated with corresponding antibodies (IB: immunoblotting, IP: immunoprecipitation). B–F, Lysates from hippocampus of PTZkindled mice were immunoprecipitated with antibodies against NR1(B), NR2A(C), NR2B(D), GluR1(E), GluR2(F), and incubated with corresponding antibodies
2). Adverse effects of iron deficiency anemia on pregnancy outcome and offspring development and intervention of three iron supplements. Scientific Reports, 2021 (PubMed: 33446747) [IF=4.6]

Application: WB    Species: rat    Sample: liver tissues

Figure 5. Iron related indexes and neural development of ofspring rats afer iron supplement treatment. Western blot analysis for FTL and Tf in liver (a–c) and spleen (d–f). Morris water maze test for day 1 escape latency (g) and day 2 escape latency (h). Western blot analysis for FTL and Tf in brain (i–k) and hippocampus (l–n), and for SYN1, NMDAR, PSD-95 in hippocampus (o–r). Te quantifcation of western blotting was provided in supplementary material. FTL ferritin light chain, Tf transferrin, SYN1 synapsin 1, NMDAR N-methyl-D-aspartate receptor, PSD-95 postsynaptic density protein 95. Data of Western blot analysis (mean±SD) are expressed as the ratio of the relative contents between the value from IDA group and NG group and six iron treatment groups (n=3). Te relative contents of target proteins were quantifed using the ratio between the optical density (OD) of target protein and the amount of the housekeeping protein GAPDH. **p<0.01, compared with NG, #p<0.05, ##p<0.01, compared with IDAG. One-way ANOVA followed by Tukey multiple comparison test was used for comparison among 8 diferent groups.
3). Emotional and Cognitive Impairment Induced by Sleep Deprivation Due to Altered Synaptic Plasticity in the Rat Insula. , 2020

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